GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS POLLIO1A 7433 bp ss-mRNA VRL 28-MAY-1991
DEFINITION Genome of human poliovirus type 1 (Mahoney strain). (One of two
versions.)
ACCESSION V01148
KEYWORDS coat protein; genome; polyprotein.
SOURCE Poliovirus type 1 RNA.
ORGANISM Poliovirus type 1
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Picornaviridae; Enterovirus.
REFERENCE 1 (bases 5355 to 5522)
AUTHORS Kitamura,N., Adler,C.J., Rothberg,P.G., Martinko,J., Nathenson,S.G.
and Wimmer,E.
TITLE The Genome-Linked Protein of Picornaviruses. VII. Genetic Mapping
of Poliovirus VPg by Protein and RNA Sequence Studies
JOURNAL Cell 21, 295-302 (1980)
STANDARD full automatic
REFERENCE 2 (bases 6375 to 7433)
AUTHORS Kitamura,N. and Wimmer,E.
TITLE Sequence of 1060 3'-Terminal nucleotides of poliovirus RNA as
determined by a modification of the dideoxynucleotide method
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 3196-3200 (1980)
STANDARD full automatic
REFERENCE 3 (bases 1 to 7433)
AUTHORS Kitamura,N., Semler,B.L., Rothberg,P.G., Larsen,G.R., Adler,C.J.,
Dorner,A.J., Emini,E.A., Hanecak,R., Lee,J.J., Van Der Werf,S.,
Anderson,C.W. and Wimmer,E.
TITLE Primary structure, gene organization and polypeptide expression of
poliovirus RNA
JOURNAL Nature 291, 547-553 (1981)
STANDARD full automatic
REFERENCE 4 (bases 1 to 7432)
AUTHORS Racaniello,V.R. and Baltimore,D.
TITLE Molecular cloning of poliovirus cDNA and determination of the
complete nucleotide sequence of the viral genome
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 4887-4891 (1981)
STANDARD full staff_review
REFERENCE 5 (sites)
AUTHORS Emini,E.A., Elzinga,M. and Wimmer,E.
TITLE Carboxy-Terminal Analysis of Poliovirus Proteins: Termination of
Poliovirus RNA Translation and Location of Unique Poliovirus
Polyprotein Cleavage Sites
JOURNAL J. Virol. 42, 194-199 (1982)
STANDARD full staff_review
REFERENCE 6 (sites)
AUTHORS Dorner,A.J., Dorner,L.F., Larsen,G.R., Wimmer,E. and Anderson,C.W.
TITLE Identification of the Initiation Site of Poliovirus Polyprotein
Synthesis
JOURNAL J. Virol. 42, 1017-1028 (1982)
STANDARD full staff_review
COMMENT SWISS-PROT; P03299; POLG$POL1M.
This entry was previously called PIPVX1Z1. It replaces that entry
and the entries PIPVX1 and PIPVX2.
See also entries <POLIO1B> (for another version of this genome) and
<POLIOS1> (for the genome of the Sabin 1 strain).
The sequence and features listed here are taken from [3]; conflicts
with the other references are noted in the feature table. Note
that [3] reports only one strand sequenced. The sequence reported
in [4] is available in entry <POLIO1B>. Post-translational
proteolytic cleavage of polyprotein NCVPOO produces the other
peptides indicated below. Identification of reading frames is
preliminary.
From EMBL entry POLIO1A; dated 06-JUL-1989.
