GenBank-Updates@genbank.bio.net (05/29/91)
LOCUS STMBAHBRP 5000 bp ds-DNA BCT 29-MAY-1991
DEFINITION Streptomyces hygroscopicus acetyl-hydrolase (bah), thioesterase
(ORF1 and 2), transport protein (ORF3), and regulatory protein
(brpA) genes, complete cds.
ACCESSION M64783
KEYWORDS N-acetyl hydrolyase; bialaphos biosynthetic gene cluster;
regulatory gene; thioesterase; transport protein.
SOURCE Streptomyces hygroscopicus DNA.
ORGANISM Streptomyces hygroscopicus
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 5000)
AUTHORS Raibaud,A., Zalacain,M., Holt,T.G., Tizard,R. and Thompson,C.J.
TITLE Nucleotide sequence analysis reveals linked N-acetyl hydrolyase,
thioesterase, transport, and regulatory genes encoded by the
bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus
JOURNAL J. Bacteriol. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 90..920
/product="acetyl-hydrolase"
/gene="bah"
/codon_start=90
CDS 917..1678
/note="ORF1"
/product="thioesterase"
/codon_start=917
CDS 1675..2454
/note="ORF2"
/product="thioesterase"
/codon_start=1675
CDS 2451..3794
/note="ORF3"
/product="transport protein"
/codon_start=2451
CDS 4098..4868
/product="regulatory protein"
/gene="brpA"
/codon_start=4098
BASE COUNT 689 a 1761 c 1719 g 831 t
ORIGIN
1 gagatctgaa cggagtgcgc gtggcatcgc ccgagttgga gctggtacgg gaactcatcg
61 aactcaactg gcatacccgc aatggtgagg tggaaccgcg gcggatcgcg tacgaccgtg
121 cccaggaggc cttcggcaac ctcggcgtcc cccctggcga cgtcgtcacc gtgggccatt
181 gcaccgcgga gtgggtacgg cccgcacggc aggacggcag gactctgctg tacctgcacg
241 gcgggtcgta cgcgctgggc tcaccgcagt cgcaccggca cctctccagc gccctcggtg
301 acgcggccgg agccgcggtg ctcgccctgc actaccgcag accgcccgag tcacccttcc
361 cggcagcggt cgaggacgcc gtggccgcct accgcatgct gctggaacag ggctgcccgc
421 cgggccgggt cacgcttgcc ggagactcgg ccggcgccgg gctcgccgtc gctgccctgc
481 aggccctgcg ggacgccggg accccactgc cggccgccgc ggtgtgcatc tcgccctggg
541 ccgacctggc ctgcgagggg gcgtcgcaca ccacccgcaa ggcgcgggag atcctgctgg
601 acacagcgga cctgcgccgt atggcggagc gttacctggc cggcaccgac ccccggcacc
661 cgctcgcctc acccgcccat ggcgacctga ccggtctgcc gcccctcctc atccaggtcg
721 ggtccgagga ggtccttcat gacgacgcgc gggcgctgga gcaggccgcg ctgaaggccg
781 gcacacccgt caccttcgag gagtggccgg agatgttcca tgtctggcac tggtaccacc
841 cggtgctgcc cgaggggcgc cgggccatcg aggtggccgg agcgtttctg cgcacggcca
901 cgggggaggg cctgaagtga ccgactggat ccagagcgtc tccgcgcccg acgccgtggc
961 ccgggtggtg tgcctgtccc gggcgggcgg cagcgcacgc gacttcgacc ggtggcgcgc
1021 acccatgggt gaggacgtgg agttggcggc ggtccagctg cccggacggc tcgaccgttt
1081 ccacgagcca ccgttgagcg atctgcacga gatcgccgaa gaggtggccg ccgccctgac
1141 caccctgccg gcccgcccgt acgtgctctt cggtgactgc atgggcgccc tgctcgcgtt
1201 cgagacggcc tgcgccctgc gccgccgggg cgccgccccg cccgactgcc tggtcgtcgc
1261 ctcctacccg gcacccgacc ggctgcgcac ggagcgcccc tacggcgacg gcagcgccga
1321 tgacctgcgg cagcggttgc gcgaggtcgg tggcgtcccg cccgccgtgc tcgacgagga
1381 cgagttgttc gagctcatgc tgcccatgct gcgcgcggac ttcgccgcgt tcgagggcta
1441 ccgccaccgg ccgaccgaac ccctctccgt ggacatccat gcactggtcg gcgccgacga
1501 cccttatgtg acggtcacgg acctgcacgg ctggcagcgc cacacgaccg gggagttcac
1561 cgcgcgggcg ctgcccggcg ggcacttctt cctgcacgag agtgacgacg cggtctcccg
1621 cgtccgctcc ctggcgctgg ccggcgcgag ggcggcacgg ccggggaggc ccgagtgacg
1681 ggagagcagg ggccggccga cccggcccag tggctgcggg cccatcgcac cgtgcccgat
1741 cctcaggtcc ggctgctgtg ctttccgcac gccggcgccg ggcccggcgt ctaccggccg
1801 tgggccgagg acatgccgtc gtacgccgag ctgtgggccg ctcgctaccc cggacgcgaa
1861 gaccggctgg cgcatccctt cgccaccagc ctgagcgaac tggccgacga cctcgcggcg
1921 gctgccgccg cggggctcag ccgggacgtc cccctggcgc tcttcggcca cagcatgggc
