GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSDHF4 1009 bp ss-mRNA ROD 27-MAY-1991
DEFINITION Mouse mRNA encoding a mutant of tetrahydrofolate dehydrogenase; 5,
6,7,8-tetrahydrofolate : NADP+ oxidoreductase, EC 1.5.1.3 which
shows low affinity for methotrexate.
ACCESSION V00734
KEYWORDS complementary DNA; dehydrogenase; oxidoreductase; reductase.
SOURCE Mus musculus RNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1009)
AUTHORS Simonsen,C.C. and Levinson,A.D.
TITLE Isolation and expression of an altered mouse dihydrofolate
reductase cDNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 2495-2499 (1983)
STANDARD full automatic
COMMENT From EMBL entry MMDHF4; dated 13-JUL-1983.
FEATURES Location/Qualifiers
mRNA 1..1009
/note="messenger RNA"
CDS 113..673
/note="DHF reductase"
/codon_start=113
BASE COUNT 292 a 220 c 237 g 260 t
ORIGIN
1 ggggcggggc cttagctgcg caagtggtac acagctcagg gctgcgattt cgcgccaaac
61 ttcacggcaa tcctagcgtg aaggctggta ggattttatc cccgctgcca tcatggttcg
121 accattgaac tgcatcgtcg ccgtgtccca aaatatgggg attggcaaga acggagaccg
181 accctggcct ccgctcagga acgagttcaa gtacttccaa agaatgacca caacctcttc
241 agtggaaggt aaacagaatc tggtgattat gggtaggaaa acctggttct ccattcctga
301 gaagaatcga cctttaaagg acagaattaa tatagttctc agtagagaac tcaaagaacc
361 accacgagga gctcattttc ttgccaaaag tttggatgat gccttaagac ttattgaaca
421 accggaattg gcaagtaaag tagacatggt ttggatagtc ggaggcagtt ctgtttacca
481 ggaagccatg aatcaaccag gccacctcag actctttgtg acaaggatca tgcaggaatt
541 tgaaagtgac acgtttttcc cagaaattga tttggggaaa tataaacttc tcccagaata
601 cccaggcgtc ctctctgagg tccaggagga aaaaggcatc aagtataagt ttgaagtcta
661 cgagaagaaa gactaacagg aagatgcttt caagttctct gctcccctcc taaagctatg
721 catttttata agaccatggg acttttgctg gctttagatc tatgagtaat tatttcttta
781 gggaggggta gttggaagaa ttgtttgttt tgtgatcttg gggatggaac ctaagaccca
841 gtgcgtgctg agcaaatgct atactgctga gccaccccaa ccctagcccc tatataattc
901 taaacaatat gttgtcattt cccagtaatc taacaaggta tagtaaaagt gccttaagaa
961 atgtcacttg ctataaaggt ctcagtgccc ctcccatgag acctcaagt
//