GenBank-Updates@genbank.bio.net (02/27/91)
LOCUS ECOTNSAB 1201 bp ds-DNA BCT 27-FEB-1991
DEFINITION E.coli (strain K-12) tranposition protein A and B (tns A and tns B)
gene, complete cds and 5' end, respectively.
ACCESSION M37391
KEYWORDS transposition protein.
SOURCE E.coli (strain K-12) transposon Tn7 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1201)
AUTHORS Orle,K.A. and Craig,N.L.
TITLE Identification of transposition proteins encoded by the bacterial
transposon Tn7
JOURNAL Gene 96, 1-7 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
Univ Calif SF, George W. Hooper Foundation, 3rdand Parnassus,]
kindly submitted
by K.A.Orle, 02-AUG-1990.
FEATURES Location/Qualifiers
misc_binding 9..30
/gene="tns B"
misc_binding 29..50
/gene="tns B"
misc_binding 50..71
/gene="tns B"
misc_binding 70..92
/gene="tns B"
CDS 135..956
/product="transposition protein A"
/gene="tns A"
/codon_start=135
CDS 943..1201
/pseudo
/product="transposition protein B"
/gene="tns B"
/codon_start=943
/partial
BASE COUNT 364 a 207 c 276 g 354 t
ORIGIN
1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattccatgg ctaacagtac
241 aagaagttcc ttcttcaggt cgttcccacc gtatttattc tcataagacg ggacgagtcc
301 atcatttgct atctgactta gagcttgctg tttttctcag tcttgagtgg gagagcagcg
361 tgctagatat acgcgagcag ttccccttat tacctagtga taccaggcag attgcaatag
421 atagtggtat taagcatcct gttattcgtg gtgtagatca ggttatgtct actgattttt
481 tagtggactg caaagatggt ccttttgagc agtttgctat tcaagtcaaa cctgcagcgg
541 ccttacaagg cgagcgtacc ttagaaaaac tagaactaga gcgtcgctat tggcagcaaa
601 agcaaattcc ttggttcatt tttactgata aagaaataaa tcccgtagta aaagaaaata
661 ttgaatggct ttattcagtg aaaacagaag aagtttctgc ggagctttta gcacaactat
721 ccccattggc ccatatcctg caagaaaaag gagatgaaaa cattatcaat gtctgtaagc
781 aggttgatat tgcttatgat ttggagttag gccaaacatt gagtgagata cgagccttaa
841 ccgcaaatgg ttttattaag ttcaatattt ataagtcttt cagggcaaat aagtgtgcag
901 atctctgtat tagccaagta gtgaatatgg aggagttgcg ctatgtggca aattaatgag
961 gttgtgctat ttgataatga tccgtatcgc attttggcta tagaggatgg ccaagttgtc
1021 tggatgcaaa taagcgctga taaaggagtt ccacaagcta gggctgagtt gcttgctaat
1081 gcagtattta gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt
1141 agaagagccg tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat
1201 t
//GenBank-Updates@genbank.bio.net (02/27/91)
LOCUS ECOTNSD 164 bp ds-DNA BCT 27-FEB-1991
DEFINITION E.coli (strain K-12) transposition protein D (tns D) gene, 5' end.
ACCESSION M37393
KEYWORDS transposition protein.
SOURCE E.coli (strain K-12) transposon Tn7 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 164)
AUTHORS Orle,K.A. and Craig,N.L.
TITLE Identification of transposition proteins encoded by the bacterial
transposon Tn7
JOURNAL Gene 96, 1-7 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
Univ Calif SF, George W. Hooper Foundation, 3rdand Parsassus,]
kindly submitted
by K.A.Orle, 02-AUG-1990.
FEATURES Location/Qualifiers
CDS 48..>164
/product="transposition protein D"
/gene="tns D"
/codon_start=48
BASE COUNT 42 a 27 c 48 g 47 t
ORIGIN
1 ggggtaatag tggatatggc gagcttattt aaacaagcag gttagccatg agaaactttc
61 ctgttccgta ctcgaatgag ctgatttata gcactattgc acgggcgggc gtttatcaag
121 ggattgttag tcctaagcag ctgttggatg aggtgtatgg caac
//GenBank-Updates@genbank.bio.net (02/27/91)
LOCUS ECOTNSC 100 bp ds-DNA BCT 27-FEB-1991
DEFINITION E.coli (strain K-12) transposition protein C (tns C) gene, 5' end.
ACCESSION M37392
KEYWORDS transposition protein.
SOURCE E.coli (strain K-12) transposon Tn7 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 100)
AUTHORS Orle,K.A. and Craig,N.L.
TITLE Identification of transposition proteins encoded by the bacterial
transposon Tn7
JOURNAL Gene 96, 1-7 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)
Univ Calif SF, George W. Hooper Foundation, 3rdand Parnassus,]
kindly submitted
by K.A.Orle, 02-AUG-1990.
FEATURES Location/Qualifiers
CDS 25..>100
/product="transposition protein C"
/gene="tns C"
/codon_start=25
BASE COUNT 27 a 18 c 29 g 26 t
ORIGIN
1 ggatccacca gaaaaggatg agtcatgagt gctacccgga ttcaagcagt ttatcgtgat
61 acgggggtag aggcttatcg tgataatcct tttatcgagg
//GenBank-Updates@genbank.bio.net (03/28/91)
LOCUS ECOMIAA 1328 bp ds-DNA BCT 28-MAR-1991
DEFINITION E.coli (delta)2-isopentenyl pyrophosphate transferase (miaA) gene,
complete cds, and mutator protein (mutL) gene, 3' end.
ACCESSION M63655 M37459
KEYWORDS (delta)2-isopentenyl pyrophosphate transferase; miaA gene;
mutL gene; mutator protein.
SOURCE E.coli K-12 DNA, clone pNU127.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1328)
AUTHORS Winkler,M.E. and Connolly,D.M.
