GenBank-Updates@genbank.bio.net (01/19/91)
LOCUS HUMALP1GC 2487 bp ss-mRNA PRI 19-JAN-1991
DEFINITION Human alkaline phosphatase (ALP-1) mRNA, complete cds.
ACCESSION J04948
KEYWORDS alkaline phosphatase.
SOURCE Human choriocarcinoma cell line JEG 3, cDNA to mRNA, clone TPAP15A.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 2487)
AUTHORS Watanabe,S., Watanabe,T., Li,W.L., Soong,B.-. and Chou,J.Y.
TITLE Expression of the germ cell alkaline phosphatase gene in human
choriocarcinoma cells
JOURNAL J. Biol. Chem. 264, 12611-12619 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Biol. Chem. 264,
12611-12619 (1989)] kindly submitted
by J.Y.Chou,03-JUN-1989.
FEATURES Location/Qualifiers
mRNA 1..2487
/note="ALP-1"
CDS 53..1651
/note="alkaline phosphatase precursor (EC 3.1.31.)
/nomgen='ALPP' /map='2q37' /hgml_locus_uid='LV0092C'"
sig_peptide 53..142
/note="alkaline phosphatase signal peptide"
mat_peptide 143..1648
/note="alkaline phosphatase"
BASE COUNT 487 a 827 c 724 g 449 t
ORIGIN 189 bp upstream of PstI site; chromosome 2q37.
1 catactccat acctgggatt tccgcctcgc cgctctccga ctgcttccag acatgcaggg
61 gccctgggtg ctgctcctgc tgggcctgag gctacagctc tccctgggca tcatcccagt
121 tgaggaggag aacccggact tctggaaccg ccaggcagcc gaggccctgg gtgccgccaa
181 gaagctgcag cctgcacaga cagccgccaa gaacctcatc atcttcctgg gtgacgggat
241 gggggtgtct acggtgacag ctgccaggat cctaaaaggg cagaagaagg acaaactggg
301 gcctgagacc ttcctggcca tggaccgctt cccgtacgtg gctctgtcca agacatacag
361 tgtagacaag catgtgccag acagtggagc cacagccacg gcctacctgt gcggggtcaa
421 gggcaacttc cagaccattg gcttgagtgc agccgcccgc tttaaccagt gcaacacgac
481 acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag aaagcaggaa agtcagtggg
541 agtggtaacc accacacggg tgcagcatgc ctcgccagcc ggcacctacg cccacacggt
601 gaaccgcaac tggtactcgg atgccgacgt gcctgcctcg gcccgccagg aggggtgcca
661 ggacatcgcc acgcagctca tctccaacat ggacattgat gtgatcctag gtggaggccg
721 aaagtacatg tttcccatgg ggaccccaga ccctgagtac ccagatgact acagccaagg
781 tgggaccagg ctggacggga agaatctggt gcaggaatgg ctggcgaagc accagggtgc
841 ccggtacgtg tggaaccgca ctgagctcct gcaggcttcc ctggacccgt ctgtgaccca
901 tctcatgggt ctctttgagc ctggagacat gaaatacgag atccaccgag actccacact
961 ggacccctcc ctgatggaga tgacagaggc tgccctgctc ctgctgagca ggaacccccg
1021 cggcttcttc ctcttcgtgg agggtggtcg catcgaccat ggtcatcatg aaagcagggc
1081 ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc attgagaggg cgggccagct
1141 caccagcgag gaggacacgc tgagcctcgt cactgccgac cactcccacc tcttctcctt
1201 cggaggctac cccctgcgag ggagctccat cttcgggctg gcccctggca aggcccggga
1261 caggaaggcc tacacggtcc tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg
1321 cgcccggccg gatgttacgg agagcgagag cgggagcccc gagtatcggc agcagtcagc
1381 agtgcccctg gacggagaga cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc
1441 gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc atagcgcacg tcatggcctt
1501 cgccgcctgc ctggagccct acaccgcctg cgacctggcg ccccgcgccg gcaccaccga
