GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS MLFFMLVCG 8359 bp ss-mRNA VRL 28-MAY-1991
DEFINITION Friend murine leukemia virus (F-MuLV) complete RNA genome.
ACCESSION X02794
KEYWORDS DNA polymerase; RNase H; coat protein; env gene;
envelope glycoprotein; gag gene; integrase; long terminal repeat;
pol gene; polyprotein; reverse transcriptase; signal peptide.
SOURCE Friend murine leukemia virus RNA.
ORGANISM Friend murine leukemia virus
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Retroviridae; Oncovirinae; Type C oncovirus group;
Mammalian type C oncoviruses; Murine leukemia viruses.
REFERENCE 1 (bases 5718 to 7860)
AUTHORS Koch,W., Hunsmann,G. and Friedrich,R.W.
TITLE Nucleotide sequence of the envelope gene of Friend murine leukemia
virus
JOURNAL J. Virol. 45, 1-9 (1983)
STANDARD full automatic
REFERENCE 2 (bases 7797 to 8359)
AUTHORS Koch,W., Zimmermann,W., Oliff,A. and Friedrich,R.W.
TITLE Molecular analysis of the envelope gene and long terminal repeat of
Friend mink cell focus-inducing virus: implications for the
functions of these sequences
JOURNAL J. Virol. 49, 828-840 (1984)
STANDARD full automatic
REFERENCE 3 (bases 1 to 8359; 1 to 8359)
AUTHORS Friedrich,R.W.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT Data kindly reviewed (28-AUG-1985) by Friedrich R.
*source: clone=biological clone 201, molecular clone 57; Author's
comment:"The sequence in this entry differs at position 3039 (g
instead of c) from the sequence distributed at the Cold Spring
Harbor RNA Tumor Virus Meeting 1987. This typing error was kindly
pointed out to us by Heinz Ellerbrok."
From EMBL entry REFMLVCG; dated 27-DEC-1990.
FEATURES Location/Qualifiers
LTR 1..86
/function="5' LTR, R region"
mRNA 1..8359
/function="Friend MuLV mRNA and complete RNA genome"
/evidence=EXPERIMENTAL
LTR 87..144
/function="5' LTR, U5 region"
/note="5' LTR, U5 region"
/citation=[2]
LTR 145..163
/function="5''LTR, primer binding site"
/evidence=EXPERIMENTAL
/citation=[2]
CDS 619..2238
/function="gag polyprotein (precursor protein)"
/codon_start=619
/product="Pr65"
/gene=" gag"
/evidence=EXPERIMENTAL
/codon_start=619
CDS 619..5828
/function="gag-pol polyprotein (precursor protein)"
/codon_start=619
/note="A glycosylated gPr200 with an unknown initiation
codon upstream of the AUG initiation of Pr200 is also
present"
/product="Pr200"
/gene="gag, pol"
/codon_start=619
mat_peptide 622..1014
/function="matrix protein"
/codon_start=622
/product="p15"
/gene=" gag"
/evidence=EXPERIMENTAL
mat_peptide 1015..1266
/function="RNA binding protein, phosphoprotein"
/codon_start=1015
/product="pp12"
/gene=" gag"
mat_peptide 1267..2055
/function="capsid protein"
/codon_start=1267
/product="p30"
/gene=" gag"
mat_peptide 2056..2235
/function="RNA binding protein, nucleocapsid protein"
/codon_start=2056
/product="p10"
/gene=" gag"
mat_peptide 2224..2598
/function="protease"
/codon_start=2224
/product="p14"
/gene=" pol"
/evidence=EXPERIMENTAL
exon 2224..5828
/codon_start=2224
/product="protease, reverse transcriptase, RNase H,
integrase"
/gene=" pol"
/evidence=EXPERIMENTAL
mat_peptide 2599..4611
/function="reverse transcriptase (DNA polymerase) and
ribonuclease H"
/codon_start=2599
/note="Reverse transcriptase and ribonuclease H (RNase H)
are separate activities residing on the same polypetide
chain. RNase H is located in the carboxyterminal third of
the molecule."
