GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS YSCGCN2 4813 bp ds-DNA PLN 30-MAY-1991
DEFINITION S.cerevisiae GCN2 gene (which couples GCN4 expression to amino acid
availability), complete cds.
ACCESSION M27082
KEYWORDS GCN2 gene.
SOURCE S.cerevisiae (strain S288c) DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 4813)
AUTHORS Wek,R.C., Jackson,B.M. and Hinnebusch,A.G.
TITLE Juxtaposition of domains homologous to protein kinases and
histidyl-tRNA synthetases in GCN2 protein suggests a mechanism for
coupling GCN4 expression to amino acid availibility
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 4579-4583 (1989)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 27..4799
/function="Potentially couples GCN4 expression to amino
acid availability"
/gene="GCN2"
/codon_start=27
BASE COUNT 1667 a 771 c 964 g 1411 t
ORIGIN
1 aatcttctat aacattacat tttgcgatga ccccaatgta tccttatacc gctccagaaa
61 tagaattcaa aaatgtacaa aatgtaatgg atagtcaatt gcaaatgctg aaaagtgaat
121 ttaagaaaat ccacaacacc tcccgaggcc aagagattat atttgaaatt acatctttta
181 ctcaagaaaa actggacgaa tttcaaaatg tggtaaatac acagtccttg gaagatgatc
241 gattacaaag aatcaaagaa actaaagaac aattagaaaa ggaagaaaga gaaaaacaac
301 aggaaacaat aaaaaaaaga tcagatgagc agcgaaggat agatgaaatt gttcaaagag
361 agttggagaa aagacaagat gatgatgatg atttgctatt caacagaaca acccagttag
421 atttacaacc accttcagaa tgggttgcat caggtgaagc tattgtcttt tcaaaaacta
481 taaaggcaaa attgcctaat aactcaatgt tcaagtttaa agcagttgta aatcctaagc
541 caataaaact gacatcagat atatttagtt tttccaaaca atttcttgtg aagccttata
601 taccaccaga atctccgttg gcagattttt taatgtcttc tgaaatgatg gaaaatttct
661 actatttgct atctgaaatt gaattggata atagctattt caacacaagt aatggaaaaa
721 aagaaatagc aaatttagag aaagagttag agacggtgtt gaaagctaag catgacaatg
781 tgaatcgatt gttcggttat acggtggagc gcatggggag aaataatgca acgtttgttt
841 ggaaaataag actcttgaca gagtactgta actactatcc attgggagat ttgatacaat
901 ctgttggatt tgttaactta gcaacagcgc gtatttggat gattagattg cttgaaggat
961 tggaggccat acacaaattg ggaattgttc ataaatgtat caacttagaa accgtgatcc
1021 tggtgaagga tgcagatttt ggaagcacta ttcccaagtt agttcactcc acttatggct
1081 acactgtttt gaatatgcta tcgagatatc caaataaaaa tggttcttcg gttgagttat
1141 ctccaagtac atggatagcc cctgagttgt tgaaattcaa taacgccaaa cctcaaagat
1201 taactgatat ttggcaactt ggtgttttgt ttatccagat aatcagtgga tctgatatag
1261 tgatgaattt tgaaacgcct caagaattcc tagattcaac aagtatggat gaaactttat
1321 atgatcttct ttcgaaaatg cttaataacg atccgaagaa aagattagga acattagaac
1381 tactgcccat gaaattctta aggaccaata ttgactctac aatcaatcga tttaacttag
1441 tttccgaaag tgtcaattct aattccttgg agttaactcc tggagatacc ataaccgttc
1501 ggggcaatgg aggtagaaca ctttcacaat cgagtatacg aagaagatca tttaatgttg
1561 gttccagatt ctcttctata aatcctgcaa cgcgatcacg atatgcttct gactttgaag
1621 agattgcagt tttaggccag ggcgcatttg gacaagttgt caaggcacgt aatgctctcg
1681 atagcagata ctatgcgatc aagaagatta gacatacaga agaaaagtta tctactatat
1741 tgagtgaagt aatgctgtta gcaagcttaa atcatcaata tgttgtgcgt tactatgctg
1801 catggttaga agaagacagt atggatgaaa acgtttttga atcaactgat gaagaaagtg
1861 acttgagcga atcttcctct gattttgagg aaaatgattt attagatcaa agcagtattt
1921 ttaaaaatag aacaaatcac gatttggata atagtaactg ggatttcata tcggggtcag
1981 gatatccgga tattgtcttt gaaaatagtt ctcgtgatga tgaaaatgaa gatctagacc
2041 atgatacttc ctcgacttcc tcgagcgaaa gtcaagatga tactgataaa gaatcaaaga
