GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGG5BB 342 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse mRNA for IgG-1 H-chain V-D-J G5 Bb 2.2 monoclonal anti-GAT anibody (GAT = (Glu 60-Ala 30-Tyr 10)n) ACCESSION X01820 KEYWORDS diversity region; gamma-immunoglobulin; immunoglobulin; immunoglobulin heavy chain; joining region; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 342) AUTHORS Rocca-Serra,J., Matthes,H.W., Kaartinen,M., Milstein,C., Theze,J. and Fougereau,M. TITLE Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of four anti-GAT monoclonal antibodies. A paucigene system using alternate D-J recombinations to generate functionally similar hypervariable regions JOURNAL EMBO J. 2, 867-872 (1983) STANDARD full automatic COMMENT *source: strain=Balb-c; clone=G5 Bb2.2; From EMBL entry MMIGG5BB; dated 10-JAN-1991. FEATURES Location/Qualifiers misc_feature 1..275 /note="V segment (aa 7-98) (275 is 2nd base in codon)" CDS 1..342 /product="V(H) region of G5 Bb 2.2" /codon_start=1 misc_feature 276..292 /note="D segment (aa 99-103) (276 is 3rd base in codon) (292 is 1st base in codon)" misc_feature 293..342 /note="J segment (aa 104-120) (293 is 2nd base in codon)" BASE COUNT 87 a 89 c 92 g 74 t ORIGIN 1 tctggggcag agcttgtgaa gcctggggcc tcagtcaagt tgtcctgcac agcttctggc 61 ttcaacatta aagacaccta tatgcactgg gtgaagcaga ggcctgaaca gggcctggag 121 tggattggaa ggattgatcc tgcgaatggt aatactaaat atgacccgaa gttccagggc 181 aaggccacta taacagcaga cacatcctcc aacacagcct acctgcagct cagcagcctg 241 acatctgagg acactgccgt ctattactgt gctaggggat ggttacgacg tgatgctatg 301 gactactggg gtcaaggaac ctcagtcacc gtctcctcag cc //