GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS ECOTN90 1196 bp ds-DNA BCT 27-MAY-1991
DEFINITION Transposon Tn903 (which codes for kanamycin resistance in E. coli).
ACCESSION V00621
KEYWORDS drug resistance gene; insertion sequence; kanamycin resistance;
unidentified reading frame.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1196)
AUTHORS Grindley,N.D. and Joyce,C.M.
TITLE Genetic and DNA sequence analysis of the kanamycin resistance
transposon Tn903
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 7176-7180 (1980)
STANDARD full automatic
COMMENT SWISS-PROT; P00551; KKA1$ECOLI.
From EMBL entry ISTN90; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 1..18
/note="inverted repeat of 1057 to 1040"
CDS complement(60..980)
/note="reading frame (unknown protein)"
/codon_start=980
CDS 507..847
/note="reading frame (unknown protein)"
/codon_start=507
misc_feature 930..939
/note="direct repeat of 2 to 10"
misc_feature 963..972
/note="inverted repeat of 1039 to 1029"
CDS 1162..>1194
/note="reading frame (kanamycin resistance) (1194 is 2nd
base in codon)"
/codon_start=1162
BASE COUNT 279 a 308 c 299 g 310 t
ORIGIN
1 ggctttgttg aataaatcag atttcgggta agtctccccc gtagcgggtt gtgttttcag
61 gcaatacgca cgctttcagg catacctgct ttcgtcattt tgttcagcgc tcgtaccagg
121 gccatagcct ccgcaacctg accatcgtag tcacgcagcg tcagtgaacc cccgaacagc
181 tgttttaccc ggtacatcgc cgtttccgct atcgagcgac ggttgtaatc tgttgtccat
241 ttccaccgcg cattactccc ggtcattcgc tgattagcca ctgcacggtt acggtctgca
301 tattcaccgg gccagtaacc cgcacctttt cggggaggga taagcgcgct gattttctta
361 cgccgcagtt catcgtgaca gagccgggtg tcgtaagcgc cgtctgccga tgctgccctg
421 atttttctgt gagtctgccg gataagaccc gggaaggctt ctgagtcggt cacattgttc
481 agcgacaggt cagcgcagat gatttcatgt gttttactgt caacggcgag atgcagctta
541 cgccagatac ggcggcgttc ctggccatgc tttttgactt tccattcgcc ttcaccaaag
601 accttcagcc cggtggaatc aatcaccaga tgcgcgattt caccccgggt gaacgttttg
661 aaactgatat taaccgactt tgcccgcctg ctgacacagc tgtaatccgg gcagcgcaac
721 ggaacattca tcagtgtaaa aatggaatca ataaagccct gcgcagcgcg cagggtcagc
781 ctgaatacgc gtttaatgac cagcacagtc gtgatggcaa ggtcagaata gcgctgaggt
841 ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc
901 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt
961 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc
1021 ttcaactcag caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga
1081 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
1141 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgag
//