GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS VIBHLYC 2915 bp ds-DNA BCT 27-MAY-1991
DEFINITION Vibrio cholerae hlyB gene for haemolysin releasing protein
ACCESSION X16945
KEYWORDS haemolysin; hlyB gene; hlyC gene; unidentified reading frame.
SOURCE Vibrio cholerae DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2915)
AUTHORS Manning,P.A.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Alm,R.A. and Manning,P.A.
TITLE Characterization of the hlyB gene and its role in the production of
the EI Tor haemolysin of Vibrio cholerae 01
JOURNAL Mol. Microbiol. 4, 413-425 (1990)
STANDARD full staff_review
COMMENT SWISS-PROT; P15492; HLYB$VIBCH. SWISS-PROT; P15493; YHLB$VIBCH.
*source: strain=017; clone=pPM431; see <Y00557> for hly gene for
haemolysin.
Data kindly reviewed (13-MAR-1990) by Manning P.A.
From EMBL entry VCHLYB; dated 19-SEP-1990.
FEATURES Location/Qualifiers
promoter 73..78
/note="pot.-35 region"
promoter 92..97
/note="pot.-10 region"
RBS 124..128
/note="ribosome binding site"
CDS 135..1778
/note="haemolysin releasing protein (AA 1-548)"
/codon_start=135
terminator 1847..1868
/note="pot.stem-loop structure"
CDS 2048..2560
/note="unidentified reading frame (AA 1-271)"
/codon_start=2048
BASE COUNT 775 a 682 c 730 g 728 t
ORIGIN
1 gaattccctt tcacattagg gtaaaagtac gcaatcacaa tactaagcgc gagacagcgt
61 aagtccagtt tgctgacatc cctacaaccc atataggtca gcagactctt ttttctgaac
121 gataggtacg agacatgatc atcaataaat tttcccttaa atggatgttg gctattgccg
181 tcgccatccc tgcgatacga ctgttgtttg tggctttcac cagtctaaac accatgtcag
241 tgatgcaagc gcagtccaac agcttgtatg ccaacacggc tgcaccaatg cgtgccatgg
301 ctgaagcaac ctcacgtatt cctcggatgc gtgtcggtat cgatatgatg ctactgcaag
361 aaacggcgct caaagatgcg aaaggggtcc tcaaacgagt cgaagaggca agaaccgaag
421 atatcccaga aatgcgtcaa cgaatgcaag ttgcggttga ttctcaggtt aatccggaac
481 tcaaagagca ggcacgcaaa cttcaagctc gttttgaaca aatggtacgt gaagagttag
541 agcctatgct gcaagccttc gccaataacg atatgaccac ggcacaaaac atttaccgcg
601 ataaatacgc gccgacctat ggtgaaatgc gtaaacaagc caaccagatc ctcgatacgc
661 ttttgcagca agcggacagg caaaaccatg ccagtgtgga aagcttcgaa gcaggacgca
721 ccaagcaaat ggtgatcatt gcagcaggct tgatcatttc attcatcact tcactggtta
781 tcataacgaa cttacgtagc cgagtggctt acctgaaaga tcgtatgagt tctgcggcgg
841 cgaatctttc actgcgtact cgattggagt tggatggtaa cgatgaactg tgtgacatcg
901 gtaaaagctt caatgcgttc attgataaag tgcatcactc gattgaagaa gtggcagaaa
961 actcaaaaga gctggcgacg atggcctcta gtgtgtcgca gcgcgcgcac atgacgcaat
1021 ctaactgtgc ttcgcagcga gatagaacag tgcaagttgc gacggcgatt catgagcttg
1081 gtgccaccgt atccgaaatc gcttccaatg cggccatggc tcgcgatgtc gcgaacgaag
1141 cgacgctgca ttctggtgaa gggaaaaaag tggtaggcga agtgcaaaat cggatccaaa
1201 cactggtcaa tgaactcgat aatgccactc aagttgtctc atcactggcg acccaaatta
1261 acggtattag ctcaacactt gataccattc gcagtatttc tgagcaaacg aacctattgg
1321 cgctcaacgc tgcgattgaa gctgcgcgag cgggtgaaca aggtcgtggt tttgcggtgg
1381 tggcggatga agttcgcaca ttagcaagtc gttcagcggc atcgacggaa gagatccagc
1441 aagtcattaa tcgccttcaa acggagtcaa ctcgcgcagt agaagcaatg gaaaaaggtc
1501 gctcgcaaag tgatgtggtg gttgagtttt ccgctaaagc gaaccaatct ctcacagaga
1561 tcaacagcca aattgatcag attaatgatc aaaatattca agttgcgacc gcgacagagg
1621 aacaatcaac cgtggtggaa gacattaatc gcaacgttga agacatcaac caactgacga
1681 cagaaacctc gcatgttgcg gatgagttaa gccgagccag tgcaagcttg caacgtctct
1741 cttcgcaact ggataaactg gtgggcagtt ttgaacttta accaatgact acgctaagca
1801 agcattcatg tcgcttgctg atgacgttga tattctattt ctcactaagc ggcctttcta
1861 ggccgctttt ttatcgctct tcgccttttt tatgttgcct caaccaaagt tggggttgta
1921 cacattttgt gttaatgaaa tgttattaat tttatgtttt gttcacattt tgaccttttg
1981 agattcggaa atccgccact tctcttctag cttaattaac ggtaagaggt ctgacttcta
2041 ccgagtaatg accctgtttt cagttaatga gactcacgcc acttcgtgtg gttccaagac
2101 tgaagacaag aacgagaagg aatatccctt gaataaaatc attatattaa tcgctctctc
2161 actgttttct tcctcaatct gggccggaac cagtcgtcac gcgttatcgc aacaaggcta
2221 tacccaaacc cgttatccaa ttgtcttagt tcatggccta tttggcttcg acaccttggc
2281 ggggatggat tactttcacg gcattccaca atcactgacc cgagatggcg ctcaagtgta
2341 tgtggcacag gtttcagcga ccaacagctc cgagcgccga ggtgagcagt tattggctca
2401 ggtggaatcg ctactggcgg taaccggagc gaaaaaagtg aacttgattg ggcatagtca
2461 tggtggcccc accattcgct atgtggcatc agtgcgtccg gatttagtcg cctcagtgac
2521 cagtattggc ggggtgcata aaggctctgc gttgccgact tagtgcgtgg tgtgattcca
2581 tcgggttccg tgagtgagca ggtagcggtt gggttaaccc aaggcttggt ggctttgatt
2641 gatctgctct ctggcggaaa agcgcatcca caagatcccc ttgcttcgct ggcggcgctc
2701 accaccgaag gatcgctgaa attcaatcag tattatcctg aaggtgttcc cacatcagcg
2761 tgtggagagg gggcgtacca agtgaatggg gtacgttact actcatggag tggcgctgcg
2821 accgtcacca atattcttga tccaagcgat gtggcgatgg ggttgattgg gttggtcttt
2881 aatgagccga atgatggctt ggtcgcaacc tgcag
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