GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MSGIS986 1378 bp ds-DNA BCT 27-MAY-1991
DEFINITION M. tuberculosis insertion sequence IS986
ACCESSION X52471
KEYWORDS IS3 family; insertion sequence; repetitive element; transposase.
SOURCE Mycobacterium tuberculosis DNA.
ORGANISM Mycobacterium tuberculosis
Prokaryota; Bacteria; Firmicutes; Mycobacteria; Mycobacteriaceae.
REFERENCE 1 (bases 1 to 1378)
AUTHORS Dale,J.B.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS McAdam,R.A., Hermans,P.W., van,S.D., Zainuddin,Z.F., Catty,D.,
van,E.J.D. and Dale,J.W.
TITLE Characterisation of a Mycobacterium tuberculosis insertion sequence
belonging to the IS3 family
JOURNAL Mol. Microbiol. 4, 1607-1613 (1990)
STANDARD full staff_review
COMMENT *source: isolate=50410; clone=A3. See also entry M29899.
From EMBL entry MTIS986; dated 12-OCT-1990.
FEATURES Location/Qualifiers
repeat_unit 11..40
/note="inverted repeat"
RBS 51..58
/note="put. ribosome binding site"
CDS 64..285
/note="ORFa1 (AA 1 - 74)"
/codon_start=64
RBS 203..206
/note="put. ribosome binding site"
CDS 210..386
/note="ORFa2 (AA 1 - 59)"
/codon_start=210
RBS 476..481
/note="put. ribosome binding site"
CDS 488..1321
/note="put. transposase (ORFb) (AA 1 - 278)"
/codon_start=488
CDS complement(632..1012)
/note="ORFc (AA 1 - 127)"
/codon_start=1012
repeat_unit 1339..1368
/note="inverted repeat"
BASE COUNT 272 a 447 c 436 g 223 t
ORIGIN
1 aggtcggcaa cctgaaccgc cccggcatgt ccggagactc cagttcttgg aaaggatggg
61 gtcatgtcag gtggttcatc gaggaggtac ccgccggagc tgcgtgagcg ggcggtgcgg
121 atggtcgcag agatccgcgg tcagcacgat tcggagtggg cagcgatcag tgagatcgcc
181 cgtctacttg gtgttgctgc gcggagacgg tgcgtaagtg ggtgcgccag gcgcaggtcg
241 atgccggcgc acggcccggg accacgaccg aagaatccgc tgagataaag cgcttgcggc
301 gggacaacgc cgaattgcga agggcgaacg cgattttaaa gaccgcgtcg gctttcttcg
361 cggccgagct cgaccggcca gcacgctaat tacccggttc atcgccgatc atcagggcca
421 ccgcgagggc cccgatggtt tgcggtgggg tgtcgagtcg atctgcacac agctgaccga
481 gctgggtgtg ccgatcgccc catcgaccta ctacgaccac atcaaccggg agcccagccg
541 ccgcgagctg cgcgatggcg aactcaagga gcacatcagc cgcgtccacg ccgccaacta
601 cggtgtttac ggtgcccgca aagtgtggct aaccctgaac cgtgagggca tcgaggtggc
661 cagatgcacc gtcgaacggc tgatgaccaa actcggcctg tccgggacca cccgcggcaa
721 agcccgcagg accacgatcg ctgatccggc cacagcccgt cccgccgatc tcgtccagcg
781 ccgcttcgga ccaccagcac ctaaccggct gtgggtagca gacctcacct atgtgtcgac
841 ctgggcaggg ttcgcctacg tggcctttgt caccgacgcc tacgctcgca ggatcctggg
901 ctggcgggtc gcttccacga tggccacctc catggtcctc gacgcgatcg agcaagccat
961 ctggacccgc caacaagaag gcgtactcga cctgaaagac gttatccacc atacggatag
1021 gggatctcag tacacatcga tccggttcag cgagcggctc gccgaggcag gcatccaacc
1081 gtcggtcgga gcggtcggaa gctcctatga caatgcacta gccgagacga tcaacggcct
1141 atacaagacc gagctgatca aacccggcaa gccctggcgg tccatcgagg atgtcgagtt
1201 ggccaccgcg cgctgggtcg actggttcaa ccatcgccgc ctctaccagt actgcggcga
1261 cgtcccgccg gtcgaactcg aggctgccta ctacgctcaa cgccagagac cagccgccgg
1321 ctgaggtctc agatcagaga gtctccggac tcaccggggc ggttcaggcc ccgatggt
//