1561 cgccgcgcac ccggggccgt ccgtggtccc cgcgttgctt cctctgctgg cagggacctt
1621 gctgctgctg gggacggcca ctgctccctg agtgtcccgt ccctgggggc tcctgcttcc
1681 ccatcccgga gttcccctgc tccccacctc cagtcgtcct gccggacctc cacctggagc
1741 tgtcaccccc ggagtcgcca cacagacgtt cctgccatgg aaccttccct cccggtgcac
1801 cctggggacc gagcccttga caccacgcct ttgctttatc ttgctcttaa attttggccc
1861 caactccagg gactggggat ttgtgcctgg cagctgcctg catttcagga aaagaggagg
1921 ctcagaccat ccagcccccg cccatatcct gaggtggatc aggcaggctc tctccccggg
1981 gacatgaggc acccatacct aggaccccct gcgccttttt tagcttcagt catggcagca
2041 cctgagggac acaaggactt gggtgcatca ggacgccttg gagaagcgtg gcttcctgcc
2101 accctgcaac ccaccctccc agccaaggag gctgctgtgg tggggatccc caggggcttt
2161 gacacagtcc tctgctgtcc tccactgggc taattctaca cccctgtgcc cctcctaggg
2221 gcccatgagt cagagaggct tgccccaagt cacagccact cagatgttcg acgcccccta
2281 aggtccattc cagcacccac ctgagttccg aggagcacct gggaagctct gggtgcagga
2341 tagcagtcca gagtccatgc cccgctaggc catctgggtg ctgggcatgg atttctcagc
2401 aaggaagact cattaccttc cctccctggg cctccattct ctcctgggaa acacaaagca
2461 ataataaaag gaagtgttag acaatgt
//GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS HUMALPL 3101 bp ds-DNA PRI 29-MAY-1991
DEFINITION Human alkaline phosphatase (EC 3.1.3.1) gene.
ACCESSION D90054
KEYWORDS alkaline phosphatase.
SOURCE Human liver DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 3101)
AUTHORS Matsuura,S., Kishi,F. and Kajii,T.
TITLE Characterization of a 5'-flanking region of the human liver/bone/
kidney alkaline phosphatase gene: Two kinds of mRNA from a single
gene
JOURNAL Biochem. Biophys. Res. Commun. 168, 993-1000 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by: Fumio
Kishi Department of Pediatrics Yamaguchi University School of
Medicine Ube, Yamaguchi 755 Japan Phone: 0836-22-2258 Fax:
0836-22-2696
FEATURES Location/Qualifiers
repeat_region 375..664
/note="Alu sequence"
prim_transcript 2130..3101
/note="alkaline phosphatase mRNA and intron"
intron 2341..3101
/note="alkaline phosphatase intron"
repeat_region 2631..2926
/note="Alu sequence"
misc_feature 3051..3097
/note="putative VECTOR sequence Bacteriphage M13mp18
(M11454)"
BASE COUNT 775 a 752 c 692 g 882 t
ORIGIN chromosome 1; map position p34-36.1.
1 aagctttctc cagcgagtat gatggtttct gcaggttctt ggcataaagc ctttatcaga
61 ttaaggaaat tcttttcaat acctggtttg ctgagggctt ctgtcacatc gttttctgtg
121 accccattcc ctctccctag gtgagcacgt caagtttgat cagggtgtta aactgccacc
181 cctgtgccta tgattcccaa atttatactc taacccagac ttctttttca aatgccagag
241 ccaaatattc agctgcctcc ttagtgtctc cacttctaaa agacatctcc aactcaacat
301 atccaaaaac aagttcctga ttgtctccac ctcatgcctc aaaagaccac cccaaacgcc
361 gaaaggctga atgctttttt ctttttcttt tttttttttt tctgagatgg agtctcactc
421 tgttgcccag gctggactgc agtgatgcga tctcagctca ctgcaaactc tgcttcctgg
481 gttcaagtga ttctcctacc tcagcctctc aggtagctgg gactacaggt gcacaccacc
541 atgcccagct aatttttgta gagagagttt caccatgttg gccaggctgg tctcaaacac