FEATURES Location/Qualifiers
conflict 284..286
/note="GUU is CGCTC in [4]"
/citation=[4]
conflict 350..350
/note="U is G in [4]"
/citation=[4]
conflict 385..385
/note="C is GC in [4]"
/citation=[4]
conflict 485..486
/note="GU is G in [4]"
/citation=[4]
CDS 698..7364
/note="open reading frame"
/codon_start=698
CDS 741..7361
/note="reading frame NCVPOO"
/codon_start=741
CDS 744..3380
/note="reading frame P1-1a"
/codon_start=744
CDS 744..1760
/note="reading frame P1-VPO"
/codon_start=744
CDS 744..947
/note="reading frame P1-VP4"
/codon_start=744
CDS 948..1760
/note="reading frame P1-VP2"
/codon_start=948
conflict 1465..1465
/note="G is GG in [4]"
/citation=[4]
conflict 1526..1526
/note="C is CC in [4]"
/citation=[4]
conflict 1530..1530
/note="C is CC in [4]"
/citation=[4]
conflict 1542..1542
/note="U is C in [4]"
/citation=[4]
conflict 1574..1574
/note="A is C in [4]"
/citation=[4]
conflict 1596..1596
/note="C is A in [4]"
/citation=[4]
conflict 1663..1663
/note="U is C in [4]"
/citation=[4]
CDS 1761..2474
/note="reading frame P1-VP3"
/codon_start=1761
conflict 1996..1996
/note="C is A in [4]"
/citation=[4]
conflict 1999..2002
/note="CCU is AUC in [4]"
/citation=[4]
conflict 2030..2030
/note="C is U in [4]"
/citation=[4]
conflict 2128..2128
/note="C is U in [4]"
/citation=[4]
conflict 2281..2281
/note="G is C in [4]"
/citation=[4]
CDS 2475..3380
/note="reading frame P1-VP1"
/codon_start=2475
conflict 2978..2978
/note="A is G in [4]"
/citation=[4]
conflict 3038..3038
/note="G is A in [4]"
/citation=[4]
conflict 3298..3298
/note="G is GG in [4]"
/citation=[4]
conflict 3302..3303
/note="GC is G in [4]"
/citation=[4]
CDS 3381..5105
/note="reading frame P2-3b"
/codon_start=3381
conflict 3652..3652
/note="U is C in [4]"
/citation=[4]
conflict 3691..3691
/note="A is C in [4]"
/citation=[4]
conflict 3761..3761
/note="A is C in [4]"
/citation=[4]
CDS 3828..5105
/note="reading frame P2-5b"
/codon_start=3828
CDS 4119..5105
/note="reading frame P2-X"
/codon_start=4119
conflict 4154..4155
/note="CC is C in [4]"
/citation=[4]
conflict 4159..4159
/note="G is GC in [4]"
/citation=[4]
conflict 4169..4169
/note="C is A in [4]"
/citation=[4]
CDS 5106..7361
/note="reading frame P3-1b"
/codon_start=5106
conflict 5108..5108
/note="C is A in [4]"
/citation=[4]
CDS 5367..5432
/note="reading frame P3-VPg"
/codon_start=5367
CDS 5433..7361
/note="reading frame P3-2"
/codon_start=5433
CDS 5433..5978
/note="reading frame P3-7c"
/codon_start=5433
conflict 5593..5593
/note="C is U in [4]"
/citation=[4]
conflict 5614..5618
/note="UGUUU is CGCUC in [4]"
/citation=[4]
conflict 5640..5640
/note="U is C in [4]"
/citation=[4]
conflict 5781..5781
/note="C is G in [4]"
/citation=[4]
conflict 5898..5899
/note="AA is AAA in [4]"
/citation=[4]
conflict 5921..5923
/note="GGA is GGGAA in [4]"
/citation=[4]
conflict 5962..5963
/note="UA is AC in [4]"
/citation=[4]
CDS 5979..7361
/note="reading frame P3-4b"
/codon_start=5979
conflict 5989..5989
/note="C is A in [4]"
/citation=[4]
conflict 6011..6011
/note="C is A in [4]"
/citation=[4]
conflict 6013..6013