/product="p80"
/gene=" pol"
/evidence=EXPERIMENTAL
mat_peptide 4612..5825
/function="integration protein"
/codon_start=4612
/product="p46"
/gene=" pol"
/evidence=EXPERIMENTAL
sig_peptide 5778..5879
CDS 5778..7805
/codon_start=5778
/product="viral envelope protein precursor"
/gene=" env"
/codon_start=5778
/citation=[2]
mat_peptide 5880..7216
/function="mature surface glycoprotein"
/codon_start=5880
/product="viral envelope protein gp70"
/gene=" env"
/evidence=EXPERIMENTAL
/citation=[2]
mat_peptide 7217..7744
/function="processed transmembrane protein"
/codon_start=7217
/note="part of TM p15E is further processed to p12E"
/product="viral transmembrane protein, p12E (p12env)"
/gene=" env"
/evidence=EXPERIMENTAL
/citation=[2]
mat_peptide 7217..7802
/function="transmembrane protein"
/codon_start=7217
/product="viral transmembrane protein, p15E (p15env)"
/gene=" env"
/evidence=EXPERIMENTAL
/citation=[2]
LTR 7845..8291
/function="3' LTR, U3 region"
/evidence=EXPERIMENTAL
/citation=[2]
enhancer 7968..8033
/evidence=EXPERIMENTAL
/citation=[2]
enhancer 8034..8107
/evidence=EXPERIMENTAL
/citation=[2]
CAAT_signal 8211..8215
TATA_signal 8261..8269
repeat_unit 8292..8359
/function="elongation of reverse transcription"
/rpt_type=direct
/evidence=EXPERIMENTAL
/citation=[2]
polyA_signal 8338..8343
/citation=[2]
BASE COUNT 2122 a 2427 c 2036 g 1774 t
ORIGIN
1 gcgccagtcc tccgatagac tgagtcgccc gggtacccgt attcccaata aagcctcttg
61 ctgttgcatc cgactcgtgg tctcgctgtt ccttgggagg gtctcctcag agtgattgac
121 tacccgtctc gggggtcttt catttggggg ctcgtccggg atctggagac ccttgcccaa
181 ggaccaccga cccaccaccg ggaggtaagc tggccagcaa ttgatctgtg tctgtccatt
241 gtcccgtgtc tttgattgat tttatgcgcc tgcgtttgta ctagttggcc gactagctct
301 gtacctggcg gacccgtggt ggaactgacg ggttcgagac acccggccgc aaccctggga
361 gacgtcccaa ggacttcggg ggccattttt gtggcccggc cagagtccaa ccatcccgac
421 cgttttggac tctttggtgc acccccctta gaggaggggt atgtggttct ggtaggggac
481 ggaaggctaa aacagtttcc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
541 cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgttt gactgttttt
601 ctgtatttgt ctgaaaacat gggccagact gttaccaccc ccttaagttt gactttagac
661 cactggaagg atgtcgagcg gacagcccac aaccagtcgg tagagatcag gaaaaggcgc
721 tgggttacac tctgctctgc agaatggcca accttcaacg tcggatggcc acgagacggc
781 acttttaacc cagacattat tacacaggtt aagatcaagg tcttctcatc tggcccacat
841 ggacatccgg atcaggtccc ctacatcgtg acctgggaag ctttagcagc agacccccct
901 ccctgggtca aacccttcgt gcaccctaaa cctcctcccc tccttcttcc cccttcagcc
961 ccctctctcc cacctgaacc tccattcccg accccgcctc agtcctccct ctatccggct
1021 ctcacttctc ctttaaacac caaacctagg cctcaagtcc ttcctgatag cggaggacca
1081 ctcattgatc tactcacgga ggaccctccg ccttaccggg acccagggcc atcctcttct
1141 gacgggaacg gcggtagcgg ggaagtggcc cctacagaag gggcccctga ctcttcccca
1201 atggtatccc gcctgcgggg aagaagagaa ccccccgtgg cggattctac tacctctcag
