GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS HIVVD194 9472 bp ds-DNA VRL 28-MAY-1991
DEFINITION Human immunodeficiency virus type 2 strain D194 proviral genome
ACCESSION X52223
KEYWORDS RNase H; env gene; envelope glycoprotein; gag gene; integrase;
long terminal repeat; nef gene; pol gene; polymerase;
reverse transcriptase; tat gene; vif gene; vpr gene; vpx gene.
SOURCE Human immunodeficiency virus type 2 DNA.
ORGANISM Human immunodeficiency virus type 2
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Retroviridae; Lentivirinae.
REFERENCE 1 (bases 1 to 9472)
AUTHORS Kuehnel,H.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Kuehnel,H., Kreutz,R. and Ruebsamen-Waigmann,H.
TITLE Nucleotide sequence of HIV-2 (D194), an isolate from a Gambian case
of 'Neuro-AIDS', which showed excellent growth in macrophages
JOURNAL Nucleic Acids Res. 18, 6142-6142 (1990)
STANDARD full staff_review
COMMENT *source: strain=D194; cell type=cord blood lymphocyte; Virus DNA
from a genomic library of infected cord blood lymphocytes. HIV-2
strain D194 was isolated in 1987 from peripheral blood of a Gambian
male with neurological manifestations of AIDS. Ribosomal frame
shift gives rise to pol gene product. See also <J04542> for
overlapping sequences.
From EMBL entry HIVD194; dated 07-JAN-1991.
FEATURES Location/Qualifiers
misc_feature 1..173
/note="R region"
misc_feature 174..299
/note="U5 region"
CDS 547..2109
/note="gag gene product (AA 1-521)"
/codon_start=547
CDS 1839..4934
/note="starting with the site of ribosomal frameshift"
/codon_start=1839
CDS 4867..5511
/note="vif gene product (AA 1-215)"
/codon_start=4867
CDS 5342..5677
/note="vpx gene product (AA 1-112)"
/codon_start=5342
CDS 5680..5994
/note="vpr gene product (AA 1-105)"
/codon_start=5680
CDS 5843..6138
/note="tat gene product exon 1 (AA 1-99) (6138 is 2nd base
in codon)"
/codon_start=5843
CDS 6069..6138
/note="rev gene product exon 1 (AA 1-23) (6138 is 1st base
in codon)"
/codon_start=6069
intron 6139..8274
/note="intron"
CDS 6145..8697
/note="env gene product (AA 1-851)"
/codon_start=6145
CDS 8275..8513
/note="rev gene product exon 2 (AA 24-103) (8275 is 2nd
base in codon)"
/codon_start=8275
CDS 8275..8368
/note="tat gene product exon 2 (AA 100-130) (8275 is 3rd
base in codon)"
/codon_start=8275
CDS 8534..9304
/note="nef gene product (AA 1-257)"
/codon_start=8534
misc_feature 8915..9471
/note="U3 region"
BASE COUNT 3220 a 1946 c 2382 g 1924 t
ORIGIN
1 agtcgctctg cggagaggct ggcagattga gccctgggag gttctctcca gcactagcag
61 gtagagcctg ggtgttccct gctagactct caccagtgct tggccggcac tgggcagacg
121 gctccacgct tgcttgctta aagacctctt aataaagctg ccagttagaa gcaagttaag
181 tgtgtgttcc catctctcct agtcgccgcc tggtcattcg gtgttcatct gagtaacaag