TITLE Structure of Escherichia coli K-12 miaA and characterization of the
mutator phenotype caused by miaA insertion mutations
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..63
/function="required for methyl-directed mismatch"
/product="mutator protein"
/gene="mutL"
/codon_start=1
/partial
CDS 56..1006
/product="(delta)2-isopentenyl pyrophosphate transferase"
/gene="miaA"
/codon_start=56
CDS 1093..1328
/note="ORF"
/codon_start=1093
/partial
BASE COUNT 317 a 297 c 382 g 332 t
ORIGIN 95 min. on K-12 map.
1 ccgggtggtc tgttacaatc tgttgattta catccggcga taaaagccct gaaagatgag
61 tgatatcagt aaggcgagcc tgcctaaggc gatttttttg atggggccga cggcctccgg
121 taaaacggcg ttagccattg agctgcgtaa aattttacca gtagagttga taagcgttga
181 ttctgccctt atttacaaag ggatggatat cgggacggcg aagccgaacg ctgaagagtt
241 actcgccgcg ccgcaccgat tgctggatat tcgcgatccg tcgcaggctt actcggctgc
301 tgattttcgc cgcgatgcgc tggcggaaat ggccgatatc accgcggcgg ggcggatccc
361 actgttagtg ggcggtacga tgttgtattt caaggcattg ctggaagggt tgtcgccgct
421 accgtcggca gacccggaag tacgggccag aattgagcaa caggcggcag agcaaggttg
481 ggagtcattg catcgtcaac ttcaggaggt agatccggtt gcggcagcaa ggattcatcc
541 aaatgatcca caaaggcttt cccgggcact ggaagttttt ttcatttcgg gtaaaacttt
601 aacggaactg acgcaaacgt caggagacgc tctaccgtat caggtgcatc agttcgccat
661 cgccccggcg agccgtgaac tgctccatca acgcattgag cagcgttttc atcagatgtt
721 ggcttcaggt tttgaagcag aagtccgggc gctttttgcc cgaggagatt tgcatacgga
781 cttgccttcc attcgttgcg tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat
841 ctcatacgat gaaatggttt atcgaggtgt ttgcgccacg agacagttgg cgaagcggca
901 gataacctgg ctgcgtggtt gggaaggggt tcactggctt gacagtgaaa aaccagaaca
961 ggcgcgtgac gaagtattac aggttgttgg tgctatcgca ggctgaatgt gtacaattga
1021 gacgtatcgt gcgcaatttt ttcaggaatc gaaaggttca aagtacaaat aagcatataa
1081 ggaaaagaga gaatggctaa ggggcaatct ttacaagatc cgttcctgaa cgcactgcgt
1141 cgggaacgtg ttccagtttc tatttatttg gtgaatggta ttaagctgca agggcaaatc
1201 gagtcttttg atcagttcgt gatcctgttg aaaaacacgg tcagccagat ggtttacaag
1261 cacgcgattt ctactgttgt cccgtctcgc ccggtttctc atcacagaac aacgccggtg
1321 gcggtacc
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECOGNDG 1687 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR4) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64324
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR4, individual_isolate RM39A) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA 87..1567
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
RBS 125..130
/gene="gnd"
CDS 137..1543
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=137
terminator 1568..1599
/note="factor independent terminator"
/gene="gnd"
BASE COUNT 421 a 416 c 438 g 412 t
ORIGIN 44 min. on K-12 map.
1 attttttgaa atgccatttt tttacgcata actggatttt gtaatatctc agtattaata
61 ttggtttaaa ttttattctg accatattca agccgcgtat accttgtgcg gcgaccacac
121 ctgacaggag tatgtaatgt ctaagcaaca gatcggcgtc gtcggtatgg cagtgatggg
181 gcgcaacctg gcgctcaaca tcgaaagccg aggttatacc gtctccatct tcaaccgctc
241 ccgcgagaaa actgaagaag ttgttgccga gaacccgggt aagaaactgg ttccttatta
301 cacggtgaaa gagttcgtcg agtctcttga aaccccacgt cgtatcctgt taatggtaaa
361 agcaggggcg ggaactgatg ctgctatcga ttccctgaag ccgtaccttg ataaaggcga
421 catcattatt gatggtggta acaccttctt ccaggacact atccgtcgta accgtgagct
481 gtcagcagaa ggttttaact tcatcggtac cggcgtgtcc ggtggtgaag aaggggcgct
541 gaaaggccca tctatcatgc ctggtggcca gaaagaagcg tatgagctgg ttgcgcctat
601 cctgaccaag attgccgctg ttgccgaaga tggtgaaccg tgcgtaacct acatcggcgc
661 tgatggcgca ggtcattatg tgaagatggt gcataacggt attgaatacg gcgacatgca
721 gctgattgct gaagcctatt ccttgctgaa aggcggcctg aacctgtcta acgaagagct
781 ggctaccacc ttcaccgagt ggaatgaagg cgagctgagc agctacctga tcgatatcac
841 taaagacatc ttcaccaaaa aagatgaaga cggtaactac ctggttgatg tgatcctgga
901 cgaagcggcg aacaaaggca ccggtaaatg gaccagccag agctctctgg atctgggtga
961 accactgtcg ctgatcaccg aatccgtatt cgcgcgctac atctcttctc tgaaagacca
1021 gcgcgttgcg gcatctaaag tgctgtccgg cccacaggct aaactggctg gtgataaagc
1081 agagtttgtt gagaaagttc gtcgcgcgct gtatttgggc aaaatcgtct cttatgccca
1141 gggtttctct caactgcgtg ccgcatctga cgaatacaac tgggatctga actacggtga
1201 aatcgcgaag atcttccgcg cgggctgcat cattcgcgcg cagttcctgc agaaaatcac
1261 cgacgcctat gccgaaaaca aaggtatcgc gaacctgctg ctggctccgt acttcaaaaa
1321 tatcgctgac gattatcagc aagcgctgcg tgatgtggtg gcttatgctg tgcagaacgg
1381 tattccggta ccgaccttct cagcagcggt agcctactac gacagctacc gcgctgcggt
1441 actgcctgct aacctgatcc aggcccagcg cgactacttt ggtgctcata cgtataaacg
1501 caccgataaa gaaggtgcgt tccacaccga atggctggat taatctgatt tagtctaact
1561 gtgaatccag gcccggagtg ctcctccggg cttttaattg ccaaaaaatg ggattcagtc
1621 aggtccgata tccggtgttg tacgaatcac gatatactgt cgatcgagag ccgatgtgcc
1681 agttgtt
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGH 1831 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR16) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64325
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR16, individual_isolate RM191F) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA 153..1676
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/partial
RBS 258..263
/gene="gnd"
CDS 270..1676
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=270
terminator 1680..1723
/note="potential terminator"
/gene="gnd"
BASE COUNT 475 a 397 c 438 g 521 t
ORIGIN 44 min. on K-12 map.