1981 gcggtcatcg cgcacgaggt cgccgtacgg ctggagaaca accacgattt ccggccggtt
2041 ctgctcgccg tctccgcccg ggagggcccg ctccaggtcg cccacgccgg actgcacctg
2101 ctcgacgacg acgccctggt cgcccgtacc cggggcatgg gcatccagcc gaacgccgac
2161 gcctacgccg acccggagct gcgcgagctg ttgctgcccg tgctgcgcga cgactgccgt
2221 ctgctggaga cccacccgac ttcccccgtc acacgggtgc gggcacccgt gctcgccttc
2281 gccgggcggt ccgatccggc gagcccggcg gccctgatga gttcctgggc cgaggccacc
2341 agtggcgcat tccgcctgct ggagatgcca ggggatcact tctacctgtt ctcgcacacc
2401 aaggacctgc tgtccgagct gatacccgct ctgtcccggg aggcccgtac atgaccgtga
2461 gggagagcga ccggacgacc gttccgcggt cccggtccct tcgccagctg gtgtgcggcg
2521 gcataggcca caccgtcgag tcgcatgact ggtacgtcta caccttcctc gccgtctact
2581 tctcggacga catcttcccc gagtcgtccg gcgacccctt ggtgccgctg ctcaacacct
2641 tcgccgtctt cgcgctggcc ttcgccgccc gcccggtcgg cgccacggtg atgggctggt
2701 acgccgaccg ctacggccgc cgcagcgccc tgatcgtgac catcctgctc atgggcctcg
2761 gcagcctgat gatcgggctc accccgagct acgcgacggc ggggccggtc gccccggtgg
2821 tgctgatagc ggcccgtctg gtccagggct tctcgctcgg cggcgaatac ggggccgcga
2881 ccaccttcct cgtcgagtcc gcggcccccg gccgccgggc gctgttcagc agcttccagt
2941 acgtggcgtc gtcggtcggc cacatcttgg cgggactgtc cacgctggcc gcctcccaga
3001 tctccgggga cggtatggac cgttggggat ggcggctgcc gttcatctgg ggcgcggtga
3061 tctgcctggc cgggctcgcc ctccggagca cggcagagga gaccttgccc accgggacgg
3121 aaggtggccg aaagaagacc cgcacgggcg cgttcgcggc gctgcgctcc cacccgaggc
3181 agacgttgct ggttgtcggc ctcaccatcg gcggcaatgt cgccttctac acgtggacca
3241 cctacttgcc cacctacgcc acggtgagca cgggcgcgga caaggacagc gccgtactgg
3301 cgggcaccgt ctcgctcatc ttcttcggct tgatccagcc actcggcggc ctgctctgcg
3361 aacggatcgg cggacgggcc atgatgatcg gcttcggcgt cgccgccgcc gtgctgacgg
3421 tgcccctcct gacggcgatg accggatggt tctggtcggt gctggccgtg caatgcgccg
3481 ggatgctcgt cctcaccgcg tacacgtcgg tctccggcgc catcaacgcc gaactgttcc
3541 cgcaggagct gagaggacgc ggcatcgggc ttccgtacgc ggcgtcggtg gcattgttcg
3601 gcggcaccgc gccctacgtg ggcacatggc tgaaatccat gggactgaac gacttcttcc
3661 cctggtacgt ggcagtgctg tgcctgctga cggccctgac ggcgatcggg ctgccgcggc
3721 cggtgcccga tacgtgcgac ggaccggcgt cgccggcgca gcatttgcct ccggacttcg
3781 agcggcatgc ctgattttcc gcctgtgtag gtgaaagtgg cattgcgtct gaggttcacc
3841 ggaggggcct ggaatccttc aggtcaattt caagacatac cctgttcaca ggtcggatga
3901 ccttgcctcg actggttgac tctctacact gaagatttac atctgattag tgcgccaagg
3961 gtgccgcacg tggagtttac ggggaggcgg cattgacttt tcatcaggtg gcgacgtatg
4021 agatcccctc gctgacggtc tccgatccca cgccctccga taccgccgac tcgcagacgg
4081 gggacgccga gggggccgtg acgcggctgc tgagcgccca ccgcaaagtg gtggcggatc
4141 agggccggct gctggaagcc ctggagtccg cgctcggcga gctggtgcgc agttcgccgt
4201 cgcgcaccga ctgctcgtcg ctcgaagcgg aacaatgcgc ggtgggctgt ttcgcgatgg
4261 atcccctcgt ggccgccgaa ggcgaggtgc tgctctgtct ggatccggcc gatctggagc
4321 aggaacgttt ctcctacctg ctcgagtgcc ttcatggagc cttcctccgg ggggtgacca
4381 tacggtgcat ctgcgcggag tcgatgatca acctgccggg tggccggtcg tacatccagg
4441 aactccagac ggcgggggcg gagatccggg tggcccccct gttgccgttc cggctcatgc
4501 tcgtggaccg gatattcgcc tgtgtcagtg ttgtcgaccg ccacggcgag aattcgacgc
4561 tggagatcag aagtccggaa acctgccatt ttgtgcaccg tgtattcgac tactgctggg
4621 tgacgagaac gtcgtcgaaa gcgtgcgaga cggccgatgc tattgatgtg tccgaccgtg
4681 aggttatcat tctgcgcttg ttggccaacg gcatgaagga cgtggccatg gcgcgttctc
4741 tcggaatttc cacgcggacc ctccggcggg tgataacgga tctcatgggc aagctcgggg
4801 tcagcagcag gtttcaactg ggagcacgtg ccgccgaatg caggttactg tggtcggtcc
4861 tttcctgagt tcgtggtgga ggtcagcacg gcaccggccg cgacgcaccc ggccagcccg
4921 aagaacgcga gggggtagct gctcagttcg gcggcgagca ccgctcccag ggcgggtgcc
4981 acggcgaggg ctgccgtggc
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