GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS LACIS904 1245 bp ds-DNA BCT 27-MAY-1991
DEFINITION Lactococcus lactis insertion element IS904 coding for a transposase
ACCESSION X52273
KEYWORDS insertion element; insertion element IS904; transposon.
SOURCE Lactococcus lactis DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1245)
AUTHORS Rauch,P.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1245)
AUTHORS Rauch,P.J., Beerthuyzen,M.M. and de,V.W.M.
TITLE Nucleotide sequence of IS904 from lactococcus lactis subsp. lactis
strain NIZO R5
JOURNAL Nucleic Acids Res. 18, 4253-4254 (1990)
STANDARD full automatic
COMMENT *source: strain=lactis NIZO R5.
From EMBL entry LLIS904; dated 25-JUL-1990.
FEATURES Location/Qualifiers
repeat_unit 1..39
/note="imperfect inverted repeat A"
promoter 265..270
/note="put. -35 region (1)"
promoter 286..291
/note="put. -10 region (1)"
misc_feature 289..336
/note="dyad symmetry region"
promoter 302..307
/note="put. -35 region (2)"
promoter 326..331
/note="put. -10 region (2)"
CDS 444..1202
/note="put. transposase (AA 1-253)"
/codon_start=444
repeat_unit 1207..1245
/note="imperfect inverted repeat A'"
BASE COUNT 426 a 213 c 255 g 351 t
ORIGIN
1 tggaaaggtt ataataaact agacacaaag ttaagagaaa tcgtggaaag gtttattatg
61 ggaagaagaa aattcgataa acaatttaaa aattctgcag taaaactcat tcttgaagag
121 ggttactctg ttaaagaagt cagccaagag cttgaggttc atgccaatag tctttatcgc
181 tgggttcaag aagttgaaga atatggagaa agtgcttttc caggcaatgg gacagcccta
241 gctgatgccc aacataagat taaattgtta gagaaagaaa atcgttatct tcaggaggaa
301 cttgaacttc taaaaaagtt ccaggtcttc ttgaagcgaa gcaagtaaaa cgttttgaat
361 ttctcttgaa acatcatggg aagataaaaa ttaagcatgc agtaaaagtt cttaaggttt
421 ctcgctcagg tttctatgaa tacatgcatc gtcgtccttc aaaacaacaa gtggagagag
481 aaattctctc agagaagata aaagctgtct ttcatgagca taagggacgc tatggtgcgg
541 ttagaattac caaggtactt cataatactg gtattatgac caacacgaaa cgtgttggga
601 aactgatgca cttgatggga ctttatgcca agggaagccg atataaatat aaacattaca
661 acagaaaagg cagttcgctt tcaagaccca atttaattaa tcagatcttt aaagcaacag
721 ctcctaataa agtatggctg ggagacatga cctatatccc taccaaagaa ggtaccttat
781 acttagccgt gaatatcgac gttttttcac gtaagattgt aggctggtca atgtcttcac
841 ggatgcaaga taaactggtg agggattact tcttacaagc ttgtgggaaa gaacatcctc
901 agcctggctt gattgtccat actgatcaag ggagtcaata tacaagctct cgttatcaat
961 ctactcttcg tcaagtcggt gctcaatcta gcatgagtcg taaaggaaat ccctatgaca
1021 atgcaatgat ggagtctttt tataagacgc taaagaggga gcttattaat gatgctcatt
1081 ttgagacaag agctgaggct actcaagaaa tatttaaata cattgagacc tattacaata
1141 caaaaaggat gcattcaggt cttgattaca agtctccaaa agactttgaa aaatataatt
1201 cttaaattct cttaactccg tgtctagttt ttcgttgact ttcca
//