GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS PDEAMI 659 bp ds-DNA BCT 27-MAY-1991
DEFINITION P. denitrificans AMI gene coding for amicyanin
ACCESSION X55665
KEYWORDS amicyanin; methylamine dehydrogenase.
SOURCE Paracoccus denitrificans DNA.
ORGANISM Paracoccus denitrificans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 659)
AUTHORS van,S.R.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS van,S.R.J., Wansell,C.W., Reijnders,W.N., Oltmann,L.F. and
Southamer,A.M.
TITLE Mutagenesis of the gene encoding amicyanin of Paracoccus
denitrificans and the resultant effect on methylamine oxidation
JOURNAL FEBS Lett. 275, 217-220 (1990)
STANDARD full staff_review
COMMENT *source: strain=NCIB 8944; library=pUC19; clone=pAMI1;
From EMBL entry PDAMI; dated 01-FEB-1991.
FEATURES Location/Qualifiers
mat_peptide <1..93
/product="methylamine dehydrogenase beta subunit"
prim_transcript <151..>546
/gene="AMI"
CDS <1..96
/product="methylamine dehydrogenase beta subunit"
/codon_start=1
repeat_unit 101..128
/rpt_type=inverted
RBS 141..145
sig_peptide 151..228
CDS 151..546
/product="amicyanin"
/gene="AMI"
/codon_start=151
mat_peptide 229..543
/product="amicyanin"
/gene="AMI"
misc_binding 502..522
/bound_moiety="copper"
CDS 564..>659
/note="ORF1"
/codon_start=564
BASE COUNT 128 a 223 c 198 g 110 t
ORIGIN
1 gaattcgcca acgacatcat ctggtgcttc ggcgccgagg acgatgccat gacctatcac
61 tgcacgatct cgcccatcgt gggcaaggcg agctgacggc ggccgggcgc gcatgcccgc
121 gcccggcctt catcccgcat ggagaagcaa atgatttctg cgaccaagat ccgctcgtgc
181 ctggcggcct gcgtcctggc ggcattcggc gcgacgggcg ccctggccga caaggcgacg
241 atcccctcgg aaagcccctt tgccgccgcc gaggtggccg atggcgccat cgtcgtcgac
301 atcgccaaga tgaaatacga aacccccgaa cttcatgtga aggtcggcga caccgtcacc
361 tggatcaacc gcgaggcgat gccgcacaat gtccatttcg tcgccggcgt gctgggcgag
421 gcggcgttga aaggcccgat gatgaagaag gagcaggcct attccctgac cttcaccgag
481 gccggcacct atgactatca ctgcaccccg catcccttca tgcgcggcaa ggtcgtcgtc
541 gagtagcatg tgaaaggccg gccatgtgga ttccctacga cattcgcggc tcgctgaagc
601 ctgaatcgcc agccgggacg atccgcctgt cgcgaaccga taccagcccg cgcgaattc
//