GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS PSECELB 2144 bp ds-DNA BCT 27-MAY-1991
DEFINITION Pseudomonas fluorescens celB gene for endoglucanase B
ACCESSION X52615
KEYWORDS celB gene; endoglucanase; endoglucanase B.
SOURCE Pseudomonas fluorescens DNA.
ORGANISM Pseudomonas fluorescens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2144)
AUTHORS Gilbert,H.J.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2144)
AUTHORS Gilbert,H.J., Hall,J., Hazlewood,G.P. and Ferreira,L.M.
TITLE The N-terminal region of an endoglucanase from Pseudomonas
fluorescens subspecies cellulosa constitutes a cellulose- binding
domain that is distinct from the catalytic centre.
JOURNAL Mol. Microbiol. 4, 759-767 (1990)
STANDARD full automatic
COMMENT *source: strain=sub species cellulosa;
From EMBL entry PFCELB; dated 30-JUL-1990.
FEATURES Location/Qualifiers
CDS 301..1833
/note="precursor protein (AA -29 to 482)"
/codon_start=301
CDS 301..387
/note="signal peptide (AA -29 to -1)"
/codon_start=301
CDS 388..1833
/note="endoglucanase B (AA 1-482)"
/codon_start=388
BASE COUNT 477 a 542 c 577 g 548 t
ORIGIN
1 ttctcgacgc ttaacgaacg cattcgcttt gagtggccat taaacgctgg gtgcggagcc
61 ggatacgtca cagaaagagt gagccttttc caatcaggac ttttttgcgc taggctatca
121 cccacaacaa taaagtattg gctcgctctc acgaaatacc catcaacccg tagtgcatag
181 cttgtttatg gatggcattt tttccatagg ctgctgcacg cgcatcgtct gaaaacacct
241 gcatattatt gtgggggtgt tttttacatc ggctggtatt aacaaaagag gaataacacg
301 atgaacttgc tatcaggttg ggtaagacct ctgatgctgg ggtgtggttt gctcggagcc
361 gcattatcgg cggggagcat acaggcagca gtgtgtgaat atcgggtgac gaatgaatgg
421 ggcagtggtt ttaccgccag tattcgaatc accaacaatg gcagcagtac gattaacggt
481 tggagtgtca gttggaatta caccgatggc tcccgcgtta ccagtagctg gaatgcggga
541 ctgagtggtg ccaatcccta tagtgcgaca ccggtgggtt ggaatacctc tattccgatc
601 gggtcgagtg tggaatttgg agtgcagggc aacaatggca gcagtcgcgc acaagtgccg
661 gcagtgaccg gggcgatttg cggtggacaa ggttccagtg caccgtccag tgttgcgtct
721 tcatcttcat ccagcagtgt tgtttccagt actccccgct ccagctcatc gtcagtgtcg
781 agttctgtac ctggcacttc gtcgagcagt tccagctctg tgttaaccgg ggcccaggcg
841 tgtaactggt atggcacact gaccccttta tgcaacaaca ccagcaatgg ttggggctat
901 gaggatggtc gcagttgtgt ggcgcgaacc acctgtagtg cgcaaccagc gccttacggc
961 atagtatcga cctcatccag tacgccgcta tcctcaagca gctcatcgcg ctcctcggtt
1021 gccagcagtt cctccctcag tagtgcaacc tccagcagtg catcgtcagt gagcagcgtc
1081 cctcctattg acggtggctg taatggctat gccacacgct attgggattg ctgcaagccg
1141 cactgtggct ggagtgccaa tgtgccctcg ctggtatcgc ccctgcaaag ttgttcggcc
1201 aacaataccc gtttgagtga tgtgagtgtc ggcagcagtt gcgatggcgg tggcggttat
1261 atgtgttggg acaagattcc ctttgcggtc agtccaacac tggcttacgg ttacgcggcg
1321 acctccagtg gggatgtgtg cggtcgctgt tatcaattgc aatttaccgg cagttcctac
1381 aatgcgccgg gcgatcctgg ttcagcggca ctggcgggaa aaaccatgat cgtgcaggcc
1441 accaatattg gctacgatgt ttccggtggc cagtttgaca ttctggttcc cggcggtggt
1501 gttggagcct tcaatgcctg ctcggcgcaa tggggtgttt ccaatgcgga gttgggcgcg
1561 caatacggtg gatttctggc tgcctgtaag cagcagttgg gttacaacgc cagcctgtcg
1621 caatacaaaa gctgtgtgct caatcgctgt gacagtgtgt ttggttcgcg cggcctgacc
1681 caattgcaac aggggtgtac ctggtttgcc gagtggtttg aagcggcgga caatccttca
1741 ctcaaataca aagaagttcc ctgccctgca gagttaacaa cccgctccgg aatgaatcgc
1801 agtatcctga atgatattcg caacacctgt ccctgagtga ttacacagga tagaaacggc
1861 ccttttgtgg cagtttctca ttttaagtac acaggagccg atcaatagag aaaggtatat
1921 caactccata gcctatcctt atcaacccgc ctaaaaaata ctcatcgctt atgttttata
1981 aattttgatt atgagaaccg cggtgtcatt caattagccc tgattcctcc aactccctaa
2041 tctgtgcccc gtcgaaactt tctctggcga cattaaccac agaccttcag gagttacatt
2101 catggcaagt atcaattcgg aaattaaacc tttcagtgcc cagg
//