GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS CHKASMB 11007 bp ds-DNA VRT 28-MAY-1991
DEFINITION chicken alpha-smooth muscle actin gene.
ACCESSION D00041 N00041
KEYWORDS alpha-smooth muscle actin.
SOURCE Gallus gallus DNA.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 11007)
AUTHORS Carroll,S.L., Bergsma,D.J. and Schwartz,R.J.
TITLE Structure and complete nucleotide sequence of the chicken
alpha-smooth muscle (aortic) actin gene: An actin gene which
produced multiple messenger RNAs
JOURNAL J. Biol. Chem. 261, 8965-8976 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P08023; ACTA$CHICK.
The complete nucleotide sequence of chicken alpha smooth muscle
actin gene was determined. This gene was composed of 8 IVSs and 9
exons. The transcription was initiated at one site and was
terminated at 4 sites, probably as a result of the utilization of
alternative polyadenylation signals.
From EMBL entry GGASMA; dated 26-APR-1990.
FEATURES Location/Qualifiers
precursor_RNA 1014..1058
/note="alpha-smooth muscle actin exon 1, untranslated
sequence"
precursor_RNA 3314..3356
/note="exon 2, untranslated sequence"
CDS 3357..3485
/note="exon 2, translated sequence"
/codon_start=3357
CDS 5091..5219
/note="exon 3"
/codon_start=5091
CDS 5664..5774
/note="exon 4"
/codon_start=5664
CDS 6273..6357
/note="exon 5"
/codon_start=6273
CDS 6440..6601
/note="exon 6"
/codon_start=6440
CDS 6833..7024
/note="exon 7"
/codon_start=6833
CDS 7994..8175
/note="exon 8"
/codon_start=7994
CDS 9005..9148
/note="exon 9"
/codon_start=9005
BASE COUNT 2980 a 2227 c 2355 g 3445 t
ORIGIN
1 gaattcatgg gctttttgaa tttgtagtgg tttgagatgg agtttggaga tgctaatttc
61 tgatctctag tagtagttca agggcaatgt attgttactg tgaaagggct gctcatgaga
121 cacagtctgc ctagagaaca gctggctgca gccaaataaa tccagtcctc tgaaaatagc
181 tcatacattg agaacctttg ctttagttgc taaaaatatg ctcagggcaa agctagctag
241 aggttatgaa attcagcaac tttattatga atgttttgag ataggagttt acaacttgtg
301 tccatcagtg gaattgacac taggatgaag cttgtccaca gttcctagtg ctttggaaat
361 aaactgatgg agacaggata ttgattgtca cccattacag gctaggggca ccataacaac
421 ctgttagcag aacgtttaca cagccttcaa agaccctacc atgaacccta tgcaacagca
481 ggtacttctt ttagtatccc caagtgcaga ccttttaagt gaatttgtgg caaaattcag
541 tagctgttta gcttgccgaa agtattctca ttgctttggt ccaaatcttt aacaaatgca
601 aagtgtctcc ttaaaaacac tttccctatt acaaatgact gctctttcag ttttcactct
661 gcctcttgga tgttcctgtg aaggccaggg cctctctctc ttgtttgaac gtgtgctctt
721 cctgacagag ggtgtctgtc ccaggcacgc ttttcttgct gcattttagc aagttctgca
781 gtgtttatct tacacagctg aaagtctcct cctgtttcat gagctctgcg ttggaatgca
841 gtggaaggga ctgagggcct gtcgacccag attagaggtt tttgtaataa ggtccctata
901 tggttttgtt agagacttcg gctctgtctc tctcatctct gctccttgtt tgggaggctg
961 gtgggaggag aagagctgaa ggggctatat aaccctggtg cttttggata cacagtgcac
1021 catcccagag ctgctatccc cagccaagca ctgtcagggt aagtggcact gaaccaatag
1081 tgggatttat agttttctgg atgactttaa ttaagtaatg tcacatggaa gctattcagg
