GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGG7AB 333 bp ss-mRNA ROD 27-MAY-1991
DEFINITION Mouse mRNA for Ig gamma-1 H-chain V-D-J G7 Ab2.9 anti-GAT
monoclonal antibody (Glu 60-Ala 30-Tyr 10)
ACCESSION X07144
KEYWORDS diversity region; gamma-immunoglobulin; immunoglobulin;
immunoglobulin heavy chain; joining region; variable region.
SOURCE Mus musculus RNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 333)
AUTHORS Rocca-Serra,J., Matthes,H.W., Kaartinen,M., Milstein,C., Theze,J.
and Fougereau,M.
TITLE Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of
four anti-GAT monoclonal antibodies. A paucigene system using
alternate D-J recombinations to generate functionally similar
hypervariable regions
JOURNAL EMBO J. 2, 867-872 (1983)
STANDARD full automatic
COMMENT *source: clone=G7 Ab2.9;
From EMBL entry MMIGG7AB; dated 10-JAN-1991.
FEATURES Location/Qualifiers
misc_feature 1..266
/note="V segment (aa 7-98) (266 is 2nd base in codon)"
CDS 1..333
/product="VH region of G7 Ab 2.9"
/codon_start=1
unsure 140..140
/note="G may be A in G7 Ab 2.9"
unsure 249..249
/note="C may be A in G7 Ab 2.9"
misc_feature 267..283
/note="D segment (aa 99-103) (267 is 3rd base in codon)
(283 is 1st base in codon)"
unsure 271..271
/note="U may be A in G7 Ab 2.9"
unsure 272..272
/note="G may be U in G7 Ab 2.9"
unsure 273..273
/note="G may be A or U in G7 Ab 2.9"
misc_feature 284..333
/note="J segment (aa 104-120) (284 is 2nd base in codon)"
BASE COUNT 81 a 84 c 92 g 73 t 3 others
ORIGIN
1 gagcttgtga agcctggggc ctcagtcaag ttgtcctgca ctgcttctgg cttcaacatt
61 aaagacacct atatgcactg ggtgaaacag aggcctgaac agggcctgga gtggattgga
121 aggattgatc ctgcgaatng taatagtaaa tatggcccga agttccaggg nnaggcaact
181 ataacagcag acacatcctc cagcgcagcc tacctgcagc tcagcagcct gacatctgag
241 gacactgccg tctattactg tactaggggg tggtttcgac gggatgctgt ggactactgg
301 ggtcaaggaa cctcagtcac cgtctcctca gcc
//