GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MBOLIPASE 1469 bp ds-DNA BCT 27-MAY-1991
DEFINITION Moraxella L1 gene for lipase 1 (EC 3.1.1.3)
ACCESSION X53053
KEYWORDS lipase; triacylglycerol lipase.
SOURCE Moraxella sp. DNA.
ORGANISM Moraxella sp.
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 1469)
AUTHORS Feller,G.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Feller,G., Thiry,M.E. and Gerday,C.
TITLE Sequence of a lipase gene from the Antarctic psychrotroph Moraxella
TA144
JOURNAL Nucleic Acids Res. 18, 6431-6431 (1990)
STANDARD full staff_review
COMMENT *source: strain=TA144; cell line=E.coli RR1; clone=pULG312;
*source: library=genomic in pSP73;
Data kindly reviewed (04-DEC-1990) by Feller G.
From EMBL entry MSLIPASE; dated 31-DEC-1990.
FEATURES Location/Qualifiers
promoter 306..311
/note="-35 region"
promoter 332..337
/note="-10 region"
CDS 432..1388
/note="lipase 1"
/codon_start=432
BASE COUNT 437 a 302 c 306 g 424 t
ORIGIN
1 gatatctgtt attggcttgg atgaaagacg agtgatacag ctaagggcta aaaatattac
61 gtagtaaatc tatagtcaat attatataag atgagaccat atctttaata aatatctatc
121 ataaagacaa gcaagttata ttatcacatc aaagtgccta ctcagtttta gatgtgttat
181 tggcgctaca ctaaagcatg tcctcaatta gcctgtttat acatgtagtc catttataca
241 tgcgctttct ttaaattttc actacagtcc agttgcattc gataccgtgc aggtgattta
301 tgactttgac aatgaaaaga ttaatgtcta ctattatttt gccttatcag gcatacagtc
361 tgaaataggc taaaacaaca taaatagctc atttaagaca atcatagcca cacgactctc
421 acataaaaag aatgtttatc atgataaaaa agtctgagct tgcaaaagct attatcgtca
481 cgggcgcgtt ggttttttct ataccgactt tagctgaagt taccttatct gaaaccacgg
541 ttagctcaat aaaatctgaa gctacggtta gctcaacaaa aaaagcatta ccagcaacgc
601 cgtcagattg tattgctgat agcaaaatta cagcagttgc tctttcagat actcgtgata
661 atggcccctt ttctatccgt accaaacgca tttcgcgcca gtcggcgaag ggtttcggtg
721 gtggtacgat tcactaccca accaacgcta gcggctgcgg tctgttaggc gctattgccg
781 tggttcctgg ttacgtttct tacgagaact ctatcaagtg gtggggaccg cgtctggctt
841 cttggggctt tgtcgttatc actatcaata ccaactctat ctatgatgat ccagatagta
901 gagccgcaca attgaatgca gcactagata acatgattgc tgatgacacc gtcggcagta
961 tgatagatcc taagcggttg ggcgctattg gctggtcaat gggtggcggc ggcgcgctta
1021 aactggcaac ggagcgcagc acagtacgag ccattatgcc actagcgccc tatcatgaca
1081 aaagctatgg cgaggtaaaa acgccaacct tggtcattgc ctgcgaggat gaccggattg
1141 ctgagactaa gaaatatgcc aacgcgtttt ataaaaatgc cattggcccc aagatgaagg
1201 ttgaagtaaa caatggcagt cacttttgtc caagctaccg ctttaacgag atacttttaa
1261 gtaagcctgg catcgcgtgg atgcagcgtt atattaataa tgatacacgc ttcgataaat
1321 tcttatgtgc caacgaaaac tatagcaaaa gccctcgtat ctcagcttat gattataaag
1381 attgcccata aagcggtgta taggaatggt gacattcgct ttaagtatgc ctttatttgc
1441 gtatattaga tttaagtaca ttcgaattc
//