GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS RATHLURR 2749 bp ds-DNA ROD 28-MAY-1991
DEFINITION Rat hepatic lipase gene upstream regulatory region and exon 1
ACCESSION X17367
KEYWORDS cAMP response element; enhancer; estrogen regulatory element;
glucocorticoid response element; hepatic lipase; lipase;
sterol response element; thyroid response element.
SOURCE Rattus norvegicus DNA.
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2749)
AUTHORS Bensadoun,A.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2749)
AUTHORS Sensel,M.G., Legrand-Lorans,A., Wang,M.E. and Bensadoun,A.
TITLE Isolation and characterization of clones for the rat hepatic lipase
gene upstream regulatory region
JOURNAL Biochim. Biophys. Acta 1048, 297-302 (1990)
STANDARD full automatic
COMMENT *source: tissue=liver; library=Charon 4A; See <X17366> for cDNA
sequence.
From EMBL entry RNHLURR; dated 04-MAY-1990.
FEATURES Location/Qualifiers
misc_feature 113..120
/note="OCT-1 site"
misc_feature 237..245
/note="enhancer binding site"
misc_feature 269..276
/note="sterol response element"
misc_feature 445..452
/note="AP-2 site"
misc_feature complement(464..469)
/note="glucocorticoid response element"
misc_feature 710..717
/note="sterol response element"
misc_feature 996..1001
/note="glucocorticoid response element"
misc_feature 1064..1069
/note="glucocorticoid response element"
misc_feature complement(1071..1076)
/note="glucocorticoid response element"
misc_feature 1212..1224
/note="estrogen regulatory element"
misc_feature 1365..1370
/note="glucocorticoid response element"
misc_feature 1501..1515
/note="glucocorticoid response element"
misc_feature 1698..1710
/note="thyroid response element"
misc_feature 1709..1716
/note="AP-2 site"
misc_feature 1781..1788
/note="cAMP response element"
misc_feature 1824..1830
/note="sterol response element"
misc_feature 1890..1898
/note="AP-2 site"
misc_feature 1938..1953
/note="thyroid response element"
misc_feature 1999..2022
/note="apolipoprotein CIII promoter element"
misc_feature 2039..2046
/note="sterol response element"
promoter 2096..2103
/note="CAAT box"
promoter 2259..2264
/note="TATA box"
mRNA 2288..2422
/note="exon 1"
CDS 2338..2422
/note="hepatic lipase (AA 1-28) (2422 is 1st base in
codon)"
/codon_start=2338
intron 2423..>2749
/note="intron I"
misc_feature 2572..2583
/note="estrogen response element"
misc_feature 2615..2622
/note="cAMP response element"
misc_feature 2661..2673
/note="estrogen regulatory element"
misc_feature 2734..2741
/note="OCT-1 site"
BASE COUNT 695 a 734 c 585 g 735 t
ORIGIN
1 ctgcagagtg aggtaggggt tgggggtgat tgctacagca atcagagaag aagagaggag
61 agcagggtga tagaagaggg acccacggcc aaatggtctt aggggtttga agatttgaat
121 gcttagtgtg aaaaaagtag aagtttcaga tcctgcttaa ccatgcaaaa caggaaggag
181 ataccccgaa tccataaacg cagtccttct tcaggaatcg ggatagcccc cacagctgtg
241 gaaggaagct cagccacaga gcacacaggt tcggtgatgg gttcgagacc atcacagctg
