GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS CHKMY05 4349 bp ds-DNA VRT 28-MAY-1991
DEFINITION Chicken gene for myosin light chain (exons 5-9)
ACCESSION X00460
KEYWORDS alternate splicing; myosin.
SOURCE Gallus gallus DNA.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 4349)
AUTHORS Nabeshima,Y., Fujii-Kuriyama,Y., Muramatsu,M. and Ogata,K.
TITLE Alternative transcription and two modes of splicing result in two
myosin light chains from one gene
JOURNAL Nature 308, 333-338 (1984)
STANDARD full automatic
COMMENT This represents the first report of a cellular gene which produces
two polypeptides from a single gene by differential initiation of
transcription followed by a different mode of splicing.(see x00458
and x00459) LC1 mRNA: exons 1,4,5,6,7,8,9 LC3 mRNA: exons 2,3,5,6,
7,8,9 also see <GGMY03> and <GGMY04>
From EMBL entry GGMY05; dated 06-JUL-1989.
FEATURES Location/Qualifiers
intron <1..283
/note="intron IV"
mRNA 284..427
/note="exon 5"
CDS 284..427
/note="myosin light chain part 5"
/codon_start=284
intron 428..1941
/note="intron V"
mRNA 1942..2115
/note="exon 6"
CDS 1942..2115
/note="myosin light chain part 6 (1942 is 2nd base in
codon) (2115 is 1st base in codon)"
/codon_start=1942
intron 2116..2582
/note="intron VI"
mRNA 2583..2660
/note="exon 7"
CDS 2583..2660
/note="myosin light chain part 7 (2583 is 2nd base in
codon) (2660 is 1st base in codon)"
/codon_start=2583
intron 2661..3163
/note="intron VII"
mRNA 3164..3189
/note="exon 8"
CDS 3164..3189
/note="myosin light chain part 8 (3164 is 2nd base in
codon)"
/codon_start=3164
intron 3216..4020
/note="intron VIII"
mRNA 4021..4349
/note="exon 9 (284 is 2nd base in codon) (427 is 1st base
in codon)"
misc_feature 4311..4316
/note="polyadenylation signal"
BASE COUNT 1323 a 963 c 939 g 1124 t
ORIGIN
1 cttattttac tctgtgcttg aaataactac aaggtcaatt gcaaaactac tggtggcttt
61 tagagtcaag aaagctacaa aaggtagtca taaaagttta aaaatagaga aacccaaaca
121 tcagagagga ggagtttctc ttggaactgc aggccaaaga ttcccctgct aactcccctg
181 gagtcgttgg aacgtacagg agacaacagg cccacagctt ggtcaatgca acgggtcaaa
241 gtgtagctcc ctgcctcaac taaccattgc tcttcccttg cagacttcaa ggaggccttc
301 ctcctctttg acaggactgg tgatgccaag attaccctga gccaggtcgg tgacatcgtt
361 cgggcactgg gacagaaccc cacaaacgct gagatcaaca agatcctggg caaccccagc
421 aaagagggta agaaccctgc tatctctctt ggaccttttt cagactgggg aactaattag
481 gaaacatcag aactcccagt aggaggaaga cgattttttg ctccaatagg gaagcaaggc
541 gggcactcca acagcagcaa ggggaatttt gccagtttct tttgaagaag attgggggca
601 gcagggaagt actgcgttct aggaaaagga agccttatct ctgggctatg gagtgtctct
661 gcagagagat cactttgtcg tcagcttgga gcgcacgcac ttcttactgc tcacagtctc
721 tttgtgaccc agactcctca gcaaggggct atttcaagca gcagttcttt tgcattagct
781 gagacatacc ccaagagtag aaacccaact gtatattgta gctgaacaga atggcctttt
841 taaaactgct acaaacctag tcacgcacag cacaatttat tactggtcat ttactaactg
901 tcactttcta tttcctttct tcctctgtag agataaaaag tggtaaattt tgtgataatc
961 tccttttaac aaggacttgg ctgcaggaca tcaccaattc cagagagtca catattagga
1021 taggccaaac aatatccaaa ccatccggct ggaaagagag gctctaagga acagtttgta
1081 agtcctgagt taagcattag catcagtggg aagtgctaag cagaagacta ataccgagct
1141 gagacagtgc tttaagtgac tataccaata actgaacgtc tttatatagc tgggtagtga
1201 aatagtttct gtaacaacta tcttttcaaa aagctcttat atttccacct attatatttc
1261 atcctcaccc agatgaaagc tgctccttga aagggtttaa gtaagaaaga aaaagaaagg
1321 gatccttgcc atctgggaag gcctcactga agaccttcag cttctggaaa caaaggggca
1381 agatgcaagc ttgtgaggcc ttaaacctcc gcagtgagca tgctgaatag cagatttgga
1441 taccacctcc actgagcgtc ctatttttag ttggtttatt cttttgccaa gtctctgcta
