GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGG8AD 320 bp ss-mRNA ROD 27-MAY-1991
DEFINITION Mouse mRNA for IgG-1 H-chain V-D-J G8 Ad 3.8 monoclonal anti-GAT
antibody (GAT = (Glu 60-Ala 30-Tyr 10)n)
ACCESSION X03219
KEYWORDS diversity region; gamma immunoglobulin; immunoglobulin;
immunoglobulin heavy chain; joining region; variable region.
SOURCE Mus musculus RNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 320)
AUTHORS Rocca-Serra,J., Matthes,H.W., Kaartinen,M., Milstein,C., Theze,J.
and Fougereau,M.
TITLE Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of
four anti-GAT monoclonal antibodies. A paucigene system using
alternate D-J recombinations to generate functionally similar
hypervariable regions
JOURNAL EMBO J. 2, 867-872 (1983)
STANDARD full automatic
COMMENT *source: clone=G8 Ad3.8; strain=Balb-c;
From EMBL entry MMIGG8AD; dated 24-APR-1990.
FEATURES Location/Qualifiers
CDS 1..320
/note="V(H) region of G8 Ad 3.8 (aa 10-116) (325 is 2nd
base in codon)"
/codon_start=1
CDS 1..267
/note="V segment (aa 10-98)"
/codon_start=1
CDS 268..285
/note="D segment (99-104)"
/codon_start=268
variation 270..270
/note="A may be C"
CDS 286..320
/note="J segment (105-116)"
/codon_start=286
BASE COUNT 90 a 80 c 84 g 66 t
ORIGIN
1 gagcttgtga agcctggggc ctcagtcaag ttgtcctgca cagcttctgg cttcaatatt
61 aaagacacct atatgcactg ggtgaagcag aggcctgaac agggcctgga gtggattgga
121 aggattgatc ctgcgaaagg taatactaaa tatgacccga agttccaggg caaggcaact
181 ataacaacag acacatcctc caacacagcc tacctgcagt tcagcagcct gacatctgag
241 gacactgcag tctactactg tgctagagga actacggtgg ggagagacta ttggggccaa
301 ggcaccactc tcacagtctc
//