/note="C is U in [4]"
/citation=[4]
conflict 6253..6253
/note="U is C in [4]"
/citation=[4]
conflict 6837..6839
/note="CCA is CA in [2]"
/citation=[1]
conflict 6970..6971
/note="UU is UUU in [2]"
/citation=[1]
conflict 7250..7250
/note="U is UU in [2]"
/citation=[1]
conflict 7402..7402
/note="C is U in [4]"
/citation=[4]
conflict 7432..7433
/note="GG is G in [4]"
/citation=[4]
BASE COUNT 2200 a 1732 c 1708 g 1793 t
ORIGIN
1 ttaaaacagc tctggggttg tacccacccc agaggcccac gtggcggcta gtactccggt
61 attgcggtac ccttgtacgc ctgttttata ctcccttccc gtaacttaga cgcacaaaac
121 caagttcaat agaagggggt acaaaccagt accaccacga acaagcactt ctgtttcccc
181 ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg gatccgttat ccgcttatgt
241 acttcgagaa gcccagtacc acctcggaat cttcgatgcg ttggttagca ctcaacccca
301 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgt tccaggctgc
361 gttggcggcc tacctatggc taacgcatgg gacgctagtt gtgaacaagg tgtgaagagc
421 ctattgagct acataagaat cctccggccc ctgaatgcgg ctaatcccaa cctcggagca
481 ggtggttcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
541 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
601 ttgttatcat aaagcgaatt ggattggcca tccggtgaaa gtgagactca ttatctatct
661 gtttgctgga tccgctccat tgagtgtgtt tactctaagt acaatttcaa cagttatttc
721 aatcagacaa ttgtatcata atgggtgctc aggtttcatc acagaaagtg ggcgcacatg
781 aaaactcaaa tagagcgtat ggtggttcta ccattaatta caccaccatt aattattata
841 gagattcagc tagtaacgcg gcttcgaaac aggacttctc tcaagaccct tccaagttca
901 ccgagcccat caaggatgtc ctgataaaaa cagccccaat gctaaactcg ccaaacatag
961 aggcttgcgg gtatagcgat agagtactgc aattaacact gggaaactcc actataacca
1021 cacaggaggc ggctaattca gtagtcgctt atgggcgttg gcctgaatat ctgagggaca
1081 gcgaagccaa tccagtggac cagccgacag aaccagacgt cgctgcatgc aggttttata
1141 cgctagacac cgtgtcttgg acgaaagagt cgcgagggtg gtggtggaag ttgcctgatg
1201 cactgaggga catgggactc tttgggcaaa atatgtacta ccactaccta ggtaggtccg
1261 ggtacaccgt gcatgtacag tgtaacgcct ccaaattcca ccagggggca ctaggggtat
1321 tcgccgtacc agagatgtgt ctggccgggg atagcaacac cactaccatg cacaccagct
1381 atcaaaatgc caatcctggc gagaaaggag gcactttcac gggtacgttc actcctgaca
1441 acaaccagac atcacctgcc cgcagttctg cccggtggat tacctccttg gaaatggcac
1501 gttgttgggg aatgcctttg tgttccgcac agataataaa cttacggacc aacaactgtg
1561 ctacactggt actaccttac gtgaactccc tctcgctaga tagtatggta aagcacaata
1621 attggggaat tgcaatatta ccattggccc cattaaattt tgttagtgag tcctccccag
1681 agattccaat caccttgacc atagccccta tgtgctgtga gttcaatgga ttaagaaaca
1741 tcaccctgcc acgcttacag ggcctgccgg tcatgaacac ccctggtagc aatcaatatc
1801 ttactgcaga caacttccag tcaccgtgtg cgctgcctga atttgatgtg accccaccta
1861 ttgacatacc cggtgaagta aagaacatga tggaattggc agaaatcgac accatgattc
1921 cctttgactt aagtgccaca aaaaagaaca ccatggaaat gtatagggtt cggttaagtg
1981 acaaaccaca tacagccgcc tccatactct gcctgtcact ctctccagcc tcagatccta
2041 ggttgtcaca tactatgctt ggagaaatcc taaattacta cacacactgg gcaggatccc
2101 tgaagttcac gtttctgttc tgtggatcca tgatggcaac tggcaaactg ttggtgtcat
2161 acgcgcctcc tggagccgac ccaccaaaga agcgtaagga ggcgatgttg ggaacacatg
2221 tgatctggga cataggactg cagtcctcat gtactatggt agtgccatgg attagcaaca
