GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS HAMDHFRORI 6157 bp ds-DNA ROD 27-MAY-1991
DEFINITION C. griseus dhfr origin of replication
ACCESSION X52034
KEYWORDS bent DNA; origin of replication; protein binding site.
SOURCE Cricetulus griseus DNA.
ORGANISM Cricetulus griseus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Cricetidae; Cricetinae;
Cricetini.
REFERENCE 1 (bases 1 to 6157)
AUTHORS Heintz,N.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Caddle,M.S., Lussier,R.H. and Heintz,N.H.
TITLE Intramolecular DNA triplexes, bent DNA and DNA unwinding elements
in the initiation region of an amplified dihydrofolate reductase
replicon
JOURNAL J. Mol. Biol. 211, 19-33 (1990)
STANDARD full staff_review
COMMENT *source: cell line=CHOC 400
From EMBL entry CGDHFRORI; dated 23-DEC-1990.
FEATURES Location/Qualifiers
rep_origin 2660..3550
/note="bidirectional"
protein_bind 2734..2750
/bound_moiety="RIP60"
repeat_region 3084..3245
/note="ORR-1; origin-region repeat 1"
protein_bind 3087..3095
/bound_moiety="OTF1/NF-III"
protein_bind 3394..3402
/bound_moiety="OTF1/NF-III"
misc_feature 3412..3460
/note="bent DNA; elements B1-B5"
protein_bind 3436..3443
/bound_moiety="AP1"
protein_bind 3453..3472
/bound_moiety="RIP60"
misc_feature 5734..5921
/note="Z-triplex DNA region"
BASE COUNT 1938 a 1172 c 1328 g 1719 t
ORIGIN
1 tctagaaaag aacactagct atctcatgta atcaaattgg tgactatcct aattattatg
61 agagagcttc cgtccagtaa gtgctagaag tagatgagag taccacagac aagcactgag
121 ccaagctcca ggagtcctgt tgaaaagaga gaggaaggat tgtaggagcc aaagagtcaa
181 gagcatgaca gggaaaccca cagagacagc tgacctgggc ttgtgggtgg gagctcatgg
241 actcttgacc aacaattagg gaactgcatg aggccaacct aggaactctg catgtgtgtg
301 acagttgtat agcatggtct gtttgtgagg cttttagcag tgggacaggg cctgtccttg
361 gcgcttgagt ggcttttggg aactgttcgc atgctggatt acaaccagct tgatgctggg
421 aagcattgtc tgctaatgat gcgcttgcat tgttggatct catggaggac tgcccttctg
481 aaagacagga gaagtgaata ggggagggga ttgggaggag aggaaggaga ggaaactgtg
541 atagggatgt aaaataaatt aaaaaattaa ttaattaaaa aagaacactt gtactggtag
601 attggctaaa atgaaacaaa gataaaagta cacaggaaaa agagaggaga aacctgggga
661 ggggggctcc aaagagaggt gaggggggga tgggaatggc agcttagtgg aggaaggaag
721 acatgaccta cacgaatcga gctgtagttt ttatctggag catagggtaa agatgtttga
781 ggagaaggag gaacacatgc ttgtaaaaca ggtcttcaga accagcaaca atcatacaga
841 tgtgtccagg gcgtccatgg gcagcatgaa gcgacagacc caacacatat ttaacagtcc
901 atacagtgtc ctatatgtgg tattgtaaag tgatgagtaa attgtcacta ggggactgtt
961 aatttagctg taaaggactt gtctggcatg tgggtattct tgggttccct ccttagcact
1021 gaaaaaaaaa aaaaacacac acacacacac acatatattc tagtgttttg tagaaaggat
1081 tcaagaaagc atgatttctc ttgataatca gataatgtaa taagacacac agtggttgat
1141 ttcagcaatc aagtacacct tgcctgtctg tttgttgtat gggatggttg ggtggttgtt
1201 tgtttggttt gtaagatggg ttggtgggtt ggtgggtggt tgcttggttg ggtagttggt
1261 tgggtgattg ggtgggtggg ttggttgggt gggtgggttg gttggtcgtt tggttgggtg
