GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS CHKPPG 1576 bp ss-mRNA VRT 28-MAY-1991
DEFINITION Chicken mRNA for prepro-glucagon
ACCESSION Y07539
KEYWORDS glucagon; hormone; preproglucagon.
SOURCE Gallus gallus RNA.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 1576)
AUTHORS Okamoto,H.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1576)
AUTHORS Hasegawa,S., Terazono,K., Nata,K., Takada,T., Yamamoto,H. and
Okamoto,H.
TITLE Nucleotide sequence determination of chicken gluagon precursor
cDNA. Chicken preproglucagon does not contain glucagon-like peptide
II.
JOURNAL FEBS Lett. 264, 117-120 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P01277; GLUC$CHICK.
Data kindly reviewed (03-JUL-1990) by Okamoto H.
*source: developmental stage=6 week old male; tissue=pancreas;
*source:library=lambda ZAPII;
From EMBL entry GGPPG; dated 26-SEP-1990.
FEATURES Location/Qualifiers
CDS 89..541
/note="prepro-glucagon (AA -22 to 129)"
/codon_start=89
CDS 89..154
/note="signal peptide (AA -22 to -1)"
/codon_start=89
CDS 155..541
/note="proglucagon (AA 1 to 129)"
/codon_start=155
CDS 251..337
/note="glucagon (AA 33 to 62)"
/codon_start=251
CDS 344..415
/note="intervening peptide (AA 65 to 87)"
/codon_start=344
CDS 422..532
/note="glucagon-like peptide (AA 90 to 126)"
/codon_start=422
misc_feature 1544..1549
/note="polyA signal"
misc_feature 1560..1565
/note="polyA signal"
BASE COUNT 482 a 335 c 238 g 521 t
ORIGIN
1 aggatcctca aggctttcca tagttccaga agagagagta gcagatcttt ctccaactgc
61 tacatatcac ttcaaaatat aaaaggcaat gaaaatgaaa agtatttatt ttattgctgg
121 actcctttta atgatagttc aaggcagctg gcaaaatcct cttcaggata cagaggagaa
181 atcaagatca ttcaaagctt cccagtctga accattagat gaatctaggc agctgaatga
241 agtaaagcgt cattcacaag gcacattcac cagtgactac agcaagtact tggacagcag
301 acgagctcag gattttgtgc aatggttaat gagcactaaa agaaatggcc aacaaggaca
361 ggaggacaaa gagaatgaca aattcccgga tcagctctca agtaatgcaa tttccaagcg
421 tcattctgaa tttgagagac atgctgaagg cacctacacc agtgatatca cctcttattt
481 ggaaggtcaa gctgccaaag aattcattgc ttggttagtg aatggacgag gaagaagaga
541 gtaagaatcc atccattcct attattaaat tttgctttgc tttcaaggtt agaaatcaaa
601 tctgatgaaa tcttgatgta tttttgatca ttcctactgt acttcacgtt ttccttttgt
661 aatagaagat actgtgtggc tgttcttaag ttcaaacatc ttaagttatt caaacatctt
721 aagttattaa gttcaaacat agactgaaac aaacatgttc agtatactgt tacactctta
781 aaggtcaaat tatatttaag gtcaatatat ctaaaactaa accagagttt cctctaatta
841 acaccttttc ctcatcacat aacattgctt acagtctcct taactctcac agagatgagt
901 catgttagtg tcgtcttaaa tttccctttt tctctgcctc tgtagtcaat catttcctct
961 gcaatatttc catgatgaaa tttttaacaa tgtcactgcc accacagcca ttttccatgc
1021 tctggtgatc attcccagtt actgatgtct tcttttgctc ttcctgttat ccacaaagtc
1081 tccacaaatt gtagcatcaa aaataaccat aatttcctac agctttccct tattttcctt
1141 tttaaagcac ctctacttct tttgctatag ttcacagttt ccaagactca gctccatagt
1201 tttgattcag gtacatctca gactttttca ttatcttgct ctccatatat tcagtgcaaa
1261 tttctcaaac ctattcactt catctgctca cccatgattt tgtatccttt tcatgagact
1321 tactcttgga acagccttaa tttcaggcac tttctgtctt atcaccattc caagtctgtg
1381 cagttaggag ttacatcaca cacacaccac tctctacctt ttaactgttc tgtacttttt
1441 gtgcctttag actgtaagct cttcggggca aggatttcag ttcatcctat tattatatgt
1501 tttgctagct gtaaagtact atgtaaatct ctcatataaa aataataaat aacaataaca
1561 ataaaatata atctat
//