241 accctggtct gttaggaccc ttcccgcttt gagaatccaa ggcaggaaaa tccctagcag
301 gttggcgccc gaacagggac ttgaaagagg actgagaagc cctggaacac ggctgagtga
361 aggcagtaag ggcggcagga acaaaccacg acggagtgct cctagaaaag cgcgggccga
421 ggtaccgaag cggcgtgtgg agcgggagtg aaagaggcct ccgggtgaag gtaagtacct
481 acaccgaaaa ctgtagccag aaaaggcttg ttatcctacc tttagacagg tagaagattg
541 tgggagatgg gcgcgagaaa ctccgtcttg agagggaaaa aagcagacga attagaaaaa
601 gttaggttac ggcccaacgg aaagaaaaga tacaggttaa aacatgttgt gtgggcagcg
661 aatgaattag acagattcgg attggcagag agcctgttgg aatcaaaaga aggttgccaa
721 aagattctta aagttttaga accattagta ccaacagggt cagaaaattt aaaaagcctt
781 tttaataccg tctgcgtcat ttggtgcttg cacgcagaag agaaagtgaa agatactgaa
841 gaagcaaaga aactagcaca gagacatcta gtggcagaaa caggaactgc agagaaaatg
901 ccaaatataa gtagaccaac agcaccacct agtgggaaag gaggaaactt ccccgtgcaa
961 caggcaggcg gcaactatat ccatgtgccg ctgagccccc gaactctaaa tgcttgggta
1021 aaattagtag aggaaaagaa gttcggggca gaagtagtgc caggatttca ggcactctca
1081 gaaggctgca cgccctatga tatcaatcaa atgcttaatt gtgtgggcga tcaccaagca
1141 gctatgcaaa taatcagaga aattattaat gaggaagcag cagattggga tgcgcagcac
1201 ccaataccag gccccttacc agcagggcag cttagagacc caagggggtc tgacatagca
1261 ggaacaacaa gcacagtaga tgaacagatc cagtggatgt ataggcaacc aaatcccgtg
1321 ccggtaggga acatctacag gagatggatc cagatagggc tacagaaatg tgtcaggatg
1381 tacaacccaa ctaacatctt agatgtgaag cagggaccaa aagaatcgtt ccagagctat
1441 gtagacagat tctacaaaag cctaagggca gaacaaacag acccggctgt aaaaaattgg
1501 atgacccaaa cgctgctaat acagaatgcc aacccagact gcaagttagt attaaaagga
1561 ctagggatga atcccaccct agaggagatg ctgactgcct gccagggagt aggcggacca
1621 agccagaaag ccagactaat ggctgaagcc ctaaaggagg ctttgacgcc agcccctatc
1681 ccatttgcag cagcccaaca aagaagggca attaggtgtt ggaattgtgg aaaggaggga
1741 cactcggcga aacagtgccg agcacccaga agacagggct gctggaagtg tggcaagtca
1801 ggacacatca tggcaaactg cccggaaaga caggcaggtt ttttagggat gggcccacgg
1861 ggaaagcagc cccgcaactt ccccgcggcc caagctcctc aggggctgat accaacagca
1921 cccccaatag atccagcagt ggacctgttg gagaaatata tgcagcaagg gagaaagcag
1981 agagagcaga gggagagacc atacaaggag gtgacggagg acttactgca cctcgagcag
2041 ggagagacgc cccacagagg ggcgacagag gacttgctac acctcaattc tctctttgga
2101 aaagaccagt agtcacagca ttcatcgagg atcagccggt agaagtctta ctagacacag
2161 gagctgatga ctcaatagta gcaggaatag agttagggga caattacact ccaaaaatag
2221 tggggggaat agggggattc ataaatacca aagaatataa aaatgtagaa ataaaggtac
2281 taaataaaag agtaagagcc accataatga caggagatac cccaatcaac atttttggca
2341 gaaatattct ggcaacctta ggcatgtcat taaacctacc agtcgccaag ttagacccaa
2401 taaaagtaac attgaagcca gggaaagatg gaccaaggct gaaacaatgg cccctaacaa
2461 aagaaaaaat agaagcacta aaagaaattt gtgaaaaaat ggaaagggag ggccaactag
2521 aagaagcacc tccaactaat ccttataata cccccacatt tgcaattaag aaaaaggaca
