GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS CHKRMLCK 1824 bp ss-mRNA VRT 28-MAY-1991
DEFINITION Chicken rMLCK mRNA for recombinant myosin light chain kinase
ACCESSION X52877
KEYWORDS kinase; myosin light chain kinase.
SOURCE Gallus gallus RNA.
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 1 to 1824; 1 to 1824)
AUTHORS Watterson,D.M.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: developmental stage=embryo; Recombinant MLCK was assembled
from three overlapping cDNA clones with synthetic oligolinkers
added at both the 5' and 3' ends to provide sites for ligation into
pVUCH1 bacterial expression vector.
From EMBL entry GGRMLCK; dated 31-JUL-1990.
FEATURES Location/Qualifiers
misc_feature 1..18
/note="synthetic 5' linker cassettte"
CDS 6..1814
/note="myosin light chain kinase (AA 1-603)"
/codon_start=6
misc_feature 1813..1824
/note="synthetic 3' linker cassettte"
BASE COUNT 604 a 368 c 442 g 410 t
ORIGIN
1 aatttatggc gaattccggt gcttgcaaag tgcttgtgga agatacctca tccacaaagg
61 ctgcgaagcc agctgagaaa aagaccaaga agcctaagac cacacttccc cctgtgctgt
121 caacagaaag cagtgaagca acagtgaaga agaaaccagc tccaaagact cctccaaaag
181 cagctactcc ccctcagatc actcagttcc cagaagacag aaaagtccgt gctggtgaat
241 cagtggaatt gtttgccaaa gtggtaggaa cagcacccat aacttgcacc tggatgaaat
301 tccgtaagca gattcaagaa aatgaatata tcaaaattga gaatgctgaa aacagcagca
361 agctaacaat atcatcgaca aagcaggaac actgcggatg ttacacgcta gttgtagaaa
421 acaaacttgg cagcagacaa gcacaagtca accttacagt tgttgataaa ccagacccac
481 ctgctggaac gccttgtgca tcagacatac ggagttcttc cctcactctt tcatggtacg
541 gctcctctta tgatggtgga agtgcggtgc agtcctacac tgtggagatc tggaactcag
601 tggataacaa atggacagac ctcactacct gccgcagcac gtctttcaac gtgcaggacc
661 tgcaggctga ccgggagtac aaattccgtg tacgagctgc taacgtgtac ggcatcagtg
721 agcccagtca agaatctgag gtggtaaaag ttggagagaa acaagaagaa gaacttaaag
781 aggaggaagc agagctgtct gacgatgaag gcaaagaaac agaggtcaac tatcggacag
841 ttactatcaa caccgaacag aaagtttcag atgtctataa tattgaagaa agactgggat
901 cggggaaatt tggacaagtc tttaggcttg ttgaaaagaa gactggaaaa gtgtgggcag
961 gaaaattttt caaagcatat tctgcaaagg aaaaagagaa cataagggat gaaatcagca
1021 tcatgaactg tctgcatcac ccaaagctcg tgcaatgtgt agatgccttt gaagaaaaag
1081 ccaacattgt tatggtcttg gaaatggttt ctggaggaga actctttgaa cgtatcattg
1141 atgaagactt tgaactgaca gagagagaat gcattaaata tatgaggcag atttcagaag
1201 gagttgagta catccataag cagggtattg tccatttgga tttgaagcca gaaaatatta
1261 tgtgtgtcaa caagactggt acaagtatca aactcattga cttcggactt gcaagaagat
1321 tagaaagtgc aggttctctc aaagttctct ttgggacacc tgagtttgtg gctccagaag
1381 ttataaacta tgaacctatc ggatatgaaa cagatatgtg gagtatagga gttatctgct
1441 acatcctggt cagtgggctt tctcccttca tgggagacaa cgacaacgag accctggcca
1501 atgtcacctc tgccacgtgg gacttcgatg atgaggcttt tgatgaaatc tctgatgatg
1561 ccaaggactt tatcagcaat ctcctgaaga aagacatgaa gagtcgctta aattgtactc
1621 aatgtcttca gcacccgtgg ctgcaaaaag acaccaaaaa catggaagcc aaaaaacttt
1681 ccaaagatag gatgaagaaa tatatggcca gaagaaaatg gcagaaaaca ggccatgctg
1741 tccgagcaat aggaagactg tcatccatgg caatgatttc tggtatgagt gggaggaagg
1801 cctctgggag ctcgtagctg cagg
//