GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGF2 316 bp ds-DNA ROD 27-MAY-1991
DEFINITION Mouse non-reciprocal recombination product involving kappa V and J
gene segments in the mouse myeloma MOPC173.
ACCESSION V01567
KEYWORDS f-fragment; immunoglobulin; joining region; kappa-immunoglobulin;
non-reciprocal recombination; rearranged DNA; variable region.
SOURCE Mus musculus DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 54)
AUTHORS Sakano,H., Hueppi,K., Heinrich,G. and Tonegawa,S.
TITLE Sequences at the somatic recombination sites of immunoglobulin
light-chain genes
JOURNAL Nature 280, 288-294 (1979)
STANDARD full automatic
REFERENCE 2 (bases 1 to 54)
AUTHORS Max,E.E., Seidman,J.G. and Leder,P.
TITLE Sequences of five potential recombination sites encoded close to an
immunoglobulin kappa constant region gene
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 76, 3450-3454 (1979)
STANDARD full automatic
REFERENCE 3 (bases 1 to 54)
AUTHORS Max,E.E., Maizel,J.V.Jr.. and Leder,P.
TITLE The nucleotide sequence of a 5.5-kilobase DNA segment containing
the mouse kappa immunoglobulin J and C region genes
JOURNAL J. Biol. Chem. 256, 5116-5120 (1981)
STANDARD full automatic
REFERENCE 4 (bases 1 to 316)
AUTHORS Hoechtl,J., Mueller,C.R. and Zachau,H.G.
TITLE Recombined flanks of the variable and joining segments of
immunoglobulin genes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79, 1383-1387 (1982)
STANDARD full automatic
COMMENT Sequence is taken from strain BALB/c. The sequence of the germ line
DNA segment contributing its 3'-flank to this f-fragment is not yet
known; probably it is a V-kappa gene region segment. Data
submitted (17-JAN-1984) on tape to the EMBL data library by H.G.
Zachau and P.S. Neumaier.
From EMBL entry MMIGF2; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 1..54
/note="5'-flank of J1"
misc_feature 48..54
/note="heptanucleotide box"
misc_feature 55..316
/note="3'-flank of unknown germ line segment"
misc_feature 55..61
/note="non-canonical heptanucleotide box"
BASE COUNT 90 a 64 c 65 g 97 t
ORIGIN
1 ctcttcagtg aggagggttt ttgtacagcc agacagtgga gtactaccac tgtgcacaat
61 gatataagtc ataacataaa cctccatgaa agcagaagtg tgtggtttgg cttctccaga
121 tgttcctcat ggtgaaacta tatcacagat gtcaaaggag ttttagctaa gttcatgaag
181 agttatttgt tttcatagga gggctaaaca gccctctgta tacctatctt ccttcccctt
241 catgttcagc aatacagagt accaatgtct tttctacctt aacaaaggat gcagttgctt
301 cctatgaaga gattga
//