1 caattattta tttaggcgtg ggcttgtatt aaaaatgcat ctgcaaacat tcattccaag
61 agctggtaat agataatttt gacctcttgt attacagaat aaaggatttg ttgatttcgt
121 taaatatcaa ttgtatagtg ttgaaactat gcagttgata aattgaagtt tatattggcg
181 catttatcta tgtttagtaa tgcccagtcg tataatatcg aaatgagctg cgtacttgtc
241 gcggtgacca aaccagacag gagtatgtaa tgtctaagca acagatcggg gttgtcggta
301 tggcagttat ggggcgtaat ctagcgctca acatcgaaag ccgtggttat accgtctccg
361 ttttcaaccg ctcccgtgag aagaccgaag aagtgattgc tgaaaatcct ggcaagaaac
421 tggtccctta ctacacagta caagagtttg ttgaatccct tgaaacacca cgccgtatcc
481 tgttgatggt gaaggctggg gctggtacag atagcgccat cgattccctg aagccttacc
541 ttgataaagg cgacatcatc attgatggtg gcaacacctt cttccaggac accatccgtc
601 gtaaccgtga attgtctgcc gaaggtttta actttatcgg taccggtgtt tccggtggtg
661 aagagggggc tctgaaaggg ccttccatca tgcctggtgg gcagaaagaa gcctatgagc
721 tggttgctcc gattctgaag cagatcgcgg cggttgctga agacggtgag ccgtgtgtga
781 cttatatagg tgctgacggt gctggccatt atgtgaagat ggttcataac ggcatcgagt
841 atggtgacat gcagctgatt gctgaagctt atgcgctttt gaaaggtggt ctggcactat
901 ccaacgaaga gctggcgcaa acctttaccg aatggaacga aggtgaactg agcagctact
961 tgatcgacat tactaaagat atcttcacca aaaaggatga agagggtaac tacctcgttg
1021 atgttattct tgatgaagcc gcaaataaag gtaccggcaa gtggaccagc caaagctcac
1081 tggatctcgg cgaacctctg tctctgatca ccgagtctgt ttttgctcgt tatatctctt
1141 ctctgaaaga ccagcgtgta gccgcttcga aagtgctgag tggtccgcag gctacgccgg
1201 ccggtgataa agctgaattt atcgaaaaag ttcgtcgtgc gttgtacctc ggtaaaatcg
1261 tttcctatgc tcagggcttc tcccagctgc gtgctgcttc tgatgaatac aactgggatc
1321 ttaactacgg cgagatcgct aagattttcc gcgctggctg catcattcgt gcgcagttcc
1381 tgcagaagat caccgatgct tattcgcaaa acgctggcat tgctaacctc ctgctggcac
1441 cgtacttcaa acagatcgct gatgactatc agcaggcact gcgtgatgtc gtggcctatg
1501 ccgtgcagaa tggtattccg gtaccaacgt tctctgccgc aattgcctac tatgacagct
1561 accgttccgc tgttctgccc gctaacctga ttcaggctca gcgtgattac tttggtgcgc
1621 acacctataa gcgcactgat aaagaaggcg ttttccacac tgagtggtta gattaatatt
1681 tatattatta gaccttcata tctttattga aggtctaata ataaagcttg agcatcatca
1741 agatctatta tgataaattg cgaaaattaa gaatttcgct atttgttttt aaagtattgc
1801 ttcgatatta tcccataaac ttacatctca a
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGI 770 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR45) 6-phosphogluconate dehydrogenase (gnd) gene,
partial cds.
ACCESSION M64326
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR45, individual_isolate RM201C) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 1..770
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=3
/partial
BASE COUNT 194 a 179 c 206 g 191 t
ORIGIN 44 min. on K-12 map.
1 ctgcagaagg ctttaacttc attggtaccg gtgtttctgg cggtgaagaa ggtgcgctga
61 aaggtccttc cattatgcct ggtggacaga aagaagccta tgaactggtt gcgccgatcc
121 tgaccaaaat cgccgcagtg gctgaagacg gcgaaccgtg cgttacctat attggttccg
181 atggtgcagg tcattatgtg aagatggttc acaacggtat tgaatacggt gatatgcagt
241 tgattgccga agcctattct ctgctaaaag gtggcctgaa ccttaccaac gaagaactgg
301 cacagacctt tactgaatgg aataacggtg aactgagcag ctacctgatc gacatcacca
361 aagatatctt caccaaaaaa gatgaagatg gtaactacct ggttgatgtg attctggatg
421 aagcagcaaa taaaggtacg ggcaaatgga ccagccagag tgcgctggat ctcggcgaac
481 cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagatcagc
541 gcgttgccgc ttctaaagtt ctctctggcc cgcaagcgca gccagcaggc gataaggctg
601 agttcatcga aaaagttcgt cgtgcgctgt atctgggcaa aatcgtttct tacgctcagg
661 gcttctctca actgcgtgct gcgtctgaag agtacaactg ggatctgaac tacggcgaaa
721 tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGJ 770 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR67) 6-phosphogluconate dehydrogenase (gnd) gene,
partial cds.
ACCESSION M64327
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR67, individual_isolate RM217C) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 1..770
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=3
/partial
BASE COUNT 194 a 184 c 207 g 185 t
ORIGIN 44 min. on K-12 map.
