GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS KPNPULB 4091 bp ds-DNA BCT 27-MAY-1991
DEFINITION Klebsiella pneumoniae pulA gene for pullulanase
ACCESSION X52181 M32702
KEYWORDS pulA gene; pullulanase.
SOURCE Klebsiella pneumoniae DNA.
ORGANISM Klebsiella pneumoniae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4091)
AUTHORS Kornacker,M.G. and Pugsley,A.P.
TITLE Molecular characterization of pulA and its product, pullulanase, a
secreted enzyme of Klebsiella pneumoniae UNF5023
JOURNAL Mol. Microbiol. 4, 73-85 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P07206; PULA$KLEPN.
Draft entry and computer-readable sequence for [1] kindly submitted
by A.P.Pugsley, 20-DEC-1989.
From EMBL entry KPPULA; dated 26-JUL-1990.
FEATURES Location/Qualifiers
CDS 512..3781
/note="pullulanase (pulA) precursor"
/codon_start=512
CDS 512..568
/note="pullulanase signal peptide"
/codon_start=512
CDS 569..3781
/note="pullulanase"
/codon_start=569
BASE COUNT 887 a 1156 c 1222 g 826 t
ORIGIN
1 aatgcgttat ccgtaaagtt taccgggaga attattttcc aggttaattg cgcgacgtgc
61 tggcagataa aaaataatat gatggaggtc acaatatgag gcgcgtaatt aattattttt
121 ttatttggta ttgttaatct catcacggaa ttatgcattt ttattccttt gctcattcta
181 tttccctcag caaaaggtac cgccctaccg atatgggtgg ggcatttttt atttttgagc
241 gtgcttattg cgggaatctg cgtaggtcgc tgcgaatcat aaagagtttg gctattgcgg
301 tcgtcattcc ctttttattt tttctcctcc ggccgcctac gcccccggag gaggagtgaa
361 tggatgattg aacctaagag agcaagaata tctaattatc gacgctttaa agatatgctc
421 gtcatttctt catggggctt aagtgcgcca ggaatagttt cgcgtatata acacttcata
481 taatttaatc agcatgtata gggactatcg aatgctcaga tatacccgta atgccctggt
541 tctgggatcg ctggttttac ttagcggctg cgataacgga tcctcttctt cttcctctgg
601 aaatcccgac actcctgata atcaggatgt ggttgtacgt ttacctgacg ttgcggtgcc
661 cggcgaagcg gtcacggcgg tagaaaacca ggcggtcatc cacctggtgg atattgccgg
721 catcaccagc agtagcgcgg cggactacag cagcaaaaac ctctatctct ggaacaacga
781 aacctgcgac gcgctgagcg cgccggtggc cgactggaat gacgtcagca ccacgccgag
841 cggcagcgat aaatatggcc cgtactgggt gattccgctc aacaaagaga gcggttgtat
901 caacgtcatt gtccgcgacg gcaccgataa gctgatcgat agcgacctgc gggtggcgtt
961 tggcgacttc accgatcgta cggtatcggt gattgccggt aacagcgcgg tctatgactc
1021 ccgcgccgac gccttccgcg ccgcttttgg cgtcgcgctg gccgaagccc actgggtcga
1081 caaaaatacg ctgctgtggc cgggcgggca ggataagccc atcgtgcgcc tctactacag
1141 ccacagcagt aaagtggcgg ccgacggcga aggcaaattt accgaccgct acctgaagct
1201 gacgccgact accgtcagcc agcaggtcag catgcgcttc ccgcaccttt ccagctatgc
1261 ggcctttaag ctgccggaca acgccaacgt ggacgagctg ctgcagggcg aaacggtcgc
1321 cattgccgcc gcagaggacg ggatcctgat ttccgccacc caggtgcaga cggcgggcgt
1381 cctggatgac gcctatgccg aagcggcgga agccctgagc tacggcgcgc agctggccga
1441 cggcggcgtc accttccgcg tttgggcgcc gactgcccag caggtggatg tggtggtcta
1501 cagcgccgat aagaaagtca ttggcagcca tccgatgacc cgcgacagcg cttccggcgc
1561 gtggtcatgg cagggcggca gcgatctgaa aggcgcgttc taccgctatg cgatgacggt
1621 ttatcacccg cagtcgcgta aggttgaaca gtacgaagtc accgacccgt atgcgcatag