2281 gcacgtatcg gcaaaccata gatgatagtt tcaccgaagg cggatacatc agcgtcttct
2341 accaaactag aatagtcgtc cctctttcga cacccagaga gatggacatc cttggttttg
2401 tgtcagcgtg taatgacttc agcgtgcgct tgttgcgaga taccacacat atagagcaaa
2461 aagcgctagc acaggggtta ggtcagatgc ttgaaagcat gattgacaac acagtccgtg
2521 aaacggtggg ggcggcaaca tctagagacg ctctcccaaa cactgaagcc agtggaccaa
2581 cacactccaa ggaaattccg gcactcaccg cagtggaaac tggggccaca aatccactag
2641 tcccttctga tacagtgcaa accagacatg ttgtacaaca taggtcaagg tcagagtcta
2701 gcatagagtc tttcttcgcg cggggtgcat gcgtgaccat tatgaccgtg gataacccag
2761 cttccaccac gaataaggat aagctatttg cagtgtggaa gatcacttat aaagatactg
2821 tccagttacg gaggaaattg gagttcttca cctattctag atttgatatg gaacttacct
2881 ttgtggttac tgcaaatttc actgagacta acaatgggca tgccttaaat caagtgtacc
2941 aaattatgta cgtaccacca ggcgctccag tgcccgaaaa atgggacgac tacacatggc
3001 aaacctcatc aaatccatca atcttttaca cctacgggac agctccagcc cggatctcgg
3061 taccgtatgt tggtatttcg aacgcctatt cacactttta cgacggtttt tccaaagtac
3121 cactgaagga ccagtcggca gcactaggtg actcccttta tggtgcagca tctctaaatg
3181 acttcggtat tttggctgtt agagtagtca atgatcacaa cccgaccaag gtcacctcca
3241 aaatcagagt gtatctaaaa cccaaacaca tcagagtctg gtgcccgcgt ccaccgaggc
3301 agctggcgta ctacggccct ggagtggatt acaaggatgg tacgcttaca cccctctcca
3361 ccaaggatct gaccacatat ggattcggac accaaaacaa agcggtgtac actgcaggtt
3421 acaaaatttg caactaccac ttggccactc aggatgattt gcaaaacgca gtgaacgtca
3481 tgtggagtag agacctctta gtcacagaat caagagccca gggcaccgat tcaatcgcaa
3541 ggtgcaattg caacgcaggg gtgtactact gcgagtctag aaggaaatac tacccagtat
3601 ccttcgttgg cccaacgttc cagtacatgg aggctaataa ctattaccca gttaggtacc
3661 agtcccatat gctcattggc catggattcg aatctccagg ggattgtggt ggcatactca
3721 gatgtcacca cggggtgata gggatcatta ctgctggtgg agaagggttg gttgcatttt
3781 cagacattag agacttgtat gcctacgaag aagaagccat ggaacaaggc atcaccaatt
3841 acatagagtc acttggggcc gcatttggaa gtggatttac tcagcagatt agcgacaaaa
3901 taacagagtt gaccaatatg gtgaccagta ccatcactga aaagctactt aagaacttga
3961 tcaagatcat atcctcacta gttattataa ctaggaacta tgaagacacc acaacagtgc
4021 tcgctaccct ggcccttctt gggtgtgatg cttcaccatg gcagtggctt agaaagaaag
4081 catgcgatgt tctggagata ccttatgtca tcaagcaagg tgacagttgg ttgaagaagt
4141 ttactgaagc atgccaacga gctaagggcc tggagtgggt gtcaaacaaa atctcaaaat
4201 tcattgattg gctcaaggag aaaattatcc cacaagctag agataagttg gaatttgtaa
4261 caaaacttag acaactagaa atgctggaaa accaaatctc aactatacac caatcatgcc
4321 ctagtcagga acaccaggaa attctattca ataatgtcag atggttatcc atccagtcta
4381 agaggtttgc ccctctttac gcagtggaag ccaaaagaat acagaaacta gagcatacta
4441 ttaacaacta catacagttc aagagcaaac accgtattga accagtatgt ttgctagtac
4501 atggcagccc cggaacaggt aaatctgtag caaccaacct gattgctaga gccatagctg
4561 aaagagaaaa cacgtccacg tactcgctac ccccggatcc atcacacttc gacggataca
4621 aacaacaggg agtggtgatt atggacgacc tgaatcaaaa cccagatggt gcggacatga
4681 agctgttctg tcagatggta tcaacagtgg agtttatacc acccatggca tccctggagg
4741 agaaaggaat cctgtttact tcaaattacg ttctagcatc cacaaactca agcagaattt
4801 ccccccccac tgtggcacac agtgatgcat tagccaggcg ctttgcgttc gacatggaca