2581 agaacaaatg gagaatgcta atagatttta gagaactaaa cagggtgact caagatttca
2641 cagaaattca gctaggaatt ccacacccgg caggattagc caaaaagaaa aggattactg
2701 tactagatgt aggggatgcc tacttttcca taccactaca tgaagatttt aggcaatata
2761 ctgcatttac cctaccatca gtaaacaatg cagagccaga aaaaagatat gtatataagg
2821 tcttaccaca aggatggaaa ggatcaccag caatctttca attcatgatg aggcaaatct
2881 tagaaccttt cagaaaagca aacccagacg tcattctcat ccaatacatg gatgatatct
2941 taatagctag tgacaggacg ggtttagagc atgacaaagt agtcctgcaa ctaaaagaac
3001 ttctgaatgg cctagggttc tctaccccag atgagaagtt ccaaaaggac cctccgtttc
3061 aatggatggg ctatgaattg tggccaacta aatggaaact gcagaaaata caattacctc
3121 agaaagaaat atggacagtc aatgacatcc aaaaactagt aggagttttg aactgggcgg
3181 cgcagatcta tccagggata aaaaccaagc atttatgtaa attgattaga ggaaaaatga
3241 cactcacaga ggaagtacag tggacagagt tagcagaggc agaactagaa gaaaacaaaa
3301 ttatcttaag tcaggaacaa gagggatcct actatcagga agaagaagaa ctagaagcaa
3361 cagtcatcaa aagccaagac aatcagtggg catacaaaat acaccaggga gagagggttc
3421 taaaagtagg aaagtatgcg aagataaaaa atactcatac caatggggtc agactactag
3481 cacaagtagt ccaaaaaata ggaaaggaag cactggtcat ttggggacga gtgccaaaat
3541 ttcacctacc ggtagagaga gacacctggg agcaatggtg ggataactac tggcaagtaa
3601 catgggtccc agagtgggac ttcgtatcta ccccaccact ggtcaggttg acatttaact
3661 tggtaggaga tcctatacca ggcacagaga ccttttacac agatggatca tgcaatagac
3721 agtcaaaaga aggaaaagca ggatatgtaa cagatagagg gagagacagg gtaagagtat
3781 tagagcaaac atccaatcag caagcagaac tagaagcctt tgcgatggca ctggcagact
3841 caggtcccaa ggttaatatc atagtagact cacagtatgt aatggggata gtagcaggcc
3901 aaccaacaga gtcagaaaat agaatagtaa accaaatcat tgaggacatg ataaagaaag
3961 aagcagtcta tgttgcatgg gtcccagccc ataaaggcat aggaggaaac caggaagtag
4021 accatttagt aagtcagggc atcagacaag tattattcct ggaaaagata gagcccgctc
4081 aagaagaaca cgaaaaatat catagcaata taaaagaact aacccataaa tttggaatac
4141 cccaactagt ggcaagacag atagtaaaca catgtgccca atgccaacag aaaggagaag
4201 ccatacatgg gcaagtaaat gcagaaatag gcgtttggca aatggactgc acacacttag
4261 aaggaaaaat cattatagta gcagtgcatg ttgcaagtgg attcatagaa gcagaagtca
4321 tcccacagga atcaggaagg cagacagcac tcttcctatt aaaactggcc agtaggtggc
4381 caataacaca cttgcacaca gacaatggcc ccaacttcac ttcacaggaa gtgaagatgg
4441 tggcatggtg gataggtata gagcaatcct ttggagtacc ttacaatcca caaagccagg
4501 gagtagtaga agcaatgaat caccacctaa aaaatcagat aagtagaatt agagaacagg
4561 caaatacaat agaaacaata gtactaatgg cagttcattg catgaatttt aaaagaaggg
4621 gaggaatagg ggatatgacc ccagcagaaa gactaattaa catgatcacc acagaacaag
4681 aaatacaatt cctccaaaga aaaaattcaa attttaaaaa attccaggtc tattacagag
4741 aaggcagaga tcagctgtgg aaaggacctg gagagctact gtggaaggga gacggagcag
4801 tcatagtcaa ggtaggggcg gacataaaag tagtaccaag aaggaaggcc aagattatca