1321 gggtgggttt tgttttgaga cgagggattt actctatatc tcagtttgtc tcaaactcac
1381 tatgtccaca tgagtatgtg atgatgagat tatctaagac catagtgtct gtgttcatgg
1441 aatgctctct ctagcttaga gaatttaaaa aatggccatg tagggaacac ccctcagaaa
1501 aggagtttct atggcctcca agaataagaa tggatcctcc tagctcggag tcagcaagga
1561 acttgaagcc cttaatttta tagacacaaa ggaacccatt gtgtggctcc ttcccagcca
1621 agtttcagat gatgtcacag acctgcatgg caccttatgc agtcttttga ggtccaagaa
1681 taggatgcag ataagccatg ccagaatccc aacaccacca aagccttagt catatagtaa
1741 atatgtattg tgtctaggct gctgcatttc tggttatgct actgtgcagt aatacacact
1801 aatacagatg tgatggttaa tattatgtga cacttgagtg ggcacagagg tacagacact
1861 tggtaaacca ttataaggtg cacgtaagga tagttttgga tgacataaac atttagatta
1921 gtatgctggg taaaatacat tgtccatccc aatgggcatg ggctttgtca actagatgac
1981 agctggaata gaaaagctcg cctctctcat agttctcagg cctttgagct cagactagac
2041 agaactcaca ggttctctga gctttccagc ttgatgaatg tccatggcag tcttcacact
2101 taacacctga cagacttaat gatcatatga accaattcaa actctgacca tcactcgggt
2161 cagttcgttt tgattctgtc actttggaga actaataccg aggacataaa atgccatcac
2221 atcgttattt tcttcctgtc tgtgaatatt tttctttttt ttcttttttt tttttttttt
2281 tttttttttt tttttttttt ttggtttttc tctgtgtagc tttggagcct aatcctggca
2341 cttgctctgg agaccaggct gacctgaact tcagagacct cctggccttg gcctcccgag
2401 tctggggatt aaaggcgtgt accaccaacg ctcggcctgt ctgtgtaata tttaaaatga
2461 aaactttgga aatgttctga aaccagctgg tgtcagatag tcagagaact ttcgtaaggt
2521 aggtgtgggt tatagcataa tcccacacaa gagctgaagc aggaggattt tgtgtttgag
2581 ggcagctaga gcacatggtg agtccctgcc tcaaaacaca aaagcaagac aaaaacaagc
2641 tccaaataag attcacttgg gcctttcttt ccttccttct cagtgagtcc acttctttta
2701 aaatcaggtc ttaaagacgc acttagatct gaattaacag taataataaa tatcttctct
2761 tacagtacag attatgctct ataaacactg cactgataaa gttcagcctt aacctttgtt
2821 ctgtaaatgt ttcctagttt ttctactgcc gtattataag acaaatgtca gcatgaaggc
2881 aggtttttca gaaaacacag cagctcacag atgccttaat cataatcatt aaagcagctg
2941 caactttttc aactgggaaa tcattcaagg atgtttttct gaagtcccta ccagggcaca
3001 cgcacctggg ttgctgtgtg acatcagtta ggtagactct gaactggctt cccaagcaaa
3061 ttatacaaaa gcaaggtgtc acctagtatt agcataactt ctgataacta ctgtcttagc
3121 tggggtttct attgctgtga agagacacat gaccacagaa actcttataa aggaaagcaa
3181 ttattgggtc cagcttacag ttcagaggtt taatccattg tcatgattgc aggaagtatg
3241 gtggcccaca gcgagacatg gtgctggaga agtagatgag agttctatat cagattgaca
3301 cattcttcca acaaggccac actcatcact tgagctatgg gccattttca ttcaaaccac
3361 caaagctaca aggtagctta taccccagct tgctatttct gatgagactt agtaaatagt
3421 cttaaaagcc cataaaatga ctcaaaacta gtttttttat tattattatt agttcaaatt
3481 aggaagaagc ttgctttaca tgtcaatccc ttctccctct ccctcatcaa aactagtttt
3541 ttgtttttta ggtttttttt caagacaggg tttctctgtg tagctttgga gcctatccta
3601 ggcactcgct ctggagacca ggcctcgaaa tcacagtgat ctgcctgcct ttgctccgag
3661 tcctgggatc taaaggcatg caccatcaac actggccaaa ttagttttaa gtccagttct
3721 aggagctcca atgccctctt ttggcttcat gggaacagga acactatata tatatatata
