GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS WOLFRD 5758 bp ds-DNA BCT 27-MAY-1991
DEFINITION Wolinella succinogenes frdA, frdB, and frdC genes endcoding
subunits of fumarate reductase (EC 1.3.99.-)
ACCESSION X51509
KEYWORDS Fe/S protein; cytochrome b; diheme cytochrome b;
electron transfer flavoprotein; electron transport; flavoprotein;
frdA gene; frdB gene; frdC gene; fumarate reductase;
fumarate reductase flavoprotein subunit;
fumarate reductase iron-sulfur subunit; iron-sulfur flavoprotein.
SOURCE Wolinella succinogenes DNA.
ORGANISM Wolinella succinogenes
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Anaerobic gram-negative straight, curved and helical rods.
REFERENCE 1 (bases 1 to 5758)
AUTHORS Kroeger,A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 3 to 1522)
AUTHORS Koertner,C., Lauterbach,F., Tripier,D., Unden,G. and Kroeger,A.
TITLE Wolinella succinogenes fumarate reductase contains a dihaem
cytochrome b
JOURNAL Mol. Microbiol. 4, 855-860 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17412; FRDA$WOLSU. SWISS-PROT; P17413; FRDC$WOLSU.
SWISS-PROT; P17596; FRDB$WOLSU.
From EMBL entry WSFRD; dated 04-SEP-1990.
FEATURES Location/Qualifiers
promoter 588..593
/note="-35 region"
promoter 611..616
/note="-10 region"
precursor_RNA 622..4212
/note="primary transcript"
CDS 689..1456
/note="flavoprotein (AA 1-256)"
/codon_start=689
CDS 1481..3448
/note="Fe/S protein (AA 1-656)"
/codon_start=1481
CDS 3451..4167
/note="diheme-cytochrome b (AA 1-239)"
/codon_start=3451
terminator 4179..4200
/note="terminator"
BASE COUNT 1589 a 1389 c 1305 g 1475 t
ORIGIN
1 ggcgaggcgg ttttggcggc ttatgatgag attgagatga aagaggagga gaaagcgaag
61 ctcttgaccc aaaaattctc cacctttttc accgaaaaag gaatcaaccc ctttgtggtg
121 atcaaagagg gtgagcccgt ggagatggtg cttgaagagg cgaaagatta caatctactc
181 atcattggct ccagcgaaaa ctcattctta aataaaattt ttgcctctca tcaggatgat
241 tttattcaaa aagctccgat tcccgtcctc attgtcaaat aaaaaggggt ttggacgggg
301 ttttgtattt tattaaatat taaaaactac cgttcaaact ctaaaaaccg cgataaaaat
361 ttttactctt acagttccaa actaccttga tctctttaat tttcgattcc atttttcaag
421 actttgttta agttttaaat aagtttattt tttaccaaaa gtatcatcta tccctcaaaa
481 agccccgaca aaaggctgtt tgtcaaaaat gaaacacccc tctaaaacgt cacttttttt
541 gtcactaaaa gttacaataa attccaagca cccccaaatt aacgatatta acaaaggagt
601 caaaacgcgg tatgattcgg cgtgttaaga gcttgagagc tttccattgg ctcttagcag
661 aaactccaat actgcacagg ggaaacagat gacaaacgag agcatacttg agagttactc
721 cggtgtaact ccggaaagaa aaaaaagcag gatgcctgcg aagttggatt ggtggcagag
781 tgcgaccggt ctttttttgg gtctattcat gattggacat atgttctttg tctccacgat
841 tttgcttggc gacaatgtaa tgctatgggt caccaagaaa tttgagctag acttcatctt
901 tgagggtggc aaacccattg ttgtctcctt ccttgccgct tttgtttttg ccgtgtttat
961 cgcgcacgct ttccttgcaa tgagaaagtt cccgatcaac tatcgacaat acctcacctt
