GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MSG32PG 1299 bp ds-DNA BCT 27-MAY-1991
DEFINITION Mycobacterium bovis gene for 32kDa protein
ACCESSION X53034
KEYWORDS 32 kd protein; fibronectin-binding protein; secreted protein.
SOURCE Mycobacterium bovis DNA.
ORGANISM Mycobacterium bovis
Prokaryota; Bacteria; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE 1 (bases 1 to 1299)
AUTHORS Content,J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS De,W.L., de,l.C.A., Ooms,J. and Content,J.
TITLE Nucleotide sequence of the 32kDa-protein gene (antigen 85 A) of
Mycobacterium Bovis BCG
JOURNAL Nucleic Acids Res. 18, 3995-3995 (1990)
STANDARD full staff_review
COMMENT *source: strain=BCG, Paris 1173P2; library=Lambda gt11; *source:
clone=bacteriophage M2,3; See also <M27016>.
From EMBL entry MB32PG; dated 14-JAN-1991.
FEATURES Location/Qualifiers
RBS 79..85
sig_peptide 91..219
CDS 91..1107
/product="32kDa protein"
/codon_start=91
mat_peptide 220..1104
/product="32kDa protein"
BASE COUNT 223 a 412 c 443 g 221 t
ORIGIN
1 actgccgggc ccagcgcctg cagtctgacc taattcagga tgcgcccaaa catgcatgga
61 tgcgttgaga tgaggatgag ggaagcaaga atgcagcttg ttgacagggt tcgtggcgcc
121 gtcacgggta tgtcgcgtcg actcgtggtc ggggccgtcg gcgcggccct agtgtcgggt
181 ctggtcggcg ccgtcggtgg cacggcgacc gcgggggcat tttcccggcc gggcttgccg
241 gtggagtacc tgcaggtgcc gtcgccgtcg atgggccgtg acatcaaggt ccaattccaa
301 agtggtggtg ccaactcgcc cgccctgtac ctgctcgacg gcctgcgcgc gcaggacgac
361 ttcagcggct gggacatcaa caccccggcg ttcgagtggt acgaccagtc gggcctgtcg
421 gtggtcatgc cggtgggtgg ccagtcaagc ttctactccg actggtacca gcccgcctgc
481 ggcaaggccg gttgccagac ttacaagtgg gagaccttcc tgaccagcga gctgccgggg
541 tggctgcagg ccaacaggca cgtcaagccc accggaagcg ccgtcgtcgg tctttcgatg
601 gctgcttctt cggcgctgac gctggcgatc tatcaccccc agcagttcgt ctacgcggga
661 gcgatgtcgg gcctgttgga cccctcccag gcgatgggtc ccaccctgat cggcctggcg
721 atgggtgacg ctggcggcta caaggcctcc gacatgtggg gcccgaagga ggacccggcg
781 tggcagcgca acgacccgct gttgaacgtc gggaagctga tcgccaacaa cacccgcgtc
841 tgggtgtact gcggcaacgg caagccgtcg gatctgggtg gcaacaacct gccggccaag
901 ttcctcgagg gcttcgtgcg gaccagcaac atcaagttcc aagacgccta caacgccggt
961 ggcggccaca acggcgtgtt cgacttcccg gacagcggta cgcacagctg ggagtactgg
1021 ggggcgcagc tcaacgctat gaagcccgac ctgcaacggg cactgggtgc cacgcccaac
1081 accgggcccg cgccccaggg cgcctagctc cgaacagaca caacatctag cggcggtgac
1141 ccttgtggtc gccgccgtag atgtttccta aatcccgtcc ctagctcccg ccgcgggccg
1201 tgtggttagc tacctgacgg gctaggggtt ggccggggcg gttgacgccg ggtgcacaca
1261 gcctacacga acggaaggtg gacacatgaa gggtcggtc
//