GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS NOCMEA10D 150 bp ds-DNA BCT 27-MAY-1991
DEFINITION Nocardia mediterranei DNA with pMEA100 attachment site attB
ACCESSION X05990
KEYWORDS attachment site.
SOURCE Nocardia mediterranei DNA.
ORGANISM Nocardia mediterranei
Prokaryota; Bacteria; Firmicutes; Nocardioform actinomycetes.
REFERENCE 1 (bases 1 to 150)
AUTHORS Madon,J., Moretti,P. and Huetter,R.
TITLE Site-specific integration and excision of pMEA100 in Nocardia
mediterranei
JOURNAL Mol. Gen. Genet. 209, 257-264 (1987)
STANDARD full automatic
COMMENT *source: strain=LBG A3136; clone=(lambda)ME1;
Data kindly reviewed (03-MAR-1988) by Huetter R.
From EMBL entry NMMEA10D; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 1..150
/note="chromosomal DNA"
repeat_region 22..44
/note="direct repeat 1 (homology region 1)"
misc_feature 51..98
/note="pMEA100 attB sequence"
repeat_region 55..81
/note="direct repeat 2 (homology region 2)"
repeat_region 70..92
/note="direct repeat 1 (homology region 1)"
repeat_region 124..150
/note="direct repeat 2 (homology region 2)"
BASE COUNT 31 a 50 c 49 g 20 t
ORIGIN
1 cctggacaca agaaagccgc ctcgacccga ggtcaagacg gcttcttcga acggtggcca
61 gggccggggt cgaaccggcg accttccgct tttcaggcgg acgctcgtac caactgagct
121 acctggccgg gaacgccggc aaggagccga
//