1 ctgcagaagg ctttaacttc attggtaccg gtgtttccgg tggtgaagaa ggtgcgctga
61 aaggtccttc cattatgcct ggtggtcaga aagaagccta tgaactggtt gcgccgatcc
121 tgaccaaaat cgccgcagtg gctgaagacg gcgaaccgtg cgttacctat attggtgccg
181 atggtgcagg tcattatgtg aagatggttc acaacggtat tgaatacggc gatatgcagc
241 tgattgctga agcctattct ctgcttaaag gtggcctgaa tctcaccaac gaagaactga
301 cgcagacgtt taccgagtgg aataacggtg aactaagcag ctacctgatc gacattacca
361 aagacatctt cactaaaaaa gatgaagacg gtaactacct ggttgatgtg atcctggatg
421 aagcggctaa caaaggtacc ggtaaatgga ccagccagag cgcactggat ctcggcgaac
481 cgctgtcgct gattaccgag tctgtgtttg cacgatacat ctcttctctg aaagatcagc
541 gcgttgctgc gtctaaagtt ctctctggcc cacaagcgca gccagctggc aacaaggctg
601 agttcatcga aaaagttcgc cgtgcactgt atctgggcaa aatcgtttct tacgctcagg
661 ggttctctca actgcgtgcg gcgtctgaag agtacaactg ggatctgaac tacggcgaaa
721 tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGK 1787 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR69) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64328
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR69, individual_isolate RM45E) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
RBS 241..245
/gene="gnd"
CDS 252..1658
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=252
terminator 1686..1716
/note="rho-independent terminator"
/gene="gnd"
BASE COUNT 473 a 405 c 426 g 483 t
ORIGIN 44 min. on K-12 map.
1 ccaatagtac atatagttgg tgggggcatg gttaaatact ttcgaagata aaatagttgt
61 ttcccctcgt aagtggtttg ctggaaataa taaatctaag ttgaccatgg atagttggat
121 taatctttga ttatgctgtg tgtttttttt aaaatatgat atccattcat aatttgtaat
181 tttgatatat tatttttaga ctaaaatgtc aatctgcaac attccgtagt aatccctgac
241 aggagtaaac aatgtcaaag caacagatcg gcgtagtcgg tatggcagtg atggggcgta
301 accttgcgct caacatcgaa agccgtggtt ataccgtctc tattttcaac cgctcccgtg
361 agaaaacgga agaagtaatt gccgaaaacc cgggcaagaa actggttcct tactatacgg
421 tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg gtgaaagcag
481 gtgcaggcac ggatgctgct attgattccc tcaagccata cctcgataaa ggtgacatca
541 tcattgatgg cggtaacacc ttcttccagg acaccattcg tcgtaaccgt gagctttctg
601 ccgaaggctt taacttcatt ggtaccggtg tttccggtgg tgaagagggc gcgctgaaag
661 gtccttccat tatgcctggt ggccagaaag aagcctatga actggttgct ccgatcctga
721 ccaaaatcgc cgcagtagct gaagatggtg agccatgcgt tacctatatt ggtgccgatg
781 gcgcaggtca ctatgtgaag atggttcaca acggtattga atacggtgat atgcagctga
841 ttgctgaagc ctattctctg cttaaaggtg gcctgaatct ctctaacgaa gaactggcac
901 agacctttac cgagtggaat aacggtgaac tgagcagcta cctgatcgac atcaccaaag
961 atatcttcac caaaaaagat gaagacggta actacctggt tgatgtgatt ctggatgaag
1021 cagcaaacaa aggtaccggt aaatggacca gccagagcgc gctggatctc ggcgaaccgc
1081 tgtcgctgat taccgagtct gtgtttgcac gttatatctc gtctctgaaa gatcagcgcg
1141 ttgccgcgtc taaagttctc tctggcccgc aagcacagcc agcaggcgac aaggctgagt
1201 tcatcgaaaa agttcgccgt gcgcagtatc ttggcaaaat cgtttcttac gctcagggct
1261 tctctcagct gcgtgctgcg tctgaagaat acaactgggc tctgaactac ggcgaaatcg
1321 cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt cctgcagaaa atcaccgatg
1381 cttatgccga aaatccgcag atcgctaacc tgctgctggc tccgtacttc aagcaaattg
1441 ccgatgacta ccagcaggcg ctgcgtgatg tcgtcgcata tgcagtacag aacggtatcc
1501 cggttccgac cttcgccgct gcggttgcct attacgatag ctaccgtgcc gctgttctgc
1561 ctgcgaacct gatccaggca cagcgtgact atttcggtgc gcatacttat aagcgcattg
1621 ataaagaagg tgtgttccat actgaatggc tggattaatc tgatttaacc aacaataaaa
1681 ttgaggcccg gcgtatattg caccgggctt ttttttgcca attatcttat agactaaatt
1741 tcactgctta atattaactt aataaatatc agctatctat aagaaat
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGL 1879 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR70) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64329
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR70, individual_isolate RM70B) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA (278.311)..1789
/note="promoter and mRNA"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
RBS 347..351
/gene="gnd"
CDS 358..1764
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=358
terminator 1790..1820
/note="rho-independent terminator"
/gene="gnd"
BASE COUNT 496 a 408 c 436 g 539 t
ORIGIN 44 min. on K-12 map.
