GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIG69 1172 bp ds-DNA ROD 27-MAY-1991
DEFINITION Mouse germ line equivalent of the rearranged V kappa gene T1 (see
entry <MMIG19>).
ACCESSION V01563
KEYWORDS germline; immunoglobulin; kappa-immunoglobulin; signal peptide;
somatic mutations; variable region.
SOURCE Mus musculus DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1172)
AUTHORS Pech,M., Hoechtl,J., Schnell,H. and Zachau,H.G.
TITLE Differences between germ-line and rearranged immunoglobulin V kappa
coding sequences suggest a localized mutation mechanism
JOURNAL Nature 291, 668-670 (1981)
STANDARD full automatic
COMMENT Sequence is from strain BALB/c. Data submitted (17-JAN-1984) on
tape to the EMBL data library by H.G. Zachau and P.S. Neumaier.
From EMBL entry MMIG69; dated 06-DEC-1983.
FEATURES Location/Qualifiers
CDS 571..619
/note="first exon (signal peptide)"
/codon_start=571
intron 620..732
/note="first intron"
CDS 733..1028
/note="second exon (signal peptide and variable region
gene L6)"
/codon_start=733
mutation 807..807
/note="point mutation, not silent"
mutation 844..844
/note="point mutation, not silent"
mutation 869..869
/note="point mutation, silent"
mutation 885..885
/note="point mutation, not silent"
mutation 955..955
/note="point mutation, not silent"
mutation 1010..1010
/note="point mutation, silent"
misc_feature 1031..1037
/note="conserved heptanucleotide box for the V-J
recombination"
BASE COUNT 333 a 234 c 232 g 373 t
ORIGIN
1 caatgtgtta tatgtgcaac tcctttgtat ttcctctctt tgaactgtag ttagcaaaag
61 cagtagaacc ttatgttttt aattcggtga aaatgtttat gataaattct ccttttaatt
121 tcaacaaatg cctactccaa acacttcact agggaggaaa tgctattatt ttagacaacc
181 aaagtaattc tattcgagtg agttaaagat ataatagttt tttctttgtt gtatattccc
241 tgacagcagg ggattcttct atattacctt agtatatctt tgaaatatat tattcaaaat
301 agagactacc tacaatagaa atgacatcta taattataaa tctttgaaaa gaaattcatt
361 ttctggagat attcaagtgg tttgacctca gtcatctggg gacactggcc ttgagttaag
421 tgctgacatg agcaagtctt gcacctgtgt ccagcaataa ctggttccca atgatttgca
481 tgctctcact tcactgcctt ggggacttct tcatataccc gtcacacatg tacggtacca
541 ttgtcattgc agccaggact cagcatggac atgaggaccc ctgctcagtt tcttggaatc
601 ttgttgctct ggtttccagg taaaatgaac taaaatggga atgtcactgt gattagtgtt
661 gattggcatt tgggagattt tatcttttat gatgcttacc tatgtagata ctcattatgt
721 ctccattcct aggtatcaaa tgtgacatca agatgaccca gtctccatct tccatgtatg
781 catctctagg agagagagtc actatcactt gcaaggcgag tcaggacatt aatagctatt
841 taagctggtt ccagcagaaa ccagggaaat ctcctaagac cctgatctat cgtgcaaaca
901 gattggtaga tggggtccca tcaaggttca gtggcagtgg atctgggcaa gattattctc
961 tcaccatcag cagcctggag tatgaagata tgggaattta ttattgtcta cagtatgatg
1021 agtttcctcc cacagtgaga caagtcataa cataaacccc catggaagca gaagtgagag
1081 gctaggctgc ctcagctgtt cctcctaatg tgtcacccac tggtattgtt tcctagatgt
1141 cacagaactt ttaagaaagc tcgtacagag tc
//