GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS VIBOMPW 1031 bp ds-DNA BCT 27-MAY-1991
DEFINITION Vibrio cholerae ompW gene for outer membrane protein
ACCESSION X51948
KEYWORDS outer membrane protein.
SOURCE Vibrio cholerae DNA.
ORGANISM Vibrio cholerae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1031)
AUTHORS Manning,P.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1031)
AUTHORS Jalajakumari,M.B. and Manning,P.A.
TITLE Nucleotide sequence of the gene, ompW, encoding a 22kDa immunogenic
outer membrane protein of Vibrio cholerae
JOURNAL Nucleic Acids Res. 18, 2180-2180 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17266; OMPW$VIBCH.
Data kindly reviewed (15-JUN-1990) by Manning P.A.
From EMBL entry VCOMPW; dated 22-JUN-1990.
FEATURES Location/Qualifiers
promoter 102..107
/note="put. -35 region"
promoter 126..131
/note="put. -10 region"
RBS 149..154
/note="put. ribosome binding site"
CDS 161..808
/note="precursor protein (AA -20 to 196)"
/codon_start=161
CDS 161..220
/note="signal peptide (AA -20 to -1)"
/codon_start=161
CDS 221..808
/note="mature outer membrane protein (AA 1-196)"
/codon_start=221
BASE COUNT 290 a 202 c 214 g 325 t
ORIGIN
1 agagtattga agatgttgca aaagtgtagc aagttgcaaa cacattatta atgtgcctaa
61 atgtagcaaa ttgatttcct acaagtttgt gtgatttttg tgtgctactg tgcgcgcaac
121 acaaagataa caacatagcc ctacaaaaaa ggaaaacgtc atgaaacaaa ccatttgcct
181 agccgtactt gcagccctac tagccgctcc tgtatttgct caccaagaag gtgactttat
241 tgtgcgcgcg ggtattgcct cggtagtacc taatgacagt agcgataaag tgttaaacac
301 tcaaagtgag ttggcagtta atagcaatac ccacttaggg ttaacgcttg gctatatgtt
361 tactgacaac atcagttttg aagtcctcgc tcgtacgcca ttttcacata agatttctac
421 ctctggtggt gagttaggta gccttggtga tattggtgaa acaaaacatt tgccacctac
481 ctttatggtc caatactact ttggtgaagc taattcgaca aaccgtccat atgttggtgc
541 gggtttgaat tacaccactt tctttgatga aagctttaat agtacgggta ctaataatgc
601 attgagtgat ttaaaactgg acgactcatg gggacttgct gctaacgttg gctttgatta
661 tatgctcaat gatagctggt tcctcaacgc ttatgtgtgg tatgccaata ttgaaacaac
721 ggcaacctac aaagcaggtg cagatgccaa atccacggat gttgaaatca atccttgggt
781 atttatcatc gcgggtggtt ataagttcta acgccctatt tcgaaaataa agccgcgaaa
841 tcgctttatt tttttgtggc ctcgatttca tcatttttag cgtagtgatt gtttctaacg
901 taatctctat tcagagcctg agacgtacgc taatccctgt tgatactgat aaataggtgt
961 caatttaagg cgcaaccata tcgtaagatt tatctttatg atttctttct tattctggct
1021 tcagatcagg t
//