1 tttaatacct cattacaatg ctgagggttt gatgtatagt atcattatat ctcaattctt
61 tacatgcttt atttataacc ctaaacgtgt tataaataaa attggattag ataaagttaa
121 ttatattaaa agattaatgg tgcacttttt gtttatgttg actcttattt ttatggggtt
181 atattttcaa aatgatgata tattgccgag agttgtgatt ctttctttta tcacattgat
241 tggtgtttgt tttctctacc caaatgtgaa aaaaataatt gttgatttct tgtagttttt
301 taattttatt tgcattttaa atggaaaatc ttgttctatc ttagaaagga gttaacaatg
361 ctgaagcaac agatcggcgt agtcggtatg gccgtgatgg ggcgcaacct tgcgctcaac
421 atcgaaagcc gtggttatac cgtctctatt tacaaccgtt cccgtgaaaa gacggaagaa
481 gtgattgccg aaaatccagg caagaaactg gttccttact atacggtgaa agagtttgtt
541 gaatctctgg aaacgcctcg tcgcatcctg ttaatggtga aagcaggtgc aggcacggat
601 gctgctattg attccctcaa gccgtacctc gataaaggtg acatcatcat tgatggtggt
661 aacaccttct tccaggacac cattcgtcgt aaccgtgagc tttcttcaga aggctttaac
721 ttcatcggta ccggtgtttc cggcggtgaa gagggggcgc tgaaagggcc gtccatcatg
781 cctggtggcc agaaagaagc ctatgaactg gttgcaccga tcctgaccaa aatcgccgca
841 gtagctgaag acggggagcc atgcgttacc tatattggtg ccgatggtgc aggtcactat
901 gtgaagatgg ttcacaacgg tattgaatac ggcgatatgc agctgattgc tgaagcctat
961 tctctgctta aaggtggcct gaacctcacc aacgaagaac tggcacagac ctttaccgag
1021 tggaataacg gtgaactgag tagctacctg atcgacatca ccaaagacat cttcaccaaa
1081 aaagatgaag acggtaacta cctggttgat gtgatcctgg atgaagcggc taacaaaggt
1141 accggtaaat ggaccagcca gagcgcgctg gatctcggcg aaccgctgtc gctgattacc
1201 gagtctgtgt ttgcacgtta tatctcttct ctgaaagagc agcgtgttgc cgcatctaaa
1261 gttctctcag gcccgcaagc gcagccagct ggcgacaaag gtgagttcat tgaaaaagtt
1321 cgtcgtgctc tgtatctagg caaaatcgtt tcttacgctc agggtttctc tcagctgcgc
1381 gcagcgtctg aagagtacaa ctgggatctg aactacggcg aaatcgcgaa gattttccgt
1441 gctggctgca tcatccgtgc gcagttcctg cagaaaatca ccgatgcata tgccgaaaat
1501 ccgcagatcg ctaacctgct gctggccccg tacttcaagc aaattgccga tgactaccag
1561 caggcgctgc gcgatgtcgt tgcttatgca gtacagaacg gtatcccggt tccgaccttc
1621 gccgctgcgg ttgcctatta cgatagctac cgtgccgctg ttctgcctgc gaacctgatc
1681 caggcacagc gtgactattt cggtgcacat acttataagc gcattgataa agaaggtgtg
1741 ttccatactg aatggctgga ttaatctgat ttaaccaaca taaaattagg cccggcgtat
1801 attgcaccgg gctttttttt gccatttatc ttatagacta aatttcattg cttaatatta
1861 acttaataaa tatcagcta
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGM 1747 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR68) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64330
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR68, individual_isolate RM224H) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA 172..1653
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
RBS 211..215
/gene="gnd"
CDS 222..1628
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=222
terminator 1654..1683
/note="factor independent terminator"
/gene="gnd"
BASE COUNT 450 a 417 c 419 g 461 t
ORIGIN 44 min. on K-12 map.
1 caggagatat acatagtgtc aaatgcttgt caccaaaagc ccttatgttg gagataaagg
61 aggggccatt tgacccactg aaagctaagg ctttttctaa gtggttataa agcgatatac
121 caccgtttat tcttctatct tattctatac atgctgggtt accatcttag cttcttcaag
181 ccgcgtaacc ccgcggtgac cccccctgac aggagtaaac aatgtcaaag caacagatcg
241 gcgtcgtcgg tatggcagtg atgggacgca accttgcgct caacatcgaa agccgtggtt
301 ataccgtctc tattttcaac cgttcccgtg agaagacgga agaagtgatt gccgaaaatc
361 caggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct ctggaaacgc
421 ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattccc
481 tcaagccata cctcgataaa ggtgacatca tcattgatgg tggtaatacc ttcttccagg
541 acaccattcg tcgtaaccgc gagctttctg ccgaaggctt taacttcatt ggtaccggtg
601 tttccggtgg tgaagaaggg gcgctgaaag gcccttccat tatgcctggt gggcagaaag
661 aagcctatga attggttgcg ccgatcctga ccaaaatcgc cgccgttgct gaagacggcg
721 agccatgcgt tacctatatt ggtgccgatg gtgctggtca ctatgtgaag atggttcata
781 acggtattga atacggcgat atgcagctga ttgctgaagc ctattcactg cttaaaggtg
841 gcctgagcct caccaacgaa gaactggctc agacctttac cgaatggaat aacggtgaac
901 taagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat gaagacggta
961 actacctggt tgatgtgatt ttggatgaag cagcaaacaa aggtacaggc aaatggacca
1021 gccagagtgc gctagatctc ggcgaaccgc tgtcgctgat taccgagtct gtgtttgcac
1081 gttatatctc ttctctgaaa gatcagcgtg ttgccgcatc taaagttctc tctggcccgc
1141 aaggacagcc agcaggcgac aaggctgagt tcatcgaaaa agttcgccgt gcgctgtatc
1201 ttggcaaaat cgtttcttac gctcagggct tctctcagct gcgtgctgcg tctgaagagt
1261 acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc
1321 gtgcccagtt cctgcagaaa atcaccgatg cttatgccga aaatccgcaa atcgctaacc
1381 tgttgctggc tccttacttc aagcaaattg ccgatgacta ccagcaggcg ctgcgtgatg
1441 tcgttgctta tgcagtacaa aatggtatcc cggttccgac cttcgctgct gcggttgcct
1501 attatgatag ctaccgtgcc gctgttttgc ctgcgaacct gatccaggca cagcgtgact
1561 atttcggtgc gcatacttat aagcgcattg ataaagaagg tgtgttccat accgaatggc
1621 tggattaatc tgatttaacc aacaataaat tgaaacccgg cgtatattgc gccggttttt
1681 tttgccgatt atcttataga ctaaatttca ctgcttaata ttaacttaat aaatatcagc
1741 tattctt
//GenBank-Updates@genbank.bio.net (04/24/91)
LOCUS ECONDGN 1790 bp ds-DNA BCT 24-APR-1991
DEFINITION E.coli (strain ECOR65) 6-phosphogluconate dehydrogenase (gnd) gene,
complete cds.
