GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS XELK81B2G 751 bp ds-DNA VRT 28-MAY-1991 DEFINITION Xenopus laevis XK81B2 keratin gene exon 1 and 5' flank ACCESSION X12730 X05180 KEYWORDS keratin. SOURCE Xenopus laevis DNA. ORGANISM Xenopus laevis Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Amphibia; Lissamphibia; Anura; Archeobatrachia; Pipoidea; Pipidae. REFERENCE 1 (bases 1 to 751) AUTHORS Miyatani,S., Winkles,J.A., Sargent,T.D. and Dawid,I.B. TITLE Stage-specific Keratins in Xenopus laevis Embryos and Tadpoles: The XK81 Gene Family JOURNAL J. Cell Biol. 103, 1957-1965 (1986) STANDARD full automatic COMMENT SWISS-PROT; P05781; K1C4$XENLA. *source: clone=G8103; Three homologous regions upstream of the TATA-box were observed in genes A1, B1 and B2. It may be speculated that these regions contain signals for the temporally and regionally controlled activation of the keratin genes during frog embryogenesis. See X05865 for corresponding cDNA sequence (clone DG118). The genomic clone G8103 was sequenced up to the end of exon 4. In the sequence overlap of 776 nucleotides available between cDNA DG118 and genomic B2 exons on phage G8103 there were no differences. See also X04668 for gene A1 and X05154 for gene B1 (partial). From EMBL entry XLK81B2G; dated 06-JUL-1989. FEATURES Location/Qualifiers misc_feature 13..30 /note="homologous to A and B genes (see CC)" misc_feature 58..93 /note="homologous to A and B genes (see CC)" misc_feature 165..173 /note="homologous to A and B genes (see CC)" promoter 236..242 /note="pot. TATA-box" mRNA 257..751 /note="pot. exon 1" precursor_RNA 257..>751 /note="pot. transcript (by A1 and B1 homology)" CDS 314..751 /note="B2 keratin (AA 1-146)" /codon_start=314 BASE COUNT 187 a 152 c 206 g 206 t ORIGIN 1 ttcagcaaag ttgcctgggg caaggtgaga gggacggctg aaaactggaa agaaaagaag 61 aatttgcctc tcccagtttc taaccaatac cttcagggct atatctttta tgtaaatatg 121 gactgattat gatggtgatt ttctggaaac agatgctggc ctggcaaaaa aagtatttac 181 tctgggaggc tgtccctggt gtaactgaaa cacccaccac tctttgtgta tccactatat 241 atataggctg tcaaagctgc aggcacccat tctgttttgt gcaggctaag ttgtcaagca 301 atacacagcc accatgtcat tccgttccag ctcttcttac tctctacaga gcaaaggcat 361 ctctggaggt ggtggctatg gagcaggctt tggtggtggc tctggagcag gctttggtgg 421 tggctctgga gcaggctttg gtggtggcta tggagcaggc tttggtggtg gtgcatcttc 481 tgggttttcc cttagttctg caggtggttt tggagcagct gctgccagtt ccagcttcag 541 caactttggt ggtaatgata agcaaaccat gcagaacctc aatgaccgtc ttgcttccta 601 cctggagaaa gtaagagccc tggaagcagc caatgctgac ctggaactga agatccgtga 661 gtggtatgag aagcaaaaag ggtctggtat tggagctggg tctaaggatt tcagcaaata 721 ctttgaaatc atcagtgatt tgaggaacaa g //