4861 ttcaggtcat gaatgagtat tctagagatg ggaaattgaa catggccatg gctactgaaa
4921 tgtgtaagaa ctgtcaccaa ccagcaaact ttaagagatg ctgtccttta gtgtgtggta
4981 aggcaattca attaatggac aaatcttcca gagttagata cagtattgac cagatcacta
5041 caatgattat caatgagaga aacagaagat ccaacattgg caattgtatg gaggctttgt
5101 ttcaaggccc actccagtat aaagacttga aaattgacat caagacgagt ccccctcctg
5161 aatgtatcaa tgacttgctc caagcagttg actcccagga ggtgagagat tactgtgaga
5221 agaagggttg gatagtcaac atcaccagcc aggttcaaac agaaaggaac atcaacaggg
5281 caatgacaat tctacaagcg gtgacaacct tcgccgcagt ggctggagtt gtctatgtca
5341 tgtataaact gtttgctgga caccagggag catacactgg tttaccaaac aaaaaaccca
5401 acgtgcccac cattcggaca gcaaaggtac aaggaccagg gttcgattac gcagtggcta
5461 tggctaaaag aaacattgtt acagcaacta ctagcaaggg agagttcact atgttaggag
5521 tccacgacaa cgtggctatt ttaccaaccc acgcttcacc tggtgaaagc attgtgatcg
5581 atggcaaaga agcggagatc ttggatgcca aagtgtttga agatcaagca ggaaccaatt
5641 ttgaaatcac tataatcact ctaaagagaa atgaaaagtt cagagacatt agaccacata
5701 tacctactca aatcactgag acaaatgatg gagtcttgat cgtgaacact agcaagtacc
5761 ccaatatgta tgttcctgtc cgtgctgtga ctgaacaggg atatctaaat ctcggtgggc
5821 gccaaactgc tcgtactcta atgtacaact ttccaaccag agcaggacag tgtggtggag
5881 tcatcacatg tactgggaag tcatcgggat gcatgttggt ggacggttca cacgggtttg
5941 cagcggccct gaagcgatca ttattcactc agagtcaagg tgaaatcccg tggatgagac
6001 cttcgaagga cgcgggatat ccaatcataa atgccccgtc caaaaccaag cttgaaccca
6061 gtgctttcca ctatgtgttt gaaggggtga aggaaccagc agtcctcact aaaaacgatc
6121 ccaggcttaa gacagacttt gaggaggcaa ttttctccaa gtacgtgggt aacaaaatta
6181 ctgaagtgga tgagtacatg aaagaggcag tagaccacta tgctggccag ctcatgtcac
6241 tagacatcaa catagaacaa atgtgcttgg aggatgccat gtatggcact gatggtctag
6301 aagcacttga tttgtccacc agtgctggct acccttatgt agcaatggga aagaagaaga
6361 gagacatctt gaacaaacaa accagagaca ctaaggaaat gcaaaaactg ctcgacacat
6421 atggaatcaa cctcccactg gtgacttatg taaaggatga acttagatcc aaaacaaagg
6481 ttgagcaggg gaaatccaga ttaattgaag cttctagttt gaatgactca gtggcaatga
6541 gaatggcttt tgggaaccta tatgctgctt ttcacaaaaa cccaggagtg ataacaggtt
6601 cagcagtggg gtgcgatcca gatttgtttt ggagcaaaat tccggtattg atggaagaga
6661 agctgtttgc ttttgactac acagggtatg atgcatctct cagccctgct tggttcgagg
6721 cactaaagat ggtgcttgag aaaatcggat tcggagacag agttgactac atcgactacc
6781 taaaccactc acaccacctg tacaagaata aaacatactg tgtcaagggc ggtatgccat
6841 ctggctgctc aggcacttca atttttaact caatgattaa caacttgatt atcaggacac
6901 tcttactgaa aacctacaag ggcatagatt tagaccacct aaaaatgatt gcctatggtg
6961 atgatgtaat tgcttcctac ccccatgaag ttgacgctag tctcctagcc caatcaggaa
7021 aagactatgg actaactatg actccagctg acaaatcagc tacatttgaa acagtcacat
7081 gggagaatgt aacattcttg aagagattct tcagggcaga cgagaaatac ccatttctta
7141 ttcatccagt aatgccaatg aaggaaattc atgaatcaat tagatggact aaagatccta
7201 ggaacactca ggatcacgtt cgctctctgt gccttttagc ttggcacaat ggcgaagaag
7261 aatataacaa attcctagct aaaatcagga gtgtgccaat tggaagagct ttattgctcc
7321 cagagtactc aacattgtac cgccgttggc ttgactcatt ttagtaaccc tacctcagtc
7381 gaattggatt gggtcatact gctgtagggg taaatttttc tttaattcgg agg
//