1021 taaaacacac aaagacctca tgagacacgg agacaccaca ctttggtgga ttcaagccat
1081 gacaggtttt gcgatgttct tccttggaag cgttcacctc tatatcatga tgacgcaacc
1141 ccaaaccatt ggacccgtga gctcctcttt ccgtatggtg agcgagtgga tgtggccttt
1201 gtatcttgtt cttctttttg ccgttgagct tcatggctct gtaggtctct acagactcgc
1261 ggtcaagtgg ggatggtttg atggagagac tcctgataaa actcgagcca atcttaaaaa
1321 actcaagact cttatgagcg ctttcttgat cgtccttggc ttgctcacat ttggtgctta
1381 tgtgaaaaaa ggcttggagc agactgatcc gaatattgac tacaaatatt tcgattacaa
1441 acgcacccac catcgttaat agcaaagagg aaggataaat atgaaagtac aatactgcga
1501 ttcgctggtt attggaggcg ggttagctgg tcttagagcc gctgtcgcca ctcaacaaaa
1561 aggcttgagc accatcgtgc ttagtttgat tcctgtcaag cgatcccact ccgctgccgc
1621 tcaagggggt atgcaagcca gccttggaaa ctccaagatg agtgatggag acaatgaaga
1681 tcttcacttt atggacaccg tcaaaggaag tgactggggc tgtgaccaaa aagtcgctcg
1741 aatgtttgtc aacactgcgc ccaaggctat cagagagtta gccgcctggg gggttccctg
1801 gacccgtatt cacaagggtg atcgaatggc gatcatcaat gcccaaaaga ccactatcac
1861 cgaagaggat tttagacatg gtctcatcca ctctcgagat tttgggggca ctaaaaaatg
1921 gagaacctgc tataccgccg atgctacggg tcacaccatg ctctttgcgg ttgccaatga
1981 gtgcttgaaa ctcggcgtct ctatccaaga tcgaaaagag gctattgccc ttattcatca
2041 agatggcaag tgctatggtg cggtcgtaag agaccttgtc acgggcgata tcattgctta
2101 tgtagccaaa ggaaccctca ttgctacagg gggctatgga cgaatctaca aaaacaccac
2161 taacgcagtg gtttgcgagg gcacaggaac ggctatcgct ctagagacag gaatcgctca
2221 gcttggaaat atggaagcgg ttcaattcca ccccacccct cttttccctt cagggattct
2281 attgaccgag ggttgtcgag gggatggagg tattctccga cgatgtggat ggacaccgat
2341 tcatgctgat tatgagcctg agaaaaaaga gctcgctagc cgtgacgttg tctctagacg
2401 aatgattgag cacatccgaa aaggcaaggg cgtacaatca ccctatggac agcacctttg
2461 gcttgacatc tccatcttgg gtcgaaaaca tatcgaaacc aacctccgag atgttcaaga
2521 gatctgcgaa tacttcgctg gaattgatcc tgcggagaag tgggcgcctg ttcttcctat
2581 gcaacactac tctatgggtg gaatccgaac tgactacaga ggcgaagcga aacttaaagg
2641 tctattctct gcgggtgaag ccgcttgctg ggatatgcac ggcttcaacc gactaggcgg
2701 aaactccgtc agcgaagccg tcgtagcggg tatgatcgta ggagagtatt tcgcagaaca
2761 ttgcgccaac actcaagtgg atcttgaaac taagacttta gagaagtttg tgaaaggcca
2821 agaagcctac atgaaaagcc tcgtggagag caaaggcacc gaggatgtat tcaagattaa
2881 aaatcgcatg aaagatgtaa tggatgacaa cgtgggaatc ttccgcgatg gtccgcacct
2941 agaaaaatcg gtcaaagagc ttgaagaact ctacaaaaaa tccaaaaatg taggaatcaa
3001 aaacaagcga ctccacgcca accctgagct cgaagaggct taccgtgtgc ctatgatgct
3061 caaagtcgct ctttgcgttg ccaaaggcgc tctagatcga acagagagcc gaggagctca
3121 caatcgtgag gattacccca agcgagacga tattaactgg ctcaaccgaa ctcttgcctc
3181 ttggcctaac cctgagcaga ctctccctac tctagagtat gaagctcttg atgttaatga
3241 gatggagatt gctcctagat atcgaggata tggagccaaa gggaactaca tcgagaatcc
3301 tttaagcgtg aaacgacaag aggagatcga caagattcag agcgaattgg aagcagctgg
3361 caaagatcga catgctattc aagaagctct catgccttat gagctacccg ctaaatacaa