2101 gtatccagaa cgttccaaga aggaggaatt ttgtaaaacc gatgactgct gttaagaaga
2161 aaagtacgct ttttattcaa atggagtact gtgaaaatag aacgctatat gatttgatcc
2221 attctgaaaa tttaaatcaa caacgtgatg aatattggag gttatttcga caaattttgg
2281 aagcactgag ttatatacat tcccagggta tcattcatag ggatctgaag ccaatgaata
2341 tttttataga tgaatcgaga aatgttaaaa tcggtgattt tgggttagct aagaacgtcc
2401 atagatctct ggatatactt aagctagatt cacagaattt gccaggcagc tcagataatt
2461 taacatccgc cattggtaca gcaatgtatg ttgctactga agttttagat ggtacaggtc
2521 actataatga aaagattgat atgtattcac ttggaatcat tttttttgaa atgatctatc
2581 ctttcagtac aggtatggag agagttaata ttttgaaaaa gttacgatca gtgtcgatag
2641 aatttcctcc tgatttcgac gataataaga tgaaagttga aaagaaaatt ataaggttac
2701 tcatagacca tgatcccaat aaaaggcctg gtgctaggac attattaaat agtggttggc
2761 ttcctgtgaa gcatcaggat gaagtaatca aagaggcttt aaaaagtttg tcgaatcctt
2821 catccccttg gcaacagcaa gttcgagaaa gtttatttaa ccaatcttac agtctaacaa
2881 atgatattct atttgataac tcagttccaa catccactcc tttcgcaaac attctcaggt
2941 cccaaatgac agaagaggta gttaaaattt tcaggaaaca tggaggaatt gaaaataatg
3001 ctcctccgag gatttttcca aaggccccca tatacggtac gcagaatgta tatgaagtgc
3061 ttgacaaggg cggtaccgtc ttgcagttac aatatgattt aacttatcct atggctaggt
3121 atctatctaa aaatccaagt ctgatttcta agcaatatag gatgcagcac gtttaccgac
3181 ctcctgatca ttcaaggtca agtttggaac ctagaaagtt tggtgagatt gacttcgaca
3241 taatttcaaa atcttcctca gagtcaggat tttatgatgc agaaagcttg aaaattatcg
3301 atgaaatatt aaccgtattt cctgtatttg agaaaacaaa cacttttttc atattaaatc
3361 atgctgatat tttggagagt gttttcaact ttacaaatat tgataaagcc caaaggcctc
3421 tagtttcacg aatgttgtcg caagtaggct ttgcaaggtc cttcaaggaa gtaaagaatg
3481 aactaaaggc gcaactgaac atatcttcta cggcattgaa tgatttggag ttatttgatt
3541 ttagactgga cttcgaagca gccaaaaaac gcctgtataa attgatgatt gatagtccgc
3601 atctaaaaaa aattgaggac tctttgtccc atatatcaaa ggttctcagt tacctaaaac
3661 ccttagaagt tgcaagaaat gttgtgatat ctcctttgag taactacaat agcgcttttt
3721 acaaaggagg tatcatgttt catgcagttt atgacgatgg atcctcacgt aatatgatag
3781 ctgctggagg gaggtatgac actttgatat ccttttttgc cagaccatca ggaaaaaaga
3841 gcagcaatac tcgtaaggct gtaggtttca acttagcgtg ggaaacaata ttcggtatag
3901 cccaaaacta tttcaaactc gcttctggaa ataggataaa gaagagaaat aggtttttga
3961 aagatacagc tgttgattgg aagccaagca ggtgtgatgt attgatatcg agtttttcga
4021 actctttgtt ggacacaatc ggggttacaa tactgaatac attgtggaag caaaacatta
4081 aagcggatat gttaagggat tgttcctcgg tggatgatgt cgttactggc gctcaacagg
4141 atggtataga ctggattttg ctgattaagc aacaagcgta tccactaacc aatcacaaga
4201 gaaagtacaa gccattaaaa ataaaaaaat tgagcactaa tgttgacata gatttagatc
4261 ttgatgagtt tttaaccttg taccaacaag aaactggtaa taaatctttg atcaacgata
4321 gtctcacttt gggcgataag gctgatgaat ttaaaagatg ggatgaaaac agcagtgccg
4381 gtagtagtca agaaggtgac atagatgatg ttgttgctgg ttcgactaat aatcaaaagg
4441 taatttatgt tccaaacatg gctacaagat ctaagaaagc taataaaagg gaaaagtggg
4501 tttatgagga tgcagccaga aattcttcga atatgatatt acacaattta tccaatgcac
4561 caattatcac tgttgatgcc ttaagagatg aaactttaga aataatctca attacttctt
4621 tggctcagaa ggaagaatgg ctgagaaaag tttttgggtc aggtaataac tcgactccta
4681 gaagctttgc cacgagcatt tataataacc tctccaaaga ggctcataaa gggaataggt
4741 gggcaatatt atactgccac aaaaccggaa aatcatctgt tatcgattta cagaggtagg
4801 cctttaaaga tct
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