1261 gcgttccccc ttcgccaggg aggaaatgga cagtttcaat actggccatt ttcctcctct
1321 gacctctata actggaaaaa taataacccc tctttctccg aggacccagc taaattgaca
1381 gctttgatcg agtccgttct ccttactcat cagcccactt gggatgactg ccaacagcta
1441 ttagggaccc tgctgacggg agaagaaaaa cagcgagtgc tcctagaggc ccgaaaggcg
1501 gttcgagggg aggacggacg cccaactcag ctgcccaatg acattaatga tgcttttccc
1561 ttggaacgtc ccgactggga ctacaacacc caacgaggta ggaaccacct agtccactat
1621 cgccagttgc tcctagcggg tctccaaaac gcgggcagaa gccccaccaa tttggccaag
1681 gtaaaaggga taacccaggg acctaatgag tctccctcag cctttttaga gagactcaag
1741 gaggcctatc gcagatacac tccttatgac cctgaggacc cagggcaaga aaccaatgtg
1801 gccatgtcat tcatctggca gtccgccccg gatatcgggc gaaagttaga gcggttagaa
1861 gatttaaaga gcaagacctt aggagactta gtgagggaag ctgaaaagat ctttaataag
1921 cgagaaaccc cagaagaaag agaggaacgt attaggagag aaacagagga aaaggaagaa
1981 cgccgtaggg cagaggatga gcagagagaa aaggagaggg accgcagaag acatagagaa
2041 atgagtaagt tgctggctac tgtcattagc gggcagagac aggatagaca gggaggagag
2101 cgaaggaggc cccaactcga ccacgaccag tgtgcctact gcaaagaaaa gggacattgg
2161 gctagagatt gccccaagaa gccaagagga ccccggggac cacgacccca ggcctccctc
2221 ctgaccttag acgattaggg aggtcagggt caggagcccc cccctgaacc caggataacc
2281 ctcaaagtcg gggggcaacc cgtcaccttc ctagtggata ctggggccca gcactccgtg
2341 ctgacccaaa atcctggacc cctaagtgac aagtctgctt gggtccaagg ggctactgga
2401 ggaaagcggt atcgctggac cacggatcgc cgagtgcacc tagccaccgg taaggtcacc
2461 cactctttcc tccatgtacc agactgcccc tatcctctgc taggaagaca tttgctgact
2521 aaactaaaag cccaaattca ctttgaggga tcaggagctc aggttgtggg accaatggga
2581 cagcccctgc aagtgctgac cctaaacata gaagatgagt atcggctaca tgaaacctca
2641 aaagggccag atgtgcctct agggtccaca tggctctctg attttcccca ggcctgggca
2701 gaaaccgggg gcatgggact ggcctttcgc caagctcctc tgatcatatc tctgaaggca
2761 acctctaccc ccgtgtccat aaaacaatac cccatgtcac aagaagccag actggggatc
2821 aagccccaca tacagagact gctggatcag ggaattctag taccctgcca gtcaccctgg
2881 aacacgcccc tgctacccgt taagaaaccg gggactaatg attataggcc tgtccaggat
2941 ctgagagaag tcaacaagcg ggtggaagac atccacccca ccgtgcccaa cccttacaat
3001 ctcttgagcg ggctcccacc gtcccaccag tggtacactg tgcttgattt aaaggatgct
3061 ttcttctgcc tgagactcca ccccaccagt cagtctctct tcgcctttga gtggaaagat
3121 ccagagatgg gaatctcagg acaattaacc tggaccagac tcccacaggg tttcaaaaac
3181 agtcccaccc tgtttgatga agccctgcac agggacctcg cagacttccg gatccagcac
3241 ccagacctga tcctgctcca gtatgtagat gacttgctgc tggccgccac ttctgagctt
3301 gactgtcaac aaggtacgcg ggccctgtta caaaccctag gggacctcgg atatcgggcc
3361 tcggccaaga aagcccaaat ttgccagaaa caggtcaagt atctggggta tcttctaaaa
3421 gagggtcaga gatggctgac tgaggccaga aaagagactg tgatggggca gcctactccg
3481 aagacccctc gacaactaag ggagttccta gggacggcag gcctctgtcg cctctggatc
3541 cctgggtttg cagaaatggc agcccccttg taccctctca ccaaaacggg gactctgttt
