GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS XELK81B2G 751 bp ds-DNA VRT 28-MAY-1991
DEFINITION Xenopus laevis XK81B2 keratin gene exon 1 and 5' flank
ACCESSION X12730 X05180
KEYWORDS keratin.
SOURCE Xenopus laevis DNA.
ORGANISM Xenopus laevis
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Amphibia;
Lissamphibia; Anura; Archeobatrachia; Pipoidea; Pipidae.
REFERENCE 1 (bases 1 to 751)
AUTHORS Miyatani,S., Winkles,J.A., Sargent,T.D. and Dawid,I.B.
TITLE Stage-specific Keratins in Xenopus laevis Embryos and Tadpoles: The
XK81 Gene Family
JOURNAL J. Cell Biol. 103, 1957-1965 (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P05781; K1C4$XENLA.
*source: clone=G8103; Three homologous regions upstream of the
TATA-box were observed in genes A1, B1 and B2. It may be speculated
that these regions contain signals for the temporally and
regionally controlled activation of the keratin genes during frog
embryogenesis. See X05865 for corresponding cDNA sequence (clone
DG118). The genomic clone G8103 was sequenced up to the end of exon
4. In the sequence overlap of 776 nucleotides available between
cDNA DG118 and genomic B2 exons on phage G8103 there were no
differences. See also X04668 for gene A1 and X05154 for gene B1
(partial).
From EMBL entry XLK81B2G; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 13..30
/note="homologous to A and B genes (see CC)"
misc_feature 58..93
/note="homologous to A and B genes (see CC)"
misc_feature 165..173
/note="homologous to A and B genes (see CC)"
promoter 236..242
/note="pot. TATA-box"
mRNA 257..751
/note="pot. exon 1"
precursor_RNA 257..>751
/note="pot. transcript (by A1 and B1 homology)"
CDS 314..751
/note="B2 keratin (AA 1-146)"
/codon_start=314
BASE COUNT 187 a 152 c 206 g 206 t
ORIGIN
1 ttcagcaaag ttgcctgggg caaggtgaga gggacggctg aaaactggaa agaaaagaag
61 aatttgcctc tcccagtttc taaccaatac cttcagggct atatctttta tgtaaatatg
121 gactgattat gatggtgatt ttctggaaac agatgctggc ctggcaaaaa aagtatttac
181 tctgggaggc tgtccctggt gtaactgaaa cacccaccac tctttgtgta tccactatat
241 atataggctg tcaaagctgc aggcacccat tctgttttgt gcaggctaag ttgtcaagca
301 atacacagcc accatgtcat tccgttccag ctcttcttac tctctacaga gcaaaggcat
361 ctctggaggt ggtggctatg gagcaggctt tggtggtggc tctggagcag gctttggtgg
421 tggctctgga gcaggctttg gtggtggcta tggagcaggc tttggtggtg gtgcatcttc
481 tgggttttcc cttagttctg caggtggttt tggagcagct gctgccagtt ccagcttcag
541 caactttggt ggtaatgata agcaaaccat gcagaacctc aatgaccgtc ttgcttccta
601 cctggagaaa gtaagagccc tggaagcagc caatgctgac ctggaactga agatccgtga
661 gtggtatgag aagcaaaaag ggtctggtat tggagctggg tctaaggatt tcagcaaata
721 ctttgaaatc atcagtgatt tgaggaacaa g
//