3421 agccagaaac gaacgcctag gagacaaata atgggaagaa tgctaaccat cagggttttt
3481 aaatatgacc cccaaagtgc ggtttctaag ccccactttc aagaatacaa aattgaagaa
3541 gcgccctcga tgacgatttt tatcgtcctc aacatgattc gcgagaccta cgatcccgat
3601 ttgaacttcg acttcgtttg ccgcgcagga atttgcggaa gctgcggcat gatgatcaat
3661 ggtcgtccaa gcctagcttg ccgaaccttg actaaagatt ttgaggatgg cgtgatcacg
3721 cttcttcctt tgcccgcttt caaactcatc aaagacctct ccgtggatac aggaaactgg
3781 ttcaacggta tgagtcagcg agtggagagt tggattcacg ctcaaaaaga gcatgacatc
3841 tccaagcttg aggaaagaat cgagcccgaa gttgcccaag aggttttcga gcttgaccga
3901 tgtatcgaat gcggctgctg catcgcagct tgcggaacta agatcatgcg agaagacttt
3961 gtcggcgcgg ctggactcaa ccgtgtcgtt cgctttatga ttgatccaca cgatgagcga
4021 accgatgaag actactatga actcatcgga gatgatgatg gagtctttgg ttgtatgaca
4081 cttttggctt gccacgatgt ctgccccaaa aacctcccct tgcaaagcaa gatcgcttac
4141 cttcgaagaa aaatggtcag cgtcaactaa atctccttgg agcggggtct cccccgctcc
4201 tttatactcc ttgaagcctt caaactaatc atccattcta agcctttttc cccgccctgc
4261 cctctccttg agtgtcacac tttgacacat attcctagag aatcccctgt taaaggtgat
4321 cgttaattaa atatcacact cttaaaagat tccactttgt aacattttat ccatcaagaa
4381 tgccctcagg actaattcac aaaaaccccc aaacccgtta tgattggcat gctggtctat
4441 cttgcacaaa ctctgtgcga aggggggctg ccttgggtga cctctttggt gacccttgga
4501 gtcttggtgc tctttcttgc tcatggattt cttgccatgc gcaaacttcc tgccaactac
4561 cttcagctcg ctcagttcct ctcccatcgt cagcttctct ctcacacagg gacgacccta
4621 tggtggctac agtgcctcag cggtttagcg cttcttgtgt taggcggagc tcatctgctt
4681 atgattctca cctccaaaga gacgctcagc gctaccacgg cagcctaccg ctttgttcat
4741 gggggcctag gctctttcta tcttttgttg ctccttgtga gcactctaca cgcagggatt
4801 ggagcctatc gactgatctt aaagtggtgc cccattgagg caagcgagcc caaaacactc
4861 caaagaagaa ttcgcaatgt tcgatccgaa gtctttgggg tatttggagt attgacactc
4921 ttggcgcttt gggcagattt cacctatatc aaacatggaa aaaacctctc tccactctcc
4981 atccaagaga gactcacttt cgccaaaacc aaataagcct atccaagatt agaggaatct
5041 tttcctctaa tcaaaggcct tatatccaag caaaagacac ttgtcgtatc gaggcaagac
5101 gcaaattgca aaaaatcctc cccctattcc aatatctttg agcgtctcat agtaacgaat
5161 atcgaatcga agattcgcat tttggtcgtc agaaagggcg actccacccg tctcattgaa
5221 atagatgaag cgctcaatcc tcccacgaat catcaccaac gagccctgaa gcctctcctt
5281 ttcactctct ttaagaatct caatataggc ctcatgggag aggagcgaac gccctctgaa
5341 ggtgacataa tctttttgaa gggattgctg atggacttga agatagaaat cttccagcga
5401 ggtcgcctcc aaagtggcgc tcaccagaag agagaggagg attttagagg ttttcgacaa
5461 tcttagcaaa accgcgaaga tagatctctt gaagtgtttg caaaggaagc gaaagggaat
5521 caatctctag attctctcca cccacgcttc caatcacacc aagcgctaca cccccttttt
5581 gggcgagcga ttcaagggca gctagatgtt cgctagagac ctcaagcact gcgcgcgagg
5641 ggctctcact aaagagatcg acccctttga gcgcggtttt gactttgcac cctttttgac
5701 ccagcacgct cattttgcca agggctatag ccaatccccc gacattcaca tctttggc
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