1681 cctgtcgacc aactcggagt acagccaggt tgtcgatctc aacgacagtg ccctgaagcc
1741 tgacggctgg gacaacctga cgatgccgca cgcccagaag acgaaagcgg atttagcgaa
1801 gatgacgatc catgaatcgc atattcgcga tctctccgcc tgggaccaga ccgttccggc
1861 cgaactgcgc ggtaaatacc ttgcgctgac cgccggcgac agcaatatgg tgcagcatct
1921 gaagacgctc tccgcttcag gtgtgacgca cgttgagctg ctgccggtct tcgatctggc
1981 gacggtgaac gagttcagcg acaaggtcgc cgatattcag caaccgttca gtcgcctgtg
2041 tgaagtgaac agcgcggtga aaagcagcga gttcgccgga tactgcgaca gcggctccac
2101 ggtggaagag gtactgaatc agcttaagca gagcgatagc caggataatc cgcaggtgca
2161 ggcgctgaat acgctggtgg cgcagaccga ctcctacaac tggggctacg atccgttcca
2221 ctacacggtt ccggaaggat cctacgcgac cgatccggaa ggcacgacgc gcatcaaaga
2281 gttccgcacc atgattcagg cgatcaagca ggatctgggg atgaacgtca ttatggacgt
2341 ggtctacaac cacaccaacg ccgcgggtcc gaccgatcgc acgtcggtac tggataagat
2401 cgtaccctgg tactatcagc gtctgaatga aaccaccggc agcgtcgaat ccgccacctg
2461 ctgttccgac tccgcgccgg agcatcggat gttcgccaag ctgatcgccg actcgctggc
2521 ggtatggacc accgattata aaatcgatgg tttccgcttc gacctgatgg gctatcaccc
2581 gaaagcgcaa attctgtcgg cgtgggagcg cattaaggcc ctgaacccgg atatctactt
2641 cttcggcgaa ggctgggact ctaaccagag cgatcgtttt gaaatcgcct cgcagatcaa
2701 cctgaaaggt accggtatcg gcacgttctc cgatcgcctg cgcgattccg tgcgcggcgg
2761 cggcccgttc gattccggcg atgcgctgcg tcagaaccag ggcatcggca gcggtgcggg
2821 cgtgttgcca aacgaactcg ccagcctgag cgatgaccag gtccgccatc tggccgatct
2881 gacccgtctg gggatggccg gtaacctcgc tgactttgtg atgatcgaca aagacggcgc
2941 ggcgaagaaa ggcagcgaaa ttgactataa cggcgcgcct ggcggctacg ccgcagaccc
3001 gacggaagtc gtcaactatg tttcgaaaca cgacaaccag acgctgtggg acatgatcag
3061 ctacaaagcg tcccaggaag ccgatctggc gacccgcgtt cgcatgcagg ccgtttcgct
3121 ggcgacggtg atgctggggc aggggatcgc gttcgatcag cagggctctg aactgctgcg
3181 ttcgaaatcc tttacccgcg actcctacga ctccggcgac tggttcaacc gcgtggatta
3241 ttccctgcag gataataact acaacgttgg tatgccgcgc atcagcgatg acggcagcaa
3301 ctatgaggtg attactcgcg tcaaagagat ggttgccacc ccgggcgaag cggagctgaa
3361 gcagatgacc gcgttctacc aggagctgac cgagctgcgg aaatcctcgc cgttgttcac
3421 attgggcgat ggcagcgcgg taatgaagcg cgttgatttc cgtaataccg gttccgacca
3481 acaggccggt ctgctggtga tgaccgtgga tgacgggatg aaagcgggcg ccagcctgga
3541 tagccgcctt gacggtctgg tggtggcgat taacgccgcg ccggaaagcc gcacgctgaa
3601 tgagtttgcc ggtgaaacgc tgcagctgag cgcgatccag cagacggcgg gcgaaaactc
3661 gctggcaaac ggcgtgcaga ttgccgctga tggcaccgtc actctgccgg cctggtccgt
3721 tgcggtgctg gaactgccgc agggcgaagc ccagggcgcc gggctgccgg taagcagtaa
3781 gtaaaacctt aacctcccgg cccgcggcgt caaaaccccg cgccgggatg gcgtgtcttg
3841 attaaggaga agccggtgct tgttcgcccg caagagccgt acccgcagtc cgagcccccg
3901 gcggcggtag gaagaatggt gcagatcccc tacgtgaccg taccgctgta cgcgcgctgc
3961 tgattgcgct cggatggttc ggcggcgagc agtggcggaa caagcccgag ccgcagccga
4021 tgaggcagtc ggtagcccat gcggcgttag tcccgctgaa tcaaccggcg gtcaaagctg
4081 ccgtggcgcc g
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