601 ctgaccttaa gggatccacc cgcctcagcc tctcaaagtg ctgggattac aggtgtgagc
661 catcgcactt ggctcggtag tatatggctc agaaacattg ccatttacaa tagttcccca
721 aaaagcaaaa ttcttaggta taaatctgga ttcagagtcc agaatgctaa ccattacacg
781 atggaacccg taggtataaa tctaagaaaa catatccaag atctacaggc tgaagactac
841 agagtgctga taaaaccgaa gaactctgac tgaatgagtg gagagacgtg gtgtcttcat
901 gactgggcaa ctccatgtgg tatagacgta aaccctccca cattgatctg tggatttaat
961 accataccta tcaaaaacac agtggtggag gacagatcag ggatcgccag gtttagggat
1021 ggggggattg tgtaactata aagaacgcaa gagagatttt tggggtggca gagctgttct
1081 gggtcctgac ggtggcggtg gtggttacat aaatctatcc atgtgtcaaa cgtcagaaca
1141 ctcattttac acttgggggc aacagaaatc cctccctctg gagggggtga ctgatggtaa
1201 cctgattgct aattctggaa tcaggagccc tgtggtcagg tttctgctct gcaacttcct
1261 gttggtaacc ttgggcaagt ctccgtccag agccttggtt ttctcatctg taaaaggaga
1321 tgataggtcc ttttctgtcc actgcatagc tgattagtga aacatcatgg tgaaattctt
1381 tatgaactat ggagtgcagc acatagactt gctttcattt tgtcagtatc ctttatagat
1441 tgttcatgta agctcccaaa gagtagtatt tattttattg aaataaaatg cacgtagaga
1501 aaaatgtgtg tatcatacat tgacagctga acccaccgtg taaccagcac ccacccaccc
1561 agatcaatca taaaccgaac cgcaccagca ccccagcagc ccgttcccgt ttccgtaccc
1621 tccacgtgga gcctccgttc tgtctcccaa cgccctgggt tagtttttat actttctgtc
1681 atcggaatca cactgtaagt gctcttgggt ttagcttcct ttgctcaagc ttaccttgtg
1741 cgattcattc atgttgttgt gaggagctgt ggatcatcca ttctccttgc tgtctgtggt
1801 ggtttctgtg ttgtgaacac acacaatgta ttatccagcc tgccgtagat ggaggcagtt
1861 ttgaagccat tataaacagg gctgatgtgc acattctgct ggagagaaac gggtcccagg
1921 gtacaggtag gatgatcagc ttcggtagat cctgccggtt ttcccatgcg ctgtgcctgt
1981 ctgcactcca ccaacggcga gcggaccttc cggtagttaa acatcttcac gaactcttgg
2041 actttcctgc acacacagag aagataattt tggatggctc ttcccttccc cccacaacct
2101 tccttagggc actggctttc aactgatgta aatatttact atgccaagca ctaggagggc
2161 agagacaaac aagacaaagt cctcacactt agaaactccc ggtgtggcag ctgagatggc
2221 ccaggaaaga actatattac cttcaaaaag agaggtacat gcgatgtttg aggtggcatg
2281 aagctcagtg gtgttatatt ggaatgagtg agtgaccatc ctggagcctt cctgaaagag
2341 gtgacttcat ttttaagtga ttttaaataa tagtttaatg aattagtatt tcgtattcag
2401 ttaataacat ttttctgatt ttaggatttg ctatagaaat atttggaaac cgtaaagtag
2461 aacaaaaaaa aaatgtagga atcatctgaa attccaaatt ctaccactca cagttaagtg
2521 ttgttagatg ttagatgtgg gatattgcct tttaatttcc actctgcgcc gctaccccca
2581 gcccctaccc cagagccgtc acttctggca ctggagcgca gcttgcgtgg tttttttttt
2641 tttttttttt tttttgagac agagtcctgc ctgtcgccca ggctggagtg cagtggcgcg
2701 atctcggctc actgcaactc cccctcccgg gttcacgcca ttctcctgcc tcagcctcct
2761 gagtagctgg gactacaggc gcccgctacc tctcccggct aattttttgt atttttagta
2821 gagacggggt ttcactgtgt tagccaggat ggtctcgatc tcctgacttc gtgatccgcc
2881 cgcctcggcc tcccaaagtg ggcagatcac ctgaggtaga gagttcgaga ccagacctga
2941 ccaacatgga ccccatctct actaaaaata caaaattggc cagggcatgg tggcgcaagc
3001 tgctaatccc agccactcag ggaggctgag gctggaaaat tgcttgaacc cgacctgcag
3061 gcatgcaagc ttggcgtaat catggtcata gctgttttcc t
//