3781 tatatatata tatatatata tatatatata ttcaggcaaa tatttatgca tataaaaata
3841 aaataaatct tttttccttt tttttttaaa gaagtgacat tgtcttggaa tttttgtggc
3901 tgctgtgccc ttatgtgtaa ctgacactac cagcatctaa cactggcctg aaaccagcca
3961 aagaaaaacc tttgtgccag gtcctgtgtc aaagtattat gttcctttta ggatatccta
4021 tatcctaaag gtttatttta ctgatagcat cttaacttcc tttgaaaggt tggtcttctc
4081 aagcagtcct cgtggagctg cctcctcagc taagtgccag ggacaataat tgatcccctc
4141 ccaaaaacca aaacaaaaaa ccatggcaac tctggtttcc ttggcagcac ctgctttaag
4201 aatgagcaaa tgaccaatca gctcatgaaa ctaaatactc tattattact aaaatatttt
4261 tttgagacag ggcatggaat tcatcacata gttcaggttg gccttgaact cagagagact
4321 cacttacctt tgcctcccac gtgctggaat taaaggcatg aaccaccaca ccaaacataa
4381 cacttgaatt ttgtaagagt ccttcttcca atagatttga ggttttaaaa tgtggcacag
4441 aaaatatgca attcaaatat aatcaaaaca acgacataac ttctcacact aagtttctat
4501 aggttcttgc taggaatcct aagcttgtct gaaactctag agcttctgtt tctagtcttc
4561 tgagtgttag tattgtaggt atgtgccctg cctcagtgtg atgtttttga taatcttaaa
4621 gaaatcaaag aaattttata aaagactaga ctgtgctaca caaaaagaat attcagatgc
4681 caagaaagag ttcttagaaa ttaagaaata tgctactagt ataaatcctt tataaagtgg
4741 aatgacaaat ctgatgaaat cttactaaaa gtagaaaaac ataaacatca aagacatgaa
4801 taataagaaa atcatattgt gcatatgatt aacctaaaac attaacttgc aaaaatagaa
4861 tagtccaaaa agtaaacaaa ataaataaat caccaagaac atgatacaag gacaattcct
4921 agaatgataa aacaagaata ttcattataa aaggccctat cactaaagca caacagaaac
4981 agactcaaaa gataaatctt cattgtcact ggagagaagt catactatca tagcactcag
5041 aaggaaataa aaatcaaaat gtcaaaaagg acctcagcct ctgaaacaca aatacaaaat
5101 atgtccgcct tcttgacacg cattactctt caattaacat tttaagaaaa ctataaagag
5161 agcttagtat tttaagaaat ctgtagctat ttcttttata agcatgacaa ctaagtttcc
5221 tgatttaaac agacctaaaa aaccggtgaa gtgagtggag aaaggggata cgaagacagc
5281 atcccacatg actgctccca gtaaaggcaa ggtcttcatc cattttatcc tgaactctgg
5341 gaaatttata aagaacagaa atgtatttct ctcagttctg gagcctcagt ccaggacact
5401 aagtctaggt actacactct cacatggtgg aaagtagaaa gcaagctcac ttgtcactca
5461 ctacctgatg cctctttcat caatcccatt gataaggaag agacctggca tctcagtttc
5521 ctaaggactc agctcttact aacattagct gtcatttctg ggtcactgca acagaaagcc
5581 tgacagaagc aacccagggg aagaaggatg tattttggct cactgtctct gaggatttca
5641 acttatccca gcaataaagg gataaaggca ttgcagcagg aatatgtgtg gcagaagctg
5701 tttatgtcac aataaacaaa taaacacacg ctagcgcgcg cgcacacaca cacacacaca
5761 cacacacaca cacacacaca gagagagaga gagagagaga gagagagaga gagagagaga
5821 gggggggggg cagacagaca gacagagagg gagagaggca gagagggaga gagagagaga
5881 gagagagaga gagagagaga gagagagaga gagagagaga gaaatcaaag gcccacctcc
5941 atcagactgg tcccatatcc caaatttcta gaacctccta aaacaacacc atcaactgag
6001 ggagacattt ttggattgaa agcataatgc cattacccag gcagaatctg cctgtctggg
6061 ggagtcacat ttaagccatg gtatcaattg acctcatgta atttcagaat actacataaa
6121 actatcagat atttttcatg atgaatttct aaagctt
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