1501 tcacatccaa gagaaagtga tgaggccaat aacacaaagt tgacaaagcc agggggggct
1561 ttaagtcctg cactcacctc tgccttaaaa cctaggcagt agtaaaggag ttctgtttca
1621 gtgtaattct gtaagctccc ccattgccaa aatctttttg aagatcaact cgcgtagcac
1681 gcttcatttc tgaaaggaaa tatgcaacac atgcacttta taaagccatg ggaaaagtgg
1741 agtagagaaa ggatgtgaaa aaatcagatg gattccaaaa ctaactccaa caataggaga
1801 atctgattct gcagaacagc caaccccctg tggtgaaaga gccttgcaag tgaggtgtga
1861 ggcttcccca aatttcatgt gaatatccta ggtgaacatt caagtatcca gcagcattgc
1921 acatgatagc tttctttgca gaaatgaatg ccaagaagat cacttttgaa gagttcctgc
1981 ccatgctgca aatggcagct gctaacaaca aggatcaggg tacctttgaa gactttgttg
2041 aaggtctgcg tgttttcgac aaggaaggca acggcacagt cggggctgag ctccgccatg
2101 tactggctac actgggtaag ggattatcgc actgtgaaca caaacaattg ctgctgtttg
2161 aaggcatgag agagggagga agaatgttgt aactagctta gacaggtggg aggctgctgg
2221 ccaagactct tggaaagctg atgcaaagca acagaaagat caaaagaaag agttttactt
2281 ctgaactcaa attaggtact aaaagggtcc atgtagggag agagagaaaa ggctgtaatc
2341 tctagtggcc taaagcacac agatagagga tttgctgcat agttcttcac tctgtaaatg
2401 gctgggaccc tatgtctacg tgcatgtgca gaacttgtaa agaaagttaa aatgaataag
2461 tcttagttca gcaacagatc ttcttgctac tatctcagaa aacaaagaaa gcatttggaa
2521 aagctgcagg attcagcctc agctgtctgt ttaagaatta accaggctgc cttggcctcc
2581 aggcgagaag atgacagaag aggaagtaga agaactgatg aaaggtcagg aagactccaa
2641 cggctgcatc aactacgagg gtacgtgtgg gggttcaaca ccctcttgtc ctacatttca
2701 tccctgagtc acattgcagt tggctcttag ctatgctttg cccacccaat ttgccagaga
2761 agcttaagtt acgttgaaag gtttatgcat gaaaatgatg tgcctaccca gcagtcacac
2821 cacgggaaag gctacgttca gccacagcga gagaaggcca ctgctgttag ccctggtgct
2881 ctaggctctg acattctgta gaaaagctgc cataattgga agtaaactaa cccacagcta
2941 atgcttgggc aaaatgatcc atctgacatt cagtctcatc acatccaagc acgggacgag
3001 tattaaacac ttcaaagttt ctatcacttt tgctgagtca aatctgatca gaagcgtgcc
3061 ttttcaacac catcagctcc tgagcagctc ttgcttttga aggtgtctcc tgaagtaata
3121 tttgctgctc cttctaacta tcacatccgt attttttccg cagcatttgt aaagcacatc
3181 atgtctgtct aagaggacgt ccccagataa caaatggtac gttcccaatt tgcatgctta
3241 gcctcgtctc tttgctatgc ttataggctt cttcttccca tggccagaca taggttgtga
3301 aatcaacatg acactgtctc accaatgata aaattaaact gctgtccaag agcaaagagc
3361 acaggaaggc agatctctgg gtcttattcc cagctctaca actggttaga aactcagatg
3421 ccatttattg gatttttccc acagccaaat cgtgaaaaaa tgctcagttt acctatgagc
3481 atgaaaaaaa cttgagctta aatatcatct tagatttctg ttgtcatata agagttcaca
3541 ctatgcaatt gacaccttga gaatagatca gaaacaacta ataacactct gtatcagtgc
3601 gtgaagacgc tacacatgtg acacggacgc tttcaaagaa atgaagtaat tatctgaaga
3661 aagtatttta acagtcatgg tcaatagcca aattggtggg cttcagaagt gcattcagca
3721 gtatttctac aagcaggggg aaaagaaaag caataaaaga aacaacacaa cccacaacct
3781 tcatatgctt ctattttgtc agcagaaaag ctttcagtta cctctctttt aaaatgcaaa
3841 caagataccg ggggtgtgtg ggctcctttg agctgctatc ctatgctggg agagacaatc
3901 ccaactcacc gtgcaactaa acaggcatct caaaagcaaa attaagggct ccatttgtca
3961 ttttgcagtg tttcttcagc caaaggtgat ttcaaatcga actcttccat tccttcctag
4021 aacaagttga attcggaaag accagtaggc acctaagatc tgcctgagat gaaattcttg
4081 ctctggcagt catcatcaac atggaaactg cgtagactgg acaatatgcc tccaaaccca
4141 ctacttttct ttttaaccag tgcatccata ggcattgcct ctgggtcact tccaaaaact
4201 gaactttcac agggagagtt agatggaaaa actgccactg tccagcattc atccatccca
4261 agcatctctt tccatgttgt ttcccatgaa agaatgtaaa cctctggaaa aataaagcat
4321 cagtaaatcc ctgttgtcac tttgaggct
//GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS CHKMY04 1365 bp ds-DNA VRT 28-MAY-1991
DEFINITION Chicken gene for myosin light chain (exons 2-4)
ACCESSION X00459
KEYWORDS alternate splicing; myosin.