ACCESSION M64331
KEYWORDS 6-phosphogluconate dehydrogenase; gnd gene.
SOURCE E.coli (strain ECOR65, individual_isolate RM202I) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Dykhuizen,D.E. and Green,L.
TITLE Recombination in Escherichia coli and the biological species
definition
JOURNAL Unpublished (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA (160.196)..1654
/note="promoter and mRNA sequence."
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
RBS 211..215
/gene="gnd"
CDS 222..1628
/EC_number="1.1.1.44"
/product="6-phosphogluconate dehydrogenase"
/gene="gnd"
/codon_start=222
terminator 1655..1687
/note="factor independent terminator"
/gene="gnd"
BASE COUNT 484 a 406 c 424 g 476 t
ORIGIN 44 min. on K-12 map.
1 ccaatagatt ttaatcttaa atattattat tttcagcacg gaacaaaaga tgaaattaat
61 aaattaattg aaatgggtga gattacataa ttaatattgt tactaacagc atatgttatt
121 taactccatc cttataatat aaatctcaat gcttatttct tacgttatga atattcttcg
181 taattgattt taaatttgta tgccaaaaac aggagtaaac aatgtcaaag caacagatcg
241 gcgtcgtcgg tatggcagtg atggggcgca accttgcgct caacatcgaa agccgtggtt
301 ataccgtctc tattttcaac cgttcccgtg agaagacgga agaagtgatt gccgaaaatc
361 caggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct ctggaaacgc
421 ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct attgattccc
481 tcaaaccata tctcgataaa ggtgacatca tcattgatgg cggtaagacc ttcttccagg
541 acaccattcg tcgtaaccgt gagctttctg cggaaggctt taactttatc ggtactggtg
601 tttccggtgg tgaagagggc gcactaaaag gtccttccat tatgcctggt ggccagaaag
661 aagcctatga attggttgct ccgatcctaa ccaaaatcgc cgcagtggct gaagacggcg
721 agccatgcgt tacctatatt ggtgccgatg gtgcaggtca ctatgtgaag atggttcaca
781 acggtattga atacggcgat atgcagctga ttgctgaagc ctattctctg cttaaaggtg
841 gcctgaatct caccaacgaa gaactggcgc agacctttac cgagtggaat aacggtgaac
901 tgagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat gaagacggta
961 actacctggt ggatgtgatc ctggatgaag cggcaaacaa aggtacgggc aaatggacca
1021 gccagagcgc gctggatctc ggtgaaccac tgtcgcttat taccgagtct gtgtttgcac
1081 gttatatctc gtctctgaaa gatcagcgcg ttgccgcgtc taaagttctc tctggcccgc
1141 aagcgcagcc agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt gctctgtatc
1201 tgggcaaaat cgtttcttac gcccaaggct tctctcagct gcgtgctgcg tctgaagagt
1261 acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc
1321 gtgcgcagtt cctgcagaaa atcaccgatg catatgccga aaatccgcag atcgctaacc
1381 tgctgctggc cccgtacttc aagcaaatcg ccgatgacta tcagcaggcg ctgcgtgatg
1441 tcgttgctta tgcagtacag aacggtatcc cggttcctac ctttgctgct gcggttgctt
1501 attatgacag ctaccgcgcc gctgttctgc ctgcgaacct gatccaggca cagcgtgact
1561 atttcggtgc gcatacttat aagcgcattg ataaagaagg tgtgttccat accgaatggc
1621 tggattaatc tgatttaaat cgattaatca atgcaaggcc cggagaaacc ctccgggctt
1681 ttttattata caaagcggca ggttatggcc tttttttata atttatgatt aaaaagcgat
1741 atatacagag ccgcacagca ggatcgtgcc tgacagtcat ctacatcagg
//GenBank-Updates@genbank.bio.net (05/08/91)
LOCUS ECOMIAA 1328 bp ds-DNA BCT 08-MAY-1991
DEFINITION E.coli (delta)2-isopentenyl pyrophosphate transferase (miaA) gene,
complete cds, and mutator protein (mutL) gene, 3' end.
ACCESSION M63655 M37459
KEYWORDS (delta)2-isopentenyl pyrophosphate transferase; miaA gene;
mutL gene; mutator protein.
SOURCE E.coli K-12 DNA, clone pNU127.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1328)
AUTHORS Winkler,M.E. and Connolly,D.M.
TITLE Structure of Escherichia coli K-12 miaA and characterization of the
mutator phenotype caused by miaA insertion mutations
JOURNAL J. Bacteriol. 173, 1711-1721 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..63
/function="required for methyl-directed mismatch"
/product="mutator protein"
/gene="mutL"
/codon_start=1
/partial
CDS 56..1006
/product="(delta)2-isopentenyl pyrophosphate transferase"
/gene="miaA"
/codon_start=56
CDS 1093..1328
/note="ORF"
/codon_start=1093
/partial
BASE COUNT 317 a 297 c 382 g 332 t
ORIGIN 95 min. on K-12 map.