4861 gggactatgg aggaaggcaa gaactggata gtagttccca cctggagggt gccagggagg
4921 atggagaggt ggcatagcct tgtcaagcac ctgaagtaca gaacaaaaga cttagaggag
4981 gtgcgctatg ttccccatca caaggtagga tgggcatggt ggacttgcag cagggtaata
5041 ttcccactag aaggagaaag tcatctagag atacaggcat attggaacct aacaccagaa
5101 aaaggatggc tctcctctca ttcagtaagg ttaacctggt atacagaaaa gttctggaca
5161 gatgttaccc cagactgtgc agactcccta atacacagca cttatttctc ttgctttacg
5221 gcaggtgaag taagaagagc catcagaggg gaaaagttat tgtcctgctg caactacccc
5281 caagctcata aagcacaggt accatcactt caatacctag ccctagtggt agtgcaacaa
5341 aatggcagac cccagagaaa gggtgccgcc aggaaacagt ggagaagaga ccattggaga
5401 ggccttcgag tggctagaca ggactataga agccttaaac agggaggcag tgaaccatct
5461 gccccgagag ctcattttcc aggtgtggca aaggtcctgg gcatattggc atgatgaaca
5521 agggatgtca acaagttaca caaagtatag atatttgtgc ataatgcaga aagctgtgta
5581 tatacatttc aagaaggggt gcacttgcct ggggagagga catggcccgg gaggatggag
5641 accaggacct ccccctcctc cccctccagg tctagtctaa tgactgaagc accaacagag
5701 tttcccccag aagatgggac cccacggaga gagctaggga gtacctgggt aatagaaact
5761 ctgaaggaaa tcaaggaaga agccttaaaa cattttgatc cctgcttgct aattgctctt
5821 ggcaactata tctataatag acatggagac acccttgaag gagccagaga gctcattaga
5881 gtcctacaac gagccctctt cgtgcacatc agagcgggat gtgaccgctc aagaaagggc
5941 caaacaagga gaagagctcc ttgcccagct gcaccgaccc ctagaggcat gcactaactc
6001 atgctattgt aagcagtgca gttaccattg ccagctgtgt ttcttgaaaa aagggctcgg
6061 gatatggtat gcgcgacagg gcagacgaag aaggactcca agaaaaacta agactcatcc
6121 gcctcctgca tcagataagt aagtatggag cctggtagga atcagctgct tgttgccatt
6181 ttattaacta gtgcttgctt aatatattgc aaacaatatg tgactgtttt ctatggcata
6241 cccgcgtgga gaaatgcatc tattccccta ttttgtgcaa ccaaaaatag agatacttgg
6301 gggaccatcc agtgcttgcc agacaatgat gattatcagg aaataacctt aaatgtgaca
6361 gaagcttttg atgcatggga taatacagta acagaacaag caatagaaga tgtctggaga
6421 ctgtttgaga catcaataaa accatgtgtc aagttgacgc ccctatgtgt ggcgatgaat
6481 tgtaatataa cttcagggac taccgcgacc ccgagtccac caaacattac aataatagat
6541 gaaaattcta cctgtatagg cgacaacaac tgcacaggat tagggaaaga agaggtggtt
6601 gagtgtgagt tcaatatgac ggggctagaa caagataaga aaaggaagta taatgacgca
6661 tggtactcaa gagatgtggt ttgtgacaag acaaacggaa caggcacatg ttacatgaga
6721 cattgcaaca catcagtcat caaagagtca tgtgacaagc actattggga tgctatgaag
6781 tttagatact gtgcaccacc gggttttgcc ctactaagat gcaatgatac caactattca
6841 ggctttgaac ctaagtgctc taaagtagta gctgcttcat gcacaaggat gatggaaacg
6901 caaacttcta cttggtttgg ctttaatggc actagagcag aaaatagaac atatatctat
6961 tggcatggta aggataatag gactatcatt agcttaaaca agtattataa tctcacaatg
7021 cattgtaaga gaccaggaaa taagacagtt gtaccaataa cacttatgtc agggcgaagg
7081 tttcactctc ggccagtcta caacaaaaaa cctgggcagg catggtgttg gtttcaaggc
