GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGG8CA 336 bp ss-mRNA ROD 27-MAY-1991
DEFINITION Mouse mRNA for Ig gamma-1 H-chain V-D-J G8 Ca 1.7 anti-GAT
monoclonal antibody (Glu 60-Ala 30-Tyr 10)
ACCESSION X07143
KEYWORDS diversity region; gamma-immunoglobulin; immunoglobulin;
immunoglobulin heavy chain; joining region; variable region.
SOURCE Mus musculus RNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 336)
AUTHORS Rocca-Serra,J., Matthes,H.W., Kaartinen,M., Milstein,C., Theze,J.
and Fougereau,M.
TITLE Analysis of antibody diversity: V-D-J mRNA nucleotide sequence of
four anti-GAT monoclonal antibodies. A paucigene system using
alternate D-J recombinations to generate functionally similar
hypervariable regions
JOURNAL EMBO J. 2, 867-872 (1983)
STANDARD full automatic
COMMENT *source: clone=G8 Ca 1.7;
From EMBL entry MMIGG8CA; dated 24-APR-1990.
FEATURES Location/Qualifiers
CDS 1..336
/note="VH region of G8 Ca 1.7 (aa 7-118)"
/codon_start=1
CDS 1..275
/note="V segment (aa 7-98) (275 is 2nd base in codon)"
/codon_start=1
CDS 276..292
/note="D segment (276 is 3rd base in codon) (292 is 1st
base in codon)"
/codon_start=276
CDS 293..336
/note="J segment (aa 104-120) (293 is 2nd base in codon)"
/codon_start=293
BASE COUNT 86 a 86 c 91 g 73 t
ORIGIN
1 tctggggcag agcttgtgaa gcctggggcc tcagtcaagt tgtcctgcac agcttctggc
61 ttcaacatta aagacaccta tatgcactgg gtgaagcaga ggcctgaaca gggcctggag
121 tggattggaa ggattgatcc tgcgaatggt aatactaaat atgacccgaa gttccagggc
181 aaggccacta taacagcaga cacatcctcc aacacagcct acctgcagct cagcagcctg
241 acatctgagg acactgccgt ctattactgt gctaggggat ggttacgacg tgatgctatg
301 gactactggg gtcaaggaac ctcagtcacc gtctcc
//