1141 aggatgtact gctatgctgc agtttgctta ggcattactt actagaactg aattggtaaa
1201 atactttcaa tgtctacact gagttgtatt tgttttaaag cacttttgaa tgggaaatac
1261 gtctgatgat tttgccgatt ccaccaacac tccaacggta atataaagac acagactgtt
1321 taatggcaca gctggaattt aagagaacct gtgtgcccct gtggagttag ctttggacag
1381 aacagagttc ctgaatgggt gaatttgcac actgtgtagt ggtttctcag cagctttgct
1441 tcagtgctct caaaatcagc ttaaattgac gtaagtgttt tggagtgtga ctgcaagaag
1501 agctggaaga tgcaaaatag cagtatctaa tcagatgcaa tgaggatgca tgtgtattca
1561 ttgctgtctc gatagatatg aaagctgtgg tctgcaaaac gcccaatatt ttattaaaga
1621 tcacattata cacagagttc cttgtgaggc tggagttgtt ctcctgatag catgctgtag
1681 aggctgggga agtgattggt tgtctttcag tgtaaagcag gtagaagtaa gaggctaaat
1741 actgtattaa ttgctggggt gaatatgtcc tttattctgc agtgtgagtg acttttgctg
1801 ctggaggatg ttactactgc atgccatggc agtccttgag ctgtaactca ctccttggaa
1861 gagagtgtcc tgcctgaatg atttagcttt gatttttagc tttttgtgct ctattactaa
1921 atatggtttt cattagagtc ctccaagcta gaaatgcagc cttttccagc tccctcctct
1981 cccctccccc aagtgatttt tggcattgca ttctctgcat tggtttgagc aaaccccctg
2041 acctcgaact ctgttccaaa aacagacggt tggaaagcat atttcctaat taggaaatgg
2101 tttctctaaa ccactctgtt cattcatgtt agataacaat tgtactccat agactaaatg
2161 cttaaatata aagagcctgt tttcccaaaa gtttaagaaa gtgcgaaaaa ttgcaaccta
2221 ctttcctttt ctggtaataa tgacttaata tctggagtac atcaacgtgg gatttccctc
2281 tccatgcctt ctcctggcag ctactgtatc catcgagaac tgcagcctga gaagcagtcc
2341 acagctgcgt gctcgtggct gtgaagggtc tgcagtgaga ggcgtttggg ggaggctgtc
2401 cctcctaggt ccatctatgg tggaggctga agcgttgcct catgctccca tgctcaatca
2461 gccatggctc tcactgacgc gcactgccgc ttcgacgtgc acgccagcag gcccatggca
2521 gcaggttttg atcgttcgcg aggagccagc tgggctgctg gatgacagcc tgtctcgctt
2581 tggctgttaa cacattgcaa tttgttgacc tctgcatgga agtccaggct cccagctagt
2641 cgagtgattc cctaacacac tataaattgt gggcaaatag ttctcctcga gtgctggtat
2701 tcggggcttg tttccgtaat tgactttaat acaaaccctt taaagcattt ttattaccct
2761 tgttatcttc ctgttgcctg aggagaaaaa caatttctgt tttagtgaag cagggagcca
2821 gcataaatta ctttgtcatt ctacaaatgc agcttattag ctggtttgaa atgatgatgg
2881 agcacacact atggacagtt tcaaaacaca tgctgtcctt gattgcattt taaagtcagg
2941 atatcatctt tctacgtgca ccagtcttgt caggatgata gaggcagggg acatcatact
3001 gaatctgatg caaagagacc tttgtttttg cagctgtcag tccagcagtc ttctttatct
3061 cccacctacg cctcagtggt ggatttccgt ggccgaattt agataaacat tcgctgtctc
3121 aaagctgtaa tgatctgtct ttccatgcag caggactgga atagttccat ggagtacttt
3181 gaattatgtc tggtgcatac agccttcctg cctatcagtt ccttttatac cgcattctct
3241 gtcttacagg gtggttctgg tacctcactt tgttgttttt ttttcaatta ttcttttctt
3301 gctgtttcca tagggctgtt ttctgcttgg agcacctgag gacattgaca tagaagatgt
3361 gtgaggagga ggacagcact gcccttgttt gtgacaatgg ctcagggctc tgtaaagctg
3421 gctttgccgg ggatgatgct ccaagagcag ttttcccttc catcgtgggt cgtcccaggc
3481 accaggtgat gaaatgctta agttattcca tactgggctt ggagacatgc cccattccca
3541 actctccatc cccgagctac cttttcctgc atctgaggct aacccagccc tagatagtct
3601 gtgtaatgtg gtatttatag gcaaaatgtg tccagcagtg cactcacctg agtcaaagca