7141 aactggatag aagccatgcg ggaggtgaag caaacccttg caaaacatcc caggtacgga
7201 ggaacaaatg atacaggaaa aattaacttt acgaagccag gaataggttc agacccagaa
7261 gtgacataca tgtggactaa ctgcagagga gaatttctct actgtaatat gacttggttc
7321 ctcaattggg tagaaaataa gacgaaccaa acacacggca actatgcgcc atgccatata
7381 aggcagataa ttaacacctg gcataaggta gggacaaatg tatatttgcc tcctagggaa
7441 ggggagttga cctgcaattc aacagtaacc agcataattg ctaacattga ctcagatgga
7501 aatcagacca acattacctt tagtgcagaa gtggcagaac tgtaccgatt agaattgggg
7561 gactacaaat tgatagaagt aacaccaatt ccgttcgcac ctacaaaaga gaaaagatat
7621 tcctcggctc cagtgaggaa caaaagaggt gtgttcgtgc tagggttctt gggttttctc
7681 gcgacagcag gttctgcaat gggcggcgcg tccttgacgc tgtcggctca gtcccggact
7741 ttactggccg ggatagtgca gcaacagcaa cagctgttgg acgtggtcaa gagacaacaa
7801 gaaatgttgc gattgaccgt ctggggaacg aaaaatctcc aggcaagagt cactgctatc
7861 gagaaatact taaaggacca ggcacagcta aattcatggg gatgtgcgtt taggcaggtc
7921 tgccacacta ctgtaccatg ggtaaatgac tccttaacac ctgactggaa caatatgaca
7981 tggcaggaat gggaaaaacg agtccactac ctagaggcaa atatcagtca aagtttagaa
8041 caggcacaaa ttcaacaaga aaagaatatg tatgaactac aaaaactaaa tagctgggat
8101 gtctttggca actggtttga tttgacctcc tggatcaaat atattcaata tggagtttat
8161 atagtagtag gaataatagg tttaagaata gccatatata tagtgcaatt gttaagtaga
8221 cttagaaagg gctataggcc tgttttctcc tccccccccg gttatctcca acagatccat
8281 atccacacgg acaggggaca gccagccaac gaagaaacag aagaagacgc cggagacgac
8341 agtggtttcg gcttgtggcc ttggccacta aactacatac aattcctgat ccacctactg
8401 actcgcctct tgaccgggct atacaacagc tgcaggggct tactatccaa gaactccccg
8461 acccgccgac tgatctccca gagtctaaca gcaatcaggg actggctgag acttaaggcg
8521 gcctacctgc aatatgggtg cgagtggatc caagaagcgt tccgagcatt cgcaaggact
8581 gcgagagaga ctattgcggg cgcgtggagg gggttatgtg aagcagcgca acgcatcggg
8641 aggggaatcc tcgcagtccc aagaaggatc aggcagggag cagaaatcgc cctcctgtga
8701 gggacagcag tatcagcagg gagagttcat gaacacccca tggagaaccc cagcagcaat
8761 agggcagaaa aattcatata agcagcaaaa tatggatgat gtagattctg atgatgatga
8821 cctagtggga gttcctgtta tgccaagagt accgctgaga gaaatgacct ataaactggc
8881 aatagatatg tcacatttta taaaagaaaa aggaggactg gaagggatat tttacagtag
8941 ggagagacat agaatcctag acttgttcct agaaaaggag gaagggataa taccagattg
9001 gcagaattat actcatgggc caggaacaag gtacccaatg tacttcgggt ggctgtggaa
9061 actagtacca gtagacatct cacaagaggc agaggaagta gagaccaact gcttagtaca
9121 cccagcacaa acaagcagat atgatgacga gcatggggag acactagttt ggcggtttga
9181 ccccatgctg gcctatagtt acaaggcctt cattctgcac ccagaagaat ttgggcacaa
9241 gtcaggattg ccagagaaag agtggaaggc aaaactgaaa gcaagaggga taccatatag
9301 tgaataacag gaacaaccat acttggtcag ggcaggaaat agctactaag aacagctgag
9361 actgcaggga ctttccagaa ggggctgtaa ccaagggagg gacatgggag gagctggtgg
9421 ggaacgccct catattctct gtataaatgt acccgcttct tgcattgtat tc
//