SOURCE Gallus gallus DNA.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 1365)
AUTHORS Nabeshima,Y., Fujii-Kuriyama,Y., Muramatsu,M. and Ogata,K.
TITLE Alternative transcription and two modes of splicing result in two
myosin light chains from one gene
JOURNAL Nature 308, 333-338 (1984)
STANDARD full automatic
COMMENT EPD; 07064; Gg myosin LC3.
This represents the first report of a cellular gene which produces
two polypeptides from a single gene by differential initiation of
transcription followed by a different mode of splicing.(see x00458)
LC1 mRNA: exons 1,4,5,6,7,8,9 LC3 mRNA: exons 2,3,5,6,7,8,9 also
see <GGMY03> and <GGMY05>
From EMBL entry GGMY04; dated 06-JUL-1989.
FEATURES Location/Qualifiers
intron <1..347
/note="intron 1"
promoter 318..325
/note="promoter structure"
mRNA 348..421
/note="exon 2"
CDS 419..421
/note="myosin light chain part 2"
/codon_start=419
intron 422..998
/note="intron 2"
mRNA 999..1023
/note="exon 3"
CDS 999..1023
/note="myosin light chain part 3"
/codon_start=999
intron 1024..1269
/note="intron 3"
mRNA 1270..1297
/note="exon 4"
CDS 1270..1297
/note="myosin light chain part 4 (1297 is 1st base in
codon)"
/codon_start=1270
intron 1298..>1365
/note="intron 4 (1023 is 1st base in codon)"
BASE COUNT 374 a 318 c 310 g 362 t 1 others
ORIGIN
1 acatggcacc tcattgccct cagcgctcct cgattgggga ctatagctta ttggagaaat
61 aggagcctct cctatcatgg taggcccgga caaggaatat cccctcttga tggtccagac
121 cccagaaagt gctgacatga tccaaacttg ctgcttgcaa ctcccttgcc cctctccaac
181 ctcctaaaaa cagcagccga aaggcccatc aaggtacggt gccacctgtc actttgccca
241 agaaagctag agaactcacc aaggggaggg gcagcttcct tctgacgcct aatccagcaa
301 tgccgtcgcg ctgccagata aataagggga agaaaggcca ggaaagcagg accactctca
361 gagacctctc agctaatcct ccggcccgtc cgccgctcta cttttccaac tctcaatcat
421 ggtgggttca ctgcagacta aacgttagca gcgaggcagt gtattttaca gcagtaaatt
481 cagcttcttg ttcaagttag tcctaggggg gaggataggc caagaggtga cagttcaggt
541 ttgcttgatt ttcctctgaa tgtttttaat acaagagagt atcatagctt aaygctttat
601 gaacaaaagc ttggaaggca tgatttattt tctatgttaa tgtttaaaaa gaaaaaaaaa
661 aagaaaaaac ccgccaggta ttaaatatta cctcaaatgg gttggtattt gagtctatac
721 taaagtatta agtgacctgg tggtacataa acaacctatt tgttaagaaa ctgtagatta
781 ttagtgcttc tggccacaac cttgctgttc tcagggatgc aggtttttac aagagcccta
841 aaaacaggcc tggggatgca tttttacatg gcaggttgcc agtttgggtt tctgtggcct
901 aattacatca ggcaaggcag atatgggaat agctcagcac ctgtgcattt tcagcagtac
961 tacttctcct ttttttcttt ctctcttccc aactgcagtc cttctcacct gacgaaatca
1021 atggtaagtg aaacaaaaac taaactaaca gggggcagga ggggggaccg taacggctac
1081 caaaaagcta aacgttcacc ggtcttaaga gctcttttgc catctgcatc acttctgtgc
1141 atctgtcagg ccacggtttt tgctgtcttg gaagatactc atggattgag ctggatgctt
1201 tagaaaacaa gcaggttctc ccccagcccc cccttcctaa gctatttttt gtgattgatt
1261 tccgaccaga tcgagttctc caaggagcag caggatggta agttacaaga cagctgatgc
1321 gtttgacatg tatgtgagtt cagaggggag ctacagtctt gaaag
//