1 ccgggtggtc tgttacaatc tgttgattta catccggcga taaaagccct gaaagatgag
61 tgatatcagt aaggcgagcc tgcctaaggc gatttttttg atggggccga cggcctccgg
121 taaaacggcg ttagccattg agctgcgtaa aattttacca gtagagttga taagcgttga
181 ttctgccctt atttacaaag ggatggatat cgggacggcg aagccgaacg ctgaagagtt
241 actcgccgcg ccgcaccgat tgctggatat tcgcgatccg tcgcaggctt actcggctgc
301 tgattttcgc cgcgatgcgc tggcggaaat ggccgatatc accgcggcgg ggcggatccc
361 actgttagtg ggcggtacga tgttgtattt caaggcattg ctggaagggt tgtcgccgct
421 accgtcggca gacccggaag tacgggccag aattgagcaa caggcggcag agcaaggttg
481 ggagtcattg catcgtcaac ttcaggaggt agatccggtt gcggcagcaa ggattcatcc
541 aaatgatcca caaaggcttt cccgggcact ggaagttttt ttcatttcgg gtaaaacttt
601 aacggaactg acgcaaacgt caggagacgc tctaccgtat caggtgcatc agttcgccat
661 cgccccggcg agccgtgaac tgctccatca acgcattgag cagcgttttc atcagatgtt
721 ggcttcaggt tttgaagcag aagtccgggc gctttttgcc cgaggagatt tgcatacgga
781 cttgccttcc attcgttgcg tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat
841 ctcatacgat gaaatggttt atcgaggtgt ttgcgccacg agacagttgg cgaagcggca
901 gataacctgg ctgcgtggtt gggaaggggt tcactggctt gacagtgaaa aaccagaaca
961 ggcgcgtgac gaagtattac aggttgttgg tgctatcgca ggctgaatgt gtacaattga
1021 gacgtatcgt gcgcaatttt ttcaggaatc gaaaggttca aagtacaaat aagcatataa
1081 ggaaaagaga gaatggctaa ggggcaatct ttacaagatc cgttcctgaa cgcactgcgt
1141 cgggaacgtg ttccagtttc tatttatttg gtgaatggta ttaagctgca agggcaaatc
1201 gagtcttttg atcagttcgt gatcctgttg aaaaacacgg tcagccagat ggtttacaag
1261 cacgcgattt ctactgttgt cccgtctcgc ccggtttctc atcacagaac aacgccggtg
1321 gcggtacc
//GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOFAEDEF 902 bp ds-DNA BCT 25-MAY-1991
DEFINITION E.coli (plasmid pFM205) faeE and faeF genes
ACCESSION X56003
KEYWORDS chaperonin-like protein; faeE gene; faeF gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 902)
AUTHORS Oudega,B.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Bakker,D., Vader,C.E., Roosendaal,B., Stegehuis,F., Mooi,F.R.,
Oudega,B. and de,G.F.K.
TITLE Structure and function of periplasmic chaperone-like proteins
involved in the biosynthesis of K88 and K99 fimbriae in
enterotoxigenic Escherischia coli
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT *source: strain=wild type; See also <X03675,X56001>;
From EMBL 26 entry ECFAEDEF; dated 25-MAR-1991.
FEATURES Location/Qualifiers
mat_peptide <1..57
/partial
/product="FaeD"
/gene="faeD"
prim_transcript <1..>60
/partial
/gene="faeD"
prim_transcript <53..>829
/gene="faeE"
prim_transcript <864..>902
/partial
/gene="faeF"
CDS <1..60
/partial
/product="FaeD"
/gene="faeD"
/codon_start=1
RBS 41..46
/note="faeE ribosome binding site"
sig_peptide 53..109
CDS 53..829
/product="FaeE"
/gene="faeE"
/codon_start=53
mat_peptide 110..826
/product="FaeE"
/gene="faeE"
RBS 851..855
/note="faeF ribosome binding site"
mat_peptide 864..>902
/partial
/product="FaeF"
/gene="faeF"
CDS 864..>902
/partial
/product="FaeF"
/gene="faeF"
/codon_start=864
BASE COUNT 252 a 216 c 272 g 162 t
ORIGIN
1 cggatacctg cggccagact gcaggatact gagaaactac aggagattac gtgtgagtaa
61 gcgtaacgca gtaacgacgt ttttcactaa ccgggtgaca aaagcactgg gaatgactct
121 ggcgctgatg atgacctgtc agagtgcgat ggcttccctg gcagtagacc agacccgcta
181 tatctttcgc ggggacaagg atgcactgac catcacagtc accaacaatg ataaagagcg
241 tacctttggt ggtcaggcct gggtggacaa tatcgtggag aaggacaccc gtccgacctt
301 tgtggtgaca ccatccttct tcaaggtgaa gccgaatggt cagcagacac tgcgtatcat
361 catggcctcg gaccatctgc cgaaggataa agagtcggtg tactggctga acctgcagga
421 tattccgccg gctctggagg gcagcggtat tgcagtggcg ctgcgcacga agctgaaact
481 gttctaccgc ccgaaggcac tgcttgaagg ccgcaagggg gcagaagaag gcatcagcct
541 gcagagccgc ccggatggca ggaccatgct ggtgaacacc acgccgtaca tttttgcgat
601 tggcagtctg ctggacggaa acggaaagaa aattgccacg gataacggga cgacgcagaa
661 actgctgatg ttcatgccgg gggatgaagt gcaggtgaag ggaaatgtgg tgaaagtgga
721 ctccctgaac gattacggtg aactgcagac ctggacgatt aacaagaaga aaccggcagc
781 accggaagcc gcaaaagctg agaaagcaga taccgcagag cagaaataac cgccctccgg
841 aatacggaac agaggggtaa taaatgaaga aaacaatgat ggcagccgcc ctggttctga
901 gt
//GenBank-Updates@genbank.bio.net (05/25/91)
LOCUS ECOFANDEF 762 bp ds-DNA BCT 25-MAY-1991
DEFINITION E.coli (plasmid pFK99) fanD (partial) fanE and fanF (partial) genes
ACCESSION X56001
KEYWORDS chaperonin-like protein; fanD gene; fanE gene; fanF gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Bakker,D., Vader,C.E., Roosendaal,B., Stegehuis,F., Mooi,F.R.,
Oudega,B. and de,G.F.K.
TITLE Structure and function of periplasmic chaperone-like proteins
involved in the biosynthesis of K88 and K99 fimbriae in
enterotoxigenic Escherischia coli
JOURNAL Unpublished (1990)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 762)
AUTHORS Oudega,B.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: strain=wild type isolate B41; See also <X03675,X56003>;
From EMBL 26 entry ECFANDEF; dated 25-MAR-1991.