301 cgtgcacata tgccatggga ggcaaacgct ggcatccatt gtcttctcaa tcaccctcca
361 tcttgagtct ctccctggga cccacagctt ctaattatgc aaggctggct tccagctagg
421 ctcggtgatc agcctggtcc tgcctcccag gcactggatt gcaagaacat ggctggctct
481 tttcaaatgt ggctttaggg gatcaaattc aagtccttgt gcttgcattc caagcgcttt
541 cctccctgag ctgtctcccc agatctttca gagagctttt aagtcagtca ttagcagaca
601 gcgattggca ctttacctaa ctgtctacta accacttcta ccatctactg gagtgtataa
661 gtgccgaact cagacgccac agagcaaaat ctgagttgct cctcatcccc accgaactag
721 catagctagt tgcacccata gcttctctgt ctcggttgat ggtaacccta ttcatctatt
781 ggctgaaaac aaaaccctcg gagtcattct ccactctctt ctcgcctcct ccctcccgtc
841 aatccaagga gtctagtagg ttcactccca aaaacccagc ccagccccca tccatgattc
901 catcacacca gccaccatca aagcctcctg gctcagtcac tcttcagtca gctctcctct
961 tcagccttgc tcacagaccc agttatttgc tcttctgttc tgcatgtcca agtcttttat
1021 gtaaaagcct cggatgggtc tccctgctca gtagccctct ccctgtcctt agaacacacc
1081 aaccgtgttc caacctcagg acctttgccc taactgttcg ttctgaccat tccattcaaa
1141 accaccgcct tctccgttat ccctgctatt tctttatttt tactttattt tttgatacca
1201 ggcatcatgt agctcaggct gactttgact cctgacactc ttgcctcctc ctgagtgctg
1261 gggctatagg tgtgtgccac caaagccact tattagggat cttgctcagg cttcaggcct
1321 gctaggaaag cactctacca actgagctac attcccagcc aaactgttct catgctgtat
1381 ctctgctttc taccatataa tccttttcat gcacacatcc cccgtggtag taaactccaa
1441 aggactgggt acttgtccgc tttattcacc taactatctc aagagccaac aacagcgcct
1501 ggtacacagt ggtgttcaac acataacatt tatttgtcca gggcgtccat actaaataaa
1561 taaataaaat ttaaatgaca ctagagagcc aagaactctt gcaagccctg cacatcacag
1621 cctcttccct gtatgctttt gctgaatgac cttgcttcct agccctctac tctcgactgg
1681 acattaatta acccagtcag tgagtttatc ctggggtatc tctcaacagg gccctcagca
1741 agactctggt cgttgcttct cacccagttc cttcttaacc tgagctcacc aggttccagc
1801 tcctctttca gctatagaga tgctacccca ccacgattat actgcagatg cccaaaacat
1861 ggctgacctc aacaggaagc agagtccctg ggggtggggg cttttacctc tctttggggc
1921 caacatgcca ctagaaagac caggaccatg acgccacctc ccctctgagt gctttatagg
1981 ataacacagg caacaataca gaggtcacct agctgacctt tgatcaaatg tcaaaacaca
2041 ccgcactaac tttcaactga ttgattctga actatgagct tggctcaaaa ggaaagaagg
2101 cctgatgagg tgttttattt cccaaattta tcaagattaa aaagtgcttc tgtgggtcaa
2161 tctagatatt gctagatttg gattggagga ggcaaagaaa gtgttcaaat actgggtaac
2221 atgttttagg gttaataatt aatggggcct tccacaacta aataccaaga agcattctgg
2281 tctctttagt tctagacatt aacaagaaga cctcaggccc cgggtaagac gagagacatg
2341 ggaaatcacc tccaaatctc cgtttccctg gtgctgtgca tctttatcca gtcaagtgcc
2401 tgtggacaag gcgtgggaac aggtacgtgt ttgccttttc tccagaaatg gccgtggatt
2461 tctcttcttg actgtgttgc agagattggc gatgtgggat ggaatctgct tcagagctcg
2521 caaagtggct ctgcaagtag ctcccaaggc acccagagct gtgtagcact ttgtaaaagg
2581 gaccgcattt tcaaaaagct gctctgagag tagatgacgt aaataaagcc cttgtaacag
2641 gcagtgcagt ctcctgcagg tgtcatgtag accctgggag agttttgtca gcacacaatg
2701 atctgaaagt cttgggaatc ttccctatgg ttcatttgaa ttcgagctc
//