3661 gatctatagc cttgtgaaag gaatcctcaa gttttgcact tccttctatg ctctcttctc
3721 cctgtacttt gcgtcaccca gagaacttgg tgagtacaga cagtttaaaa gcaaacaatc
3781 ccagagaagt atctggactt tttgactgcc ctaaaactga tgatagatga tgtgcaggga
3841 actaagggag gaaggagaag ctgtcagtct gtttcccatc ttcttctttt cactgtgtca
3901 cagacagaca cctctctggg gtttctaggg ctgctttttt tggttttggg tttttttttt
3961 aatttttttt ttagctgcaa gctttacatt tgaacatatg atatattctc atcgttttta
4021 acagtttgca aagtgtattt attagctgag ttgttaaata caaggttgct gagccatatc
4081 aacattagtg agatctagag cagcttagct aggccataac aaactaagct ggctgctggg
4141 ttttaaaatc cacctaattt cttctgtgtc acctcaggac gtgttgaaat gaggagaggt
4201 ggactggctg taaggtgtgg cacttaatgc acccagctaa taagaaaaca agccattgag
4261 ggcaagtgaa atatgtgcca aaaggtatag ccccaggagg tctaattgaa gcctctgaac
4321 attaccataa tttaaaaata tatatattca agtgtcatct tccatagaca ggacgaatta
4381 cattaattgc tcaaaaataa atcatagggt cacagtagaa aaatatacag cagattctta
4441 aaatctcttt tttcctgatc atgtattatg gaagacatta gacctctgaa ggaaggatca
4501 tcccatcata aactcaaagt ctggacctgg ttgtttccaa ggtacaactt gttagagaag
4561 ccatgttttg gagcataaaa tgacttaagt tcaggtaaga gtgttatgta gtcaaagcat
4621 cctacaggat actatttttt aacagtattt ctaacttaaa gcatagagac tactgtatat
4681 ctggagtagt ttccaacttc tagcccatag acttggaaaa tctgaattgt gtattgctgc
4741 atcacggccc tggttagatg gcacaatgtc cccatctggt ttcctatgct tcagcagaca
4801 ggtcatccct aagtaacaaa gaacattaaa acaagcctat cattctactt catcttgatg
4861 agtttgacat tgtacttctt gtgtgtgcat gcaactgaat atcatacaaa aaaattgtat
4921 ttgtttgctg tttacttaac tgtactggaa aaatgattaa ttctccagtc attagtattt
4981 ataaagtgtt ttgtaattag tgtcactggc aactgaattt atctcaggtg gtgtaactac
5041 tttgtgacac ctctcctttg aaagctaact ctatttgtgt tattttatag ggtgtgatgg
5101 ttggtatggg tcaaaaagac agctatgtag gtgatgaggc tcaaagcaag agaggaatcc
5161 tgaccttgaa ataccccata gaacatggca tcattaggaa ctgggatgac atggagaagg
5221 tacgtcagtg ttgtaataat gtgcatcgct ggtagacaat tccttgaacc gaatgtttaa
5281 cacattactt ggtacatttt agatgaaagt gttgtgctgt caggtgtggt ttaatgagaa
5341 gcttgttagc agcaagctgg aattaagtct atgcagctct ctctcctatt gcaagattat
5401 atatattttc attaatttgt gtaccctttg aagaagtcgt ttatcattaa cagtgatatt
5461 tctaacctga ggatagagtg tttccttctc cagttacctc ggttatttct acgaaggcaa
5521 taagaacaat tgcatgtgaa acgtttctca ccggtctcta aagtgctata agcagagtgc
5581 tggagcatta aaccagtctg aaagtcattc tgagctgcta gttgatggta tttttctcct
5641 ttgccttctg ccattgtcct cagatctggc atcactcctt ctataatgag ctccgtgttg
5701 cacctgagga gcacccaact ctgctgactg aagcaccact gaatcccaaa gccaatcgag
5761 agaaaatgac ccaggtaatc tcaccctgaa gctatggaat aattccaagt ttgtggattc
5821 agcaggatgt gaacatcatc atttcagtta gtcatgacaa cttgattgtt ctgcagtgtg
5881 tatctcttag taagggaaga tagaaagtgc acagtgactg tgtgtcagat ttctatgtta
5941 ctacatttat ttggaaaagt ctgtgtatca gttttggatg tgagaactct ggtctgcccc
6001 aagtgttgtc actttcatct ttctgtgtgt gtgtgtgtgt ccaggcctac tacaagatga
6061 tctttaatgt gtctgtatta atacattgta gatgtagatt ggatacggag gggtcagaaa
