GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS CDVCDTOX 1683 bp ds-DNA VRL 27-MAY-1991
DEFINITION Corynebacterium diphtheriae virus gene for diphtheria toxin
ACCESSION X00703
KEYWORDS signal peptide; toxin.
SOURCE Corynebacterium diphtheriae virus DNA.
ORGANISM Corynebacterium diphtheriae virus
Viridae; Nonclassified viruses.
REFERENCE 1 (bases 1 to 1683)
AUTHORS Collier,R.J. and Kaplan,D.A.
TITLE Immunotoxins
JOURNAL Sci. Am. 251, 44-52 (1984)
STANDARD full automatic
COMMENT SWISS-PROT; P00588; DTX$CORBE.
The hydrophobic region of the B chain is responsible for the
toxin's insertion into the endosomal membrane and therefore for the
A chain's emergence from an endosome into the cytoplasm. The
disulfide bond (S-S) between Cys(186) and Cys(201) links the the
two chains.
From EMBL entry NCCDTOX; dated 06-JUL-1989.
FEATURES Location/Qualifiers
CDS 1..75
/note="signal peptide"
/codon_start=1
CDS 76..654
/note="A chain"
/codon_start=76
misc_feature 516..518
/note="put. catalyticly active Glu"
misc_feature 631..633
/note="Cys(186) involved in S-S bond"
CDS 655..1680
/note="B chain"
/codon_start=655
misc_feature 673..676
/note="Cys(201) involved in S-S bond"
CDS 868..1155
/note="hydrophobic region"
/codon_start=868
misc_feature 1456..1458
/note="Cys(461) involved in S-S bond"
misc_feature 1486..1486
/note="Cys(471) involved in S-S bond"
BASE COUNT 510 a 313 c 404 g 456 t
ORIGIN
1 gtgagcagaa aactgtttgc gtcaatctta ataggggcgc tactggggat aggggcccca
61 ccttcagccc atgcaggcgc tgatgatgtt gttgattctt ctaaatcttt tgtgatggaa
121 aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca aaaaggtata
181 caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg gttttatagt
241 accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc gctctctgga
301 aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct cgcactaaaa
361 gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga accgttgatg
421 gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc gcgtgtagtg
481 ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa ctgggaacag
541 gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa acgtggccaa
601 gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag gcgatcagta
661 ggtagctcat tgtcatgcat aaatcttgat tgggatgtca taagggataa aactaagaca
721 aagatagagt ctttgaaaga gcatggccct atcaaaaata aaatgagcga aagtcccaat
781 aaaacagtat ctgaggaaaa agctaaacaa tacctagaag aatttcatca aacggcatta
841 gagcatcctg aattgtcaga acttaaaacc gttactggga ccaatcctgt attcgctggg
901 gctaactatg cggcgtgggc agtaaacgtt gcgcaagtta tcgatagcga aacagctgat
961 aatttggaaa agacaactgc tgctctttcg atacttcctg gtatcggtag cgtaatgggc
1021 attgcagacg gtgccgttca ccacaataca gaagagatag tggcacaatc aatagcttta
1081 tcgtctttaa tggttgctca agctattcca ttggtaggag agctagttga tattggtttc
1141 gctgcatata attttgtaga gagtattatc aatttatttc aagtagttca taattcgtat
1201 aatcgtcccg cgtattctcc ggggcataaa acgcaaccat ttcttcatga cgggtatgct
1261 gtcagttgga acactgttga agattcgata atccgaactg gttttcaagg ggagagtggg
1321 cacgacataa aaattactgc tgaaaatacc ccgcttccaa tcgcgggtgt cctactaccg
1381 actattcctg gaaagctgga cgttaataag tccaagactc atatttccgt aaatggtcgg
1441 aaaataagga tgcgttgcag agctatagac ggtgatgtaa ctttttgtcg ccctaaatct
1501 cctgtttatg ttggtaatgg tgtgcatgcg aatcttcacg tggcatttca cagaagcagc
1561 tcggagaaaa ttcattctaa tgaaatttcg tcggattcca taggcgttct tgggtaccag
1621 aaaacagtag atcacaccaa ggttaattct aagctatcgc tattttttga aatcaaaagc
1681 tga
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