3601 aagtggggcc cagaccagca gaaggcctac caagagatca agcaggccct cttaactgcc
3661 cctgccctgg gattgccgga cttgactaag cccttcgaac tttttgttga cgagaagcag
3721 ggctacgcca aaggtgtcct gacgcaaaaa ctggggcctt ggcgtcggcc ggtggcctac
3781 ctgtccaaaa agctagaccc agtggcagct gggtggcccc cttgcctacg gatggtggca
3841 gccattgccg ttctgaccaa agacgttggc aaactcacca tgggacagcc actagtcatt
3901 ctggcccccc atgcagtaga ggcattagtt aagcaacccc ctgatcgctg gctctccaac
3961 gcccgaatga ctcactacca ggctctgctt ctggacacgg accgagtcca gttcggacca
4021 atagtggccc taaacccagc tacgctgctc cctctacccg aggaggggct gcaacatgac
4081 tgccttgaca tcttggctga agcccacgga actagaccag atcttacgga ccagcctctc
4141 ccagacgctg accacacctg gtacacagat gggagcagct tcctgcaaga ggggcagcgc
4201 agggccggag cagcagtaac caccgagacc gaggtaatct gggccaaagc actgccagcc
4261 gggacgtcgg cccaaagagc tgaactgata gcgctcaccc aagccttaaa aatggcagca
4321 ggtaagaagc tgaatgttta cactgatagc cgttatgctt ttgccactgc ccatattcac
4381 ggagaaatat atagaaggcg cggtttgctc acatcagaag gaaaagagat caaaaataag
4441 gacgagatct tggccctact gaaggctctc ttcctgccca aaagacttag cataattcat
4501 tgcccgggac atcaaaaggg aaaccacgca gaggcaaggg gcaacaggat ggccgaccaa
4561 gcggcccgag aagtagccac tagagaaact ccagagactt ccacacttct gatagaaaat
4621 tcagccccct atactcgtga acattttcac tatacggtga ctgacataaa agatctgact
4681 aaactagggg ccacttatga cgatgcaaag aagtgttggg tttatcaggg aaagcctgta
4741 atgcctgatc aattcacctt tgaactatta gattttcttc atcaattgac ccacctcagt
4801 ttctcaaaaa caaaggctct tctagaaagg agctacagtc cttcttacat gctgaaccgg
4861 gatcgaacgc tcaaagacat cactgagact tgcaaagcct gtgcacaggt caatgccagc
4921 aagtctgccg tcaagcaagg gactagagtt cgggggcacc gacccggcac ccactgggaa
4981 attgatttca ctgaagtaaa acctggcctg tatgggtata aatatctctt agtttttgta
5041 gacactttct ctggatgggt agaagctttc ccaaccaaga aagaaactgc caaagttgta
5101 accaagaagc tactagaaga aatcttccct agattcggca tgccacaggt attgggaacc
5161 gacaatgggc ctgccttcgt ctccaaggta agtcagacag tagccgattt attgggggtt
5221 gattggaaac tacattgtgc ttacagaccc cagagttcag gtcaggtaga aagaatgaat
5281 aggacaatca aggagacttt aactaaattg acgcttgcaa ctggctctag ggactgggtg
5341 ctcctgcttc cccttgccct gtatcgagcc cgcaacacgc cgggccccca cggtctcacc
5401 ccatatgaaa tcttatatgg ggcacccccg ccccttgtaa acttccctga tcctgacatg
5461 gcaaaggtta ctcataaccc ctctctccaa gctcatttac aggcactcta cctggtccag
5521 cacgaagtct ggagaccgtt ggcggcagct taccaagagc aactagaccg gccggtagta
5581 cctcaccctt tccgggtcgg tgacacagtg tgggtccgca gacaccaaac taaaaatcta
5641 gaaccccgct ggaaaggacc ctataccgtc ctactgacta cccccaccgc tctcaaagta
5701 gacggcattg cagcgtggat ccacgctgcc cacgtaaagg ctgccgacac caggattgag
5761 ccaccagcag aatcgacatg gcgtgttcaa cgctcccaaa atcccctaaa gataagattg
5821 acccgcggga cctcctaatc cccttaattc tcttcctgtc tctcaaaggg gccagatccg
5881 cagcacccgg ctccagccct caccaggtct acaacattac ctgggaagtg accaatgggg
5941 atcgggagac agtatgggca atatcaggca accaccctct gtggacttgg tggccagtcc
