GenBank-Updates@genbank.bio.net (05/27/91)
LOCUS MUSTCRG04 1461 bp ss-mRNA ROD 27-MAY-1991
DEFINITION Mouse non-productive mRNA for T-cell receptor gamma V2-J2-C2
ACCESSION X04397
KEYWORDS T-cell receptor; T-cell receptor gamma; constant region;
joining region; rearranged DNA; signal peptide; variable region.
SOURCE Mus musculus RNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1461)
AUTHORS Heilig,J.S.
JOURNAL Unpublished (1986)
STANDARD full automatic
REFERENCE 2 (bases 196 to 1461)
AUTHORS Heilig,J.S. and Tonegawa,S.
TITLE Diversity of murine gamma genes and expression in fetal and adult T
lymphocytes
JOURNAL Nature 322, 836-840 (1986)
STANDARD full automatic
COMMENT *source: strain=BALB/cByJ; clone=FT12; cell-type=fetal thymocyte;
Variable/joining region junction causes frame shift resulting in
termination of presumptive translation product in J region. The
splice acceptor signal is found within presumed V2 coding region;
use of this acceptor is seen and results in truncation of V coding
region but not in frame shift.
From EMBL entry MMTCRG04; dated 02-APR-1988.
FEATURES Location/Qualifiers
misc_feature 142..1052
/note="TCR-gamma defective rearranged V-J-C coding region"
misc_feature 142..194
/note="put. leader peptide (18aa) (194 is 2nd base in
codon)"
misc_feature 195..399
/note="put. V2 segment"
misc_feature 277..289
/note="splice acceptor signal"
misc_feature 490..548
/note="J2 segment"
misc_feature 490..492
/note="premature stop codon"
CDS 549..1052
/note="C2 segment (168aa)"
/codon_start=549
BASE COUNT 432 a 353 c 281 g 395 t
ORIGIN
1 aacagatcat gaccaaccct gggggaaccc actgactgca tttttctgag actatagcac
61 agtcccaccc tccagctggt gacctgaaat tccagcctgc agagcacttc ctgcctccct
121 gtgaagcagt ccaaccttgg gatgctgctc ctgagatggt tcacctcctg ctgcctctgg
181 gtttttgggc ttgggcagct ggagcaaact gaattatcgg tcaccagaga gacagatgag
241 aatgtgcaaa tatcctgtat agtttatctt ccatatttct ccaacacagc tatacattgg
301 taccggcaaa aaacaaatca acagtttgag tatctaatat atgtcgcaac aaactacaat
361 caacgaccct taggagggaa gcacaaaaaa attgaagcaa gtaaagattt taaaagttct
421 acctcaacct tggaaataaa ttacttgaag aaagaagatg aagccaccta ctactgtgca
481 gtctggatat agctcgggct ttcacaaggt atttgcagaa ggaacaaagc tcatagtaat
541 tccctccgac aaaaggcttg atgcagacat ttcccccaag cccactattt ttcttccttc
601 tgttgctgaa acaaatctcc ataagactgg gacatacctt tgtctccttg aaaagttctt
661 tcccgatgtc ataagggtgt attggaaaga aaagaatggc aatactatcc tggactccca
721 ggaaggggat acgctgaaga ctaagggcac atacatgaag tttagctggc ttactgtgcc
781 tgaaagggca atggggaaag agcacagttg tattgtcaaa catgagaaca acaaaggagg
841 agcagatcaa gagattttct tcccttcaat aaagaaagtt gctacaactt gctggcaaga
901 taaaaatgat gtgctgcagt tccagttcac gagcacctct gcctactaca cctacctcct
961 cctgctcctc aagagtgtga tctacttggc catcatcagc ttctctctgc ttagaagaac
1021 atctgtctgt ggcaatgaga agaagtccta aagaaacgag tggtggtaca gcaagtcagc
1081 tggatttcat cctcactgcc ataaaggtgc cttaaggggg aaccagatgc cttctcttgt
1141 tggctttcac ttctataaag tccctcactc atgttgcata aacattttct gaacttttgt
1201 atgcaatttc agcaactttt tttaaactga actcaccttc ttcctgattc catccactcc
1261 agaagtcccc tcccccagaa gcctgaaaca ttaaaattct agtacccata gcctacagct
1321 tttacccatg gcccttgttg ctcttgaacc agctaactcc atgaagaccc ccactctaga
1381 caacctgaag agccttcttt actgtttgat tctgtgcctt gtatttcaga ttattgacaa
1441 taaatttttt aaaaaattaa a
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