GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGF2 316 bp ds-DNA ROD 27-MAY-1991 DEFINITION Mouse non-reciprocal recombination product involving kappa V and J gene segments in the mouse myeloma MOPC173. ACCESSION V01567 KEYWORDS f-fragment; immunoglobulin; joining region; kappa-immunoglobulin; non-reciprocal recombination; rearranged DNA; variable region. SOURCE Mus musculus DNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 54) AUTHORS Sakano,H., Hueppi,K., Heinrich,G. and Tonegawa,S. TITLE Sequences at the somatic recombination sites of immunoglobulin light-chain genes JOURNAL Nature 280, 288-294 (1979) STANDARD full automatic REFERENCE 2 (bases 1 to 54) AUTHORS Max,E.E., Seidman,J.G. and Leder,P. TITLE Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene JOURNAL Proc. Natl. Acad. Sci. U.S.A. 76, 3450-3454 (1979) STANDARD full automatic REFERENCE 3 (bases 1 to 54) AUTHORS Max,E.E., Maizel,J.V.Jr.. and Leder,P. TITLE The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes JOURNAL J. Biol. Chem. 256, 5116-5120 (1981) STANDARD full automatic REFERENCE 4 (bases 1 to 316) AUTHORS Hoechtl,J., Mueller,C.R. and Zachau,H.G. TITLE Recombined flanks of the variable and joining segments of immunoglobulin genes JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79, 1383-1387 (1982) STANDARD full automatic COMMENT Sequence is taken from strain BALB/c. The sequence of the germ line DNA segment contributing its 3'-flank to this f-fragment is not yet known; probably it is a V-kappa gene region segment. Data submitted (17-JAN-1984) on tape to the EMBL data library by H.G. Zachau and P.S. Neumaier. From EMBL entry MMIGF2; dated 06-JUL-1989. FEATURES Location/Qualifiers misc_feature 1..54 /note="5'-flank of J1" misc_feature 48..54 /note="heptanucleotide box" misc_feature 55..316 /note="3'-flank of unknown germ line segment" misc_feature 55..61 /note="non-canonical heptanucleotide box" BASE COUNT 90 a 64 c 65 g 97 t ORIGIN 1 ctcttcagtg aggagggttt ttgtacagcc agacagtgga gtactaccac tgtgcacaat 61 gatataagtc ataacataaa cctccatgaa agcagaagtg tgtggtttgg cttctccaga 121 tgttcctcat ggtgaaacta tatcacagat gtcaaaggag ttttagctaa gttcatgaag 181 agttatttgt tttcatagga gggctaaaca gccctctgta tacctatctt ccttcccctt 241 catgttcagc aatacagagt accaatgtct tttctacctt aacaaaggat gcagttgctt 301 cctatgaaga gattga //