6121 ggacaaacgt ttgatttaaa tgtattttcc ttccttgtta ttaccaacca tcactaaata
6181 caaaaatctt gtattttccc tttcctttgc tgtaatgata caactaaaca aaaatcccta
6241 ctgctaagct aattgtgtta attgcatttc agattatgtt tgagactttc aatgtgccag
6301 ccatgtatgt agctattcaa gctgttctgt ccctgtatgc ttctgggcgt actacaggta
6361 ggcctctgga ggtggcactg gtgttcaaaa cagttgtgtg tgagcacctg agcctgaatc
6421 atgtgttatc ttctcaaagg gattgtgctt gactctgggg atggtgtcac ccacaacgtg
6481 cccatttatg aaggctacgc tttgccacat gccatcatgc gtctggacct ggctggccgt
6541 gacctgacag actacctcat gaagatcctg agcgaacgtg gctactcctt tgtgaccact
6601 ggtaagggca tgctgcatag ctggaggagg ggagttgttt aatgtgcaat gtttctagtg
6661 cccttagaag gaatagcagt ggttctgcag ttgttttcat taacaagaaa gaaatcaaca
6721 ggaaaatact ttctctgcag gattggcaga gaggatctgt gtaaaatgga gaggatgttg
6781 gagaatgaaa atgtggctga gtgccattct aaaaactgaa tcttctcaac agcggagcgt
6841 gaaattgtcc gtgacatcaa ggagaaactg tgttatgtgg ccctggactt tgaaaacgag
6901 atggccactg ctgcctcttc ctcctctctg gaaaaaagct atgagcttcc tgatggccag
6961 gtcatcacca ttggaaatga acggttccgc tgcccagaga ctctcttcca gccatctttc
7021 attggtaggt tgaatgacta ttgtgctttg gtaagctgct cgtaaatacc aggtctctat
7081 ttccttctga ttgtatccca ctgctggctc atttagatgt atacgcaagt atgtcccagt
7141 gcctattata aaataatctt tcagaaccga gcactattaa tacactggga ggaactttat
7201 gtcaaacagt aaagcaaaat gctttcccaa aataaactcc gagtgtttat ttctggcttt
7261 gagcccatga cattgtgtat attatgtatt agttactatg aaaattctgg ctgcagaaac
7321 acttttctaa atggtatgag ggttatagtc tgaatgtgct tgcagggagg ggttaaaaaa
7381 caagcgaaca aaaagcacta cttttattct actgaaagaa ctgccccaaa tgtgaatcca
7441 gagtgcatca catttgcaga gcagatcaat ggtcatggag gcaagcttgc agcaaggtca
7501 cgtaaaacct agtttgatgg aatatcagct cataaataga tgaagtagct gcatacttag
7561 ttaaaggtat tgggacatat tttgactatt tttttctcta agctggctca gtttaaaaag
7621 ggagaaaaaa aatagaggga aaaaaaaaaa gtaagaacaa gaaaacaaat gattccagga
7681 ccatatacga attcttcgga catatgaggt tgtctgcaaa agcctaccgg aaactgctag
7741 ctactccata cccctggatt ttctttttaa cgaggagtgt gagtgtgtag atgggggtgg
7801 ggaaagaggc tgtgcccaaa ggagcttcta agtaaataat tcaaacagca gcagaatgac
7861 tttcgtattt ctgcagcctt gaagactgtt gtcttccaaa gacaaactgc agttcacatt
7921 gtctgttcag tgttaacaat tttaagtctc tgtatggagt tgcttcctca gtgttctgct
7981 gtcatttctg taggcatgga gtccgctggc attcatgaaa ccacttacaa cagcatcatg
8041 aagtgtgaca tagacatcag aaaggatctt tatgccaaca atgtgctttc tggaggcact
8101 acaatgtacc caggcattgc agacagaatg caaaaggaaa taactgctct agctcccagc
8161 accatgaaga tcaaggtaca gtgcaataat ttgtaaacta ttgagggaac atgtaaaaat
8221 cctagaaatc taacaaagaa tatcaactct ttacaaagtg ctttgtacat attttcagtc
8281 aagcctgcct gttctactaa atcatcccct ggtaactgca cgagtcaccc tggctgctaa
8341 aagaagacgg ggccttctct cctctcactt cctttctcat gctgtgttct gcaaactgac
8401 ctagcaaggc caaagaagaa tagcatggaa tcagcaacgt tcatttttgt agagctgcag
8461 tttcttcagc aatttatggc taatggcatt caggtgtagg aaatgcagct tctgtccttg
8521 gctcacaagt taagaaaaag attctaacaa gttattcatc cttctgctgc tgcatctctc