FEATURES Location/Qualifiers
mat_peptide <1..42
/partial
/product="FanD"
/gene="fanD"
prim_transcript <1..>45
/partial
/gene="fanD"
prim_transcript <38..>724
/gene="fanE"
prim_transcript <724..>762
/partial
/gene="fanF"
CDS <1..45
/partial
/product="FanD"
/gene="fanD"
/codon_start=1
RBS 26..31
/note="fanE ribosome binding site"
sig_peptide 38..94
/product="FanE"
CDS 38..724
/product="FanE"
/gene="fanE"
/codon_start=38
mat_peptide 95..721
/product="FanE"
/gene="fanE"
RBS 713..717
/note="fanF ribosome binding site"
mat_peptide 724..>762
/partial
/product="FanF"
/gene="fanF"
CDS 724..>762
/partial
/product="FanF"
/gene="fanF"
/codon_start=724
BASE COUNT 269 a 114 c 142 g 237 t
ORIGIN
1 ctaaaagata atttatcaga aattcaggag gtaaactgtg aataaattta tttctattat
61 agctctctgt gtattcagct cttacgctaa tgccgctttt actctcaata gtacgcggta
121 tatatataat gaaggacagc aaagtgtatc tgttaacatc cataacgaaa gtgaacataa
181 atatggtggt caggtatgga ttgataatat agacaagaat ggagaggtcg tatttttttc
241 tccaagtcca atggtgttca aattgaaccc gaaacagaag caaatagttc gcattgttaa
301 tattaatgat aatttaccta aagacagaga atcgattttc tggcttaatg ttcaggagat
361 ccctccagct ccaaagggag atgggggtag tctgtcactg gcaattaata atcgagtaaa
421 attaatatat cgaccaattg ctctaaaaaa tggtcgagat gaggcagaaa ataatattaa
481 gctgattaac tcgggcacgg attcttgcct tgaaaataca acgccatatt actttgcaat
541 tagtgatgtt aaaattaatg gcaaatcaat tgatttaaat tctgatgcaa aaaataagat
601 gggagtattc tccccattct cgaaagtttg tctgggaaat gtaaatacta gtggaaacat
661 cacggtaaca gcatttaatg actatggcgt tgcaaccagc tacactgttc aaaggagtaa
721 ataatgaaaa ataaatataa tttattattt tttttatttc tt
//GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS ECOMIAA 1328 bp ds-DNA BCT 30-MAY-1991
DEFINITION E.coli (delta)2-isopentenyl pyrophosphate transferase (miaA) gene,
complete cds, and mutator protein (mutL) gene, 3' end.
ACCESSION M63655 M26956 M37459
KEYWORDS (delta)2-isopentenyl pyrophosphate transferase; IPP transferase;
miaA gene; mutL gene; mutator protein.
SOURCE E.coli K-12 DNA, clone pNU127.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Connolly,D.M. and Winkler,M.E.
TITLE Genetic and physiological relationships among the miaA gene,
2-methylthio-n-6-(delta-2-isopentenyl)-adenosine tRNA modification,
and spontaneous mutagenesis in Escherichia coli K-12
JOURNAL J. Bacteriol. 171, 3233-3246 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1328)
AUTHORS Winkler,M.E. and Connolly,D.M.
TITLE Structure of Escherichia coli K-12 miaA and characterization of the
mutator phenotype caused by miaA insertion mutations
JOURNAL J. Bacteriol. 173, 1711-1721 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..63
/function="required for methyl-directed mismatch"
/product="mutator protein"
/gene="mutL"
/codon_start=1
/partial
CDS 56..1006
/product="(delta)2-isopentenyl pyrophosphate transferase"
/gene="miaA"
/codon_start=56
CDS 1093..1328
/note="ORF"
/codon_start=1093
/partial
BASE COUNT 317 a 297 c 382 g 332 t
ORIGIN 95 min. on K-12 map.
1 ccgggtggtc tgttacaatc tgttgattta catccggcga taaaagccct gaaagatgag
61 tgatatcagt aaggcgagcc tgcctaaggc gatttttttg atggggccga cggcctccgg
121 taaaacggcg ttagccattg agctgcgtaa aattttacca gtagagttga taagcgttga
181 ttctgccctt atttacaaag ggatggatat cgggacggcg aagccgaacg ctgaagagtt
241 actcgccgcg ccgcaccgat tgctggatat tcgcgatccg tcgcaggctt actcggctgc
301 tgattttcgc cgcgatgcgc tggcggaaat ggccgatatc accgcggcgg ggcggatccc
361 actgttagtg ggcggtacga tgttgtattt caaggcattg ctggaagggt tgtcgccgct
421 accgtcggca gacccggaag tacgggccag aattgagcaa caggcggcag agcaaggttg
481 ggagtcattg catcgtcaac ttcaggaggt agatccggtt gcggcagcaa ggattcatcc
541 aaatgatcca caaaggcttt cccgggcact ggaagttttt ttcatttcgg gtaaaacttt
601 aacggaactg acgcaaacgt caggagacgc tctaccgtat caggtgcatc agttcgccat
661 cgccccggcg agccgtgaac tgctccatca acgcattgag cagcgttttc atcagatgtt
721 ggcttcaggt tttgaagcag aagtccgggc gctttttgcc cgaggagatt tgcatacgga
781 cttgccttcc attcgttgcg tgggttatcg ccagatgtgg tcttaccttg aaggcgaaat
841 ctcatacgat gaaatggttt atcgaggtgt ttgcgccacg agacagttgg cgaagcggca
901 gataacctgg ctgcgtggtt gggaaggggt tcactggctt gacagtgaaa aaccagaaca
961 ggcgcgtgac gaagtattac aggttgttgg tgctatcgca ggctgaatgt gtacaattga
1021 gacgtatcgt gcgcaatttt ttcaggaatc gaaaggttca aagtacaaat aagcatataa
1081 ggaaaagaga gaatggctaa ggggcaatct ttacaagatc cgttcctgaa cgcactgcgt
1141 cgggaacgtg ttccagtttc tatttatttg gtgaatggta ttaagctgca agggcaaatc
1201 gagtcttttg atcagttcgt gatcctgttg aaaaacacgg tcagccagat ggtttacaag
1261 cacgcgattt ctactgttgt cccgtctcgc ccggtttctc atcacagaac aacgccggtg
1321 gcggtacc
//