GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSIGGNP5 417 bp ss-mRNA ROD 27-MAY-1991 DEFINITION Mouse mRNA for idiotypic anti-NP IgM heavy chain V-D-J (hybridoma P.9.37.1/group VI) ACCESSION X02566 KEYWORDS diversity region; immunoglobulin; immunoglobulin heavy chain; joining region; mu-immunoglobulin; signal peptide; variable region. SOURCE Mus musculus RNA. ORGANISM Mus musculus Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae. REFERENCE 1 (bases 1 to 417) AUTHORS Boersch-Supan,M.E., Agarwal,S., White-Scharf,M.E. and Imanishi-Kari,T. TITLE Heavy chain variable region Multiple gene segments encode anti-4-(hydroxy-3-nitropheny)acetyl idiotypic antibodies JOURNAL J. Exp. Med. 161, 1272-1292 (1985) STANDARD full automatic COMMENT Serological analysis of hybridomaproteins resulting from the immune response to the hapten NP reveals NP(a) idiotypes expressed by Balb/C mice and NP(b) idiotypes expressed by C57 BL/6 mice. The NP(b) family of antibodies consists of 6 subgroups (I-VI) with I-IV sharing more determinants than V and VI which appear quite distinct. From EMBL entry MMIGGNP5; dated 10-JAN-1991. FEATURES Location/Qualifiers mat_peptide join(58..147,352..373,374..417) /product="idiotypic anti-NP IgM heavy chain V-D-J" CDS join(1..147,352..373,374..417) /product="idiotypic anti-NP IgM heavy chain V-D-J" /codon_start=1 sig_peptide 1..57 misc_feature 58..147 misc_feature 58..147 /note="variable region (aa 1-98)" misc_feature 148..162 /note="CDR I" misc_feature 205..255 /note="CDR II" misc_feature 352..373 misc_feature 352..373 misc_feature 352..373 /note="D-region (aa 99-105)" misc_feature 374..>417 /note="J-region (aa 106-120)" BASE COUNT 101 a 106 c 107 g 103 t ORIGIN 1 atgaaattca gctgggtcat gttcttcctg atggcagtgg ttacaggggt caattcagag 61 gttcagctgc agcagtctgt ggcagagctt gtgaggccag gggcctcagt caagttgtcc 121 tgcacagctt ctggcttcaa cattaaaaac acctatatcc actgggtgaa gcagaggcct 181 gaacagggcc tggagtggat tggcaggatt gatcctgcga atggtaatac taaatatgcc 241 ccgaagttcc aggtcaaggc cactataact gcagacacat cctccaacac aacctacctg 301 cagctcagca gcctgacatc tgaggacact gccatctatt actgtgccct ctattactac 361 gttagtagct acgggtttgc ttactggggc caagggactc tggtcactgt ctctgca //