8581 cccttccttg ctttgcaaac attttgtcag ctgtactgat aggaacattc catcggtcat
8641 tcattcttcc aagccccccc gggctaccat tctatgtttg tctcatgcta tgagagtggc
8701 tgcctctcct tggtctcttt tgtggttatg aatgcacacg cacacacaca cacacacaca
8761 cacacacaca cacacacaca cacgttgttg tgttgggcag actgattcaa ccagaggaac
8821 tgagggtgta ggctcaggct caggcaacag ctttgtaact tcatggcttg ctgcaatggc
8881 taactgcaag acactagtgc acatgtacag tgttttcact aatacaaagt atcactgctt
8941 taatagttct gtataacaaa ttcgtagctt aacgttgcat ttaatggttt tatttcttaa
9001 acagatcatt gctcctcctg aacgtaagta ctccgtctgg attggaggct ctattcttgc
9061 ctccctctcc accttccagc agatgtggat cagcaaacag gaatatgatg aggctggccc
9121 atccatcgtt caccgcaaat gcttttaaat cgctttatac ttctatgtct ttttagcata
9181 ttactgtgaa tgtattcagg aaaatacatt cttaaaattt cattccataa atctttcatc
9241 gtaatggctg gttaattgga tacggtgtat tttttggaat aaattttaaa tatgcaaatg
9301 aaattgctgc tcctagttta gagatgcagt tgtggagttg ccattacagc tggtgcgaag
9361 ctccatctcg tggcgtaaat ttgctactgc gctgtgggcg gagaagtggt acctgttgcc
9421 cagtggtatc aactccagaa aacgctcagc ctcccagcct gcacggtgcc agcgttcagc
9481 ctcggtatgc atttcagtct gcaagcagaa ctagtcatgg tgttcccaaa gttctgggca
9541 gcagcagagg ggcacagcat ctgcttggta ccaggaggag cacgtgcact ctgctttgca
9601 tctgctctat ccagaaatat tatctgtcca catcacttct cagttcacag ctaccgtgcc
9661 tggaaaaggc acttcttata ccctacgacc cagaagtgta caatgctttc tatactttca
9721 tgttgtttct tttcgtttgt ttctattaaa aataaaacct ctcccttctg ggagcatatc
9781 tgagtgctga tattagcttg aataggtgac tttgctatta tgtgtcctct gaatgagtgt
9841 agcttatgtt gttgttgtga ttttcatcaa agtttaaggg taattaagct ggcttaatac
9901 aattgctctt cagaagtttg tttgtttgtt tgtttttctg gaggggagga tgtaatgtag
9961 tcagagcaga taaaatgttt ttagacaaag ttctttttct atgacttttc tgattccgct
10021 tattgtctta aatacattga gactgatttt taagagaagt atacgcataa acgttgaagc
10081 caatactgct gtcttagaag tatatatata tatatataaa atacgtttat gaccaaagaa
10141 ttaattgaga cagacctgaa aaaggtcagt tctaaggact ttgctgcaat ccatattcat
10201 tagagttcag gatgaagaat gctatttatt tttgttactt attttcctgc cttaaactca
10261 actgtttact tacctcacct acctgttact ccttgccttt gaacctgtaa gctttttgca
10321 gcagcacctg ttatttacca cagctgtgta actgctgggt ttcagatctt gtccttcttc
10381 agacacagtg catatttctg actcttttgt actaacctta ttatttaagg tctaggtcat
10441 ctaggttctt ggaacagata ctaaataaac tcttgtaaga gcaaatacct ttgtatggtt
10501 cttccttcta actctaggta gatcttttta cctaattcta gccccatccg aagaaaatca
10561 ctgtcaggat cagagcttga tttcaaatag gagtttctgc taaagagttg ctggaaaaaa
10621 cacagttatc caaaggactc tgattcttct ctactatccc aggaagcaat gagggtcaac
10681 actcaggtga aggacttaac tatgttttct gttaatgact cagaagaaac ttgagctata
10741 caggggtgaa aacaaaatct ctgaattgat gcgtttgcct ctgcaccata caatgtaaat
10801 tcagagaagc tatgaatagg tctacgtagc tggaagtcat aaaaaggcaa ccactgttaa
10861 aaatatctgt aattctcagt taataacaac aaatgagaaa aggagtatta cttattccaa
10921 ctttgttact gttgcttgaa gttcatttta ttgttgtttt tagtgagatg atacatctac
10981 agtttcagga gtaatgccac tgaattc
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