6001 tcaccccaga tttgtgtatg ttagctctca gtgggccgcc ccactggggg ctagagtatc
6061 aggcccccta ttcctcgccc ccggggcccc cttgttgctc agggagcagc gggagcagtg
6121 caggctgttc cagagactgc gacgagccct tgacctccct cacccctcgg tgcaacactg
6181 cctggaacag acttaagcta gaccaggtaa ctcataaatc aagtgaggga ttttatgtct
6241 gccccgggtc acatcgcccc cgggaagcca agtcctgtgg aggtccagac tccttctact
6301 gtgcctcttg gggctgcgag acaaccggta gagtatactg gaagccctcc tcctcttggg
6361 actacatcac agtggacaac aatctcacca ctagccaggc tgtccaggta tgcaaagaca
6421 ataagtggtg caatcccttg gctatccagt ttacaaacgc cgggaaacag gtcacctcat
6481 ggacaactgg acactattgg ggtctacgtc tttatgtctc tgggcgggac ccggggctta
6541 ctttcgggat ccgactcaga tatcaaaatc taggacctcg ggtcccgata ggaccgaacc
6601 ccgtcctggc agaccaactt tcgctcccgc gacctaatcc cctacccaaa cctgccaagt
6661 ctccccccgc ctctaattcg actcccacat tgatttcccc gtcccccact cccactcagc
6721 ccccgccagc aggaacggga gacaggttac taaatctagt acagggagct taccaggcac
6781 tcaaccttac caaccctgat aaaactcaag agtgctggtt atgcctagtg tctggacccc
6841 cctattacga aggggttgcc gtcctaggta cttattccaa ccatacctct gccccagcta
6901 actgctccgt ggcctcccaa cacaagttga ccctgtccga agtgactgga cggggactct
6961 gcataggaac agtcccaaaa actcaccagg ccctgtgcaa cactaccctt aagatagaca
7021 aagggtctta ctatctagtt gcccccacag gaactacgtg ggcatgtaac actggactca
7081 ctccatgcct atctgccacc gtgcttaatc gcaccactga ctattgcgtt ctcgtagagt
7141 tatggcccag ggtcacctac catcctccca gttacgtcta tagccagttt gaaaaatcct
7201 atagacataa aagagaacca gtgtccttaa ccttggccct attattaggt gggctaacta
7261 tgggtggcat cgccgcggga gtagggacag gaactaccgc cctggtcgcc acccagcagt
7321 tccagcagct ccatgctgcc gtacaagatg atctcaaaga agtcgaaaag tcaattacta
7381 acctagaaaa gtctcttact tcgttgtctg aggttgtgct gcagaatcga cgaggcctag
7441 acctgttgtt cctaaaagaa ggaggactgt gtgctgccct aaaagaagaa tgttgtttct
7501 atgctgacca tacaggccta gtaagagata gtatggccaa attaagagag agactcactc
7561 agagacaaaa actatttgag tcgagccaag gatggttcga aggattgttt aacagatccc
7621 cctggtttac cacgttaata tccaccatca tggggcctct cattatactc ctactaattc
7681 tgctttttgg accctgcatt cttaatcgat tagttcaatt tgttaaagac aggatctcag
7741 tagtccaggc tttagtcctg actcaacaat accaccagct aaaaccacta gaatacgagc
7801 catgataaat aaaagatttt atttagtttc cagaaaaagg ggggaatgaa agaccccacc
7861 aagttgctta gcctgatagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
7921 ccaagaatag agaagttcag atcaaggtca ggtacacgaa aacagctaac gttgggccaa
7981 acaggatatc tgtggtaagc agtttcggcc ccggcccggg gccaagaaca gatacgctgg
8041 gccaaacagg atatctgtgg taagcagttt cggcccggtc ggccccggcc cgaggccaag
8101 aacggatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
8161 tccaggctcc cccaaggacc tgaaatgacc ctgtgcctta tttgaattaa ccaatcagcc
8221 cgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
8281 cctcactcgg cgcgccagtc ctccgataga ctgagtcgcc cgggtacccg tattcccaat
8341 aaagcctctt gctgttgca
//