GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIG10A5 498 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse Ig light chain V(kappa)10-J(kappa)5 unfunctional arranged DNA ACCESSION X13935 Y00804 KEYWORDS immunoglobulin; immunoglobulin light chain; joining region; kappa-immunoglobulin; pseudogene; rearranged DNA; variable region. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 460 to 496) AUTHORS Mueller,B. and Reth,M. TITLE Ordered activation of the Ig(lambda) locus in Abelson B cell lines JOURNAL J. Exp. Med. 168, 2131-3137 (1988) STANDARD full automatic REFERENCE 2 (bases 1 to 498) AUTHORS Mueller,B. JOURNAL Unpublished (1989) STANDARD full automatic COMMENT *source: strain=NIH/Swiss; cell line=B1P8-7b Abelson 300-19 *source: derived. **map: chromosome=6. Data kindly reviewed (28-NOV-1990) by Mueller B. From EMBL entry MMIG10A5; dated 06-DEC-1990. FEATURES Location/Qualifiers misc_feature 14..62 /note="V(kappa)10 leader exon" misc_feature 188..480 /note="V(kappa)10 exon" misc_feature 481..498 /note="J(kappa) 5 exon" BASE COUNT 121 a 117 c 109 g 151 t ORIGIN 1 actcagccct gacatgatgt cctctgctca gttccttggt ctcctgttgc tctgttttca 61 aggtaaaatt gactataatg ggaattttga ctgttgcacg gtggtgattc ttgttgactg 121 gcattttgaa ggggtccttt cttttcctgc ttaactctgt gggtatttat tatgtctcca 181 ctcctaggta ccagatgtga tatccagatg acacagacta catcctccct gtctgcctct 241 ctgggagaca gagtcaccat cagttgcagg gcaagtcagg acattagcaa ttatttaaac 301 tggtatcagc agaaaccaga tggaacggtt aaactcctga tctactacac atcaagatta 361 cactcaggag tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc 421 attagcaacc tggagcaaga agatattgcc acttactttt gccaacaggg taatacgctt 481 cctcacgttc ggtgctgg //
GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIG10BR 540 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse Ig light chain V(kappa)10b-RS non-functional rearranged DNA ACCESSION X13936 Y00804 KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin; pseudogene; rearranged DNA; variable region. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 466 to 501) AUTHORS Mueller,B. and Reth,M. TITLE Ordered activation of the Ig(lambda) locus in Abelson B cell lines JOURNAL J. Exp. Med. 168, 2131-3137 (1988) STANDARD full automatic REFERENCE 2 (bases 1 to 540) AUTHORS Mueller,B. JOURNAL Unpublished (1989) STANDARD full automatic COMMENT *source: strain=NIH/Swiss; cell line=B1P8-7-3 Abelson 300-19 *source: derived. **map: chromosome=6. RS = recombining sequence Data kindly reviewed (28-NOV-1990) by Mueller B. From EMBL entry MMIG10BR; dated 06-DEC-1990. FEATURES Location/Qualifiers misc_feature 21..69 /note="V(kappa)10 leader" misc_feature 193..490 /note="V(kappa)10 exon" misc_feature 491..540 /note="RS sequence" BASE COUNT 127 a 130 c 119 g 164 t ORIGIN 1 ttccaagact cagccctgac atgatgtcct ctgctcagtt ccttggtctc ctgttgctct 61 gttttcaagg taaaattgac tataatggga attttgctgt tgcacggtgg tgattcttgt 121 tgactggcat tttgaagggg tcctttcttt tcctgcttaa ctctgtgggt atttattatg 181 tctccactcc taggtaccag atgtgatatc cagatgacac agactacatc ctccctgtct 241 gcctctctgg gagacagagt caccatcagt tgcagtgcaa gtcagggcat tagcaattat 301 ttaaactggt atcagcagaa accagatgga actgttaaac tcctgatcta ttacacatca 361 agtttacact caggagtccc atcaaggttc agtggcagtg ggtctgggac agattattct 421 ctcaccatca gcaacctgga acctgaagat attgccactt actattgtca gcagtatagt 481 aagcttcctc gaccctagtg gcagcccagg gtggatctcc ctaggactgc agttgagctc //
GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIG21E5 666 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse Ig light chain V(kappa)21E-J(kappa)5 rearranged DNA, non- functional ACCESSION X13938 Y00804 KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin; pseudogene; rearranged DNA; variable region. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 466 to 666) AUTHORS Mueller,B. and Reth,M. TITLE Ordered activation of the Ig(lambda) locus in Abelson B cell lines JOURNAL J. Exp. Med. 168, 2131-3137 (1988) STANDARD full automatic REFERENCE 2 (bases 1 to 666) AUTHORS Mueller,B. JOURNAL Unpublished (1989) STANDARD full automatic COMMENT *source: strain=NIH/Swiss; cell line=B1P8-7b Abelson 300-19 *source: derived. **map: chromosome=6. the correctly rearranged V(kappa)21E segment is not functional because it carries a 1-bp deletion in the leader sequence Data kindly reviewed (28-NOV-1990) by Mueller B. From EMBL entry MMIG21E5; dated 06-DEC-1990. FEATURES Location/Qualifiers misc_feature 60..107 /note="V(kappa)21E leader" misc_feature 341..646 /note="V(kappa)21E exon" misc_feature 647..666 /note="J(kappa)5 region" BASE COUNT 160 a 167 c 151 g 188 t ORIGIN 1 gaattcttta agggcagctg caggagccta agaagcatcc tctcttccag ctctcagaga 61 tgagacagac acactcctgt tatgggtact gctgctctgg gttccaggtg agagtgcaga 121 gaagtgttgg atgcaacctc tgtggccatt atgatactcc tagcctctct gttcttgatc 181 actataatta gggcatttgt cactggtttt aagtttcccc agtcccctga attttccatt 241 ttctcagagt gatgtccaaa attattctta aaaatttagg acctctgctg tgaaggcttt 301 tatacatata taacaataat ctttgtgttt atcattccag gttccactgg agacattgtg 361 ctgacacagt ctcctgcttc cttagctgta tctctggggc agagggccac catctcatgc 421 agggccagca aaagtgtcag tacatctggc tatagttata tgcactggta ccaacagaaa 481 ccaggacagc cacccaaact cctcatctat cttgcatcca acctagaatc tggggtccct 541 gccaggttca gtggcagtgg gtctgggaca gacttcaccc tcaacatcca tcctgtggag 601 gaggaggatg ctgcaaccta ttactgtcag cacagtaggg agcttcccac gttcggtgct 661 gggacc //
GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIG23RS 631 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse Ig light chain V(kappa)23-RS non-functional rearranged DNA ACCESSION X13937 Y00804 KEYWORDS immunoglobulin; immunoglobulin light chain; kappa-immunoglobulin; pseudogene; rearranged DNA; variable region. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 562 to 597) AUTHORS Mueller,B. and Reth,M. TITLE Ordered activation of the Ig(lambda) locus in Abelson B cell lines JOURNAL J. Exp. Med. 168, 2131-3137 (1988) STANDARD full automatic REFERENCE 2 (bases 1 to 631) AUTHORS Mueller,B. JOURNAL Unpublished (1989) STANDARD full automatic COMMENT *source: strain=NIH/Swiss; cell line=B1P8-7-2 Abelson 300-19 *source: derived. **map: chromosome=6. RS = recombining sequence Data kindly reviewed (28-NOV-1990) by Mueller B. From EMBL entry MMIG23RS; dated 06-DEC-1990. FEATURES Location/Qualifiers misc_feature 31..79 /note="V(kappa)23 leader" misc_feature 299..584 /note="V(kappa)23 exon" misc_feature 585..631 /note="RS sequence" BASE COUNT 169 a 133 c 129 g 200 t ORIGIN 1 gatccacaca aactcaggga aagctcgaag atggttttca cacctcagat tcttggactt 61 atgcttttct ggatttcagg tatgactgtt tggacatgga aataatgtgg cgatattatt 121 taaatatgaa atcttcttat tttatttgga acctagcaaa acattgtatt tgatttcctg 181 tttaaagaaa ttaatacaat gaaagtcttt tgtttttcta agtggtttag gagtgactta 241 caaattcaat gttgtgtcct actaaacttt ttttttccta attttccagc ctccagaggt 301 gatattgtgc taactcagtc tccagccacc ctgtctgtga ctccaggaga tagagtcagt 361 ctttcctgca gggccagtca aagtattagc aactacctac actggtatca acaaaaatca 421 catgagtctc caaggcttct catcaagtat gcttcccagt ccatctctgg gatcccctcc 481 aggttcagtg gcagtggatc agggacagat ttcactctca gtatcaacag tgtggagact 541 gaagattttg gaatgtattt ctgtcaacag agtaacagct ggcccctagt ggcagcccag 601 ggtggatctc cctaggactg cagttgagct c //