GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS RATGLUT 4526 bp ss-mRNA ROD 30-MAY-1991
DEFINITION Rat glutaminase mRNA, complete cds.
ACCESSION M65150
KEYWORDS glutaminase; isozyme.
SOURCE Rat (Sprague-Dawley) adult, cDNA to mRNA.
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 4526)
AUTHORS Shapiro,R.A., Farrell,L., Srinivasan,M. and Curthoys,N.P.
TITLE Isolation, characterization, and in vitro expression of a cDNA that
encodes the kidney isozyme of the mitochondrial glutaminase
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 59..2083
/EC_number="3.5.1.2"
/product="glutaminase"
/evidence=EXPERIMENTAL
/codon_start=59
polyA_signal 2984..2989
polyA_signal 4510..4515
BASE COUNT 1171 a 1016 c 1070 g 1269 t
ORIGIN chromosome 9
1 gaattccgcg gacgcccacg ccccgagcat cctcttctga cgagcgggcc gcggcggcat
61 gatgcggctg cgaggctcgg cgatgctgcg ggagctgctc ttgaggccgc ctgccgccgt
121 cggaggcgtc ttgcggcgca cgcagccccc tggaccgctg tgccggcgcc cccggggcgg
181 gagccggccg gccgctggcc tggtggccgc tgcgcgactc cacccgtggt ggggcggggg
241 cggccgcgcg aagggcccgg gctcgggcgg cctgtccagc tcgccctcgg agatcctgca
301 ggagctgggc aaggggggca cgccgccaca gcagcagcag cagcagcagc agcagccagg
361 ggcgtcgcca cccgcagccc cgggccccaa ggacagcccg ggggagacgg acgcgttcgg
421 caacagcgaa ggcaaggaga tggtggctgc gggcgacaat aaagtaaagc agggtctgtt
481 acctagcttg gaagatttgc tgttctatac aattgcagaa ggacaagaaa agatacctgt
541 tcacaagttt attacagcac tcaaatctac aggattgcga acatctgatc ccaggttgaa
601 agagtgtatg gatatgttaa gattaactct tcagacaacg tcagatggtg tcatgctaga
661 caaagatctt tttaaaaagt gtgttcaaag caacatcgtt ttgttaacac aagcatttag
721 aagaaagttt gtcatccctg actttatgtc ttttacctca cacatcgatg agttatatga
781 aagtgctaaa aagcagtctg gagggaaggt tgctgattat attcctcaac tggccaaatt
841 cagtcctgat ttgtggggtg tatctgtttg taccgtagat gggcaaaggc attctattgg
901 agataccaaa gttccctttt gtcttcagtc ctgtgtaaag cccctgaaat atgcaattgc
961 tgtcaatgac ctgggaactg agtatgtgca tcgctatgtt gggaaggagc caagtggatt
1021 aagattcaac aagctctttt tgaatgaaga tgataaacca cataatccga tggtaaatgc
1081 tggagcaatt gttgtgactt cactaataaa gcaaggagta aataatgctg agaaatttga
1141 ctatgtgatg caatttttga ataagatggc tggtaatgaa tatgtcggat tcagtaatgc
1201 aacgtttcaa tctgaacgag aaagtggaga ccgaaatttt gcaataggat attacttaaa
1261 agaaaagaag tgttttccag aaggcacaga catggttggg atactagatt tttatttcca
1321 gctctgttcc attgaagtga cgtgtgagtc agcaagtgtg atggctgcaa ccttggctaa
1381 tggcggtttc tgcccaatta ctggtgaaag agtgctcagt cctgaggccg ttcggaacac
1441 cctgagctta atgcactcct gtggcatgta tgacttctca gggcagtttg catttcatgt
1501 tggtcttcct gcaaaatctg gagttgctgg gggtattctt ttagttgtcc ccaacgtcat
1561 gggcatgatg tgttggtctc ctcctcttga caagatgggc aacagtgtta aaggaattca
1621 cttttgtcac gatcttgttt ctctgtgtaa cttccataac tatgataatt tgagacactt
1681 tgcaaaaaaa cttgatcctc ggagagaagg aggtgatcaa agggtaaagt cggtgataaa
1741 cctcctgttt gctgcgtaca ctggagatgt gtctgccctc cgaaggtttg ctctgtcagc
1801 catggacatg gaacagagag attacgactc cagaacagcc ctgcatgttg ctgctgcaga
1861 aggtcatgtt gaagttgtga agtttttgct ggaagcttgc aaagttaacc ccttccccaa
1921 ggacaggtgg aataacaccc ccatggatga agcactgcac tttggacacc atgatgtttt
1981 taaaatcctc caggaatacc aagttcagta tacacctcaa ggggattctg acgatggaaa
2041 agaaaaccag actgtccaca agaatcttga cgggttgtta taatggtctg aatcgtgaga
2101 cttacattac ttacctagcc attgtggaac atggctatgg agaacatgtg tgtttctatc
2161 tggtagtaat gtgtatttac aacatctgtc cctgcagtgt taccggagcc tccttcattg
2221 tgcgcacacg acaaatctga gttctttggg aaaaaaaata gaaatgaagt agcctccctt
2281 cacaatgtga gcaatagtta cctcgtgcgt tgtacaattt gatgtaaaag agtagttacc
2341 aatgctagct gaactgtgtg gtttccacgg tttgtgtttt gtacattttc aagccctggt
2401 gatgatatat gctcatatgc attaggattg agctttgttg tacagtctgt ccatagggtc
2461 gatgctgtca ttagacgaaa atagtgctac ctttaagaac tttacacata gatttagttt
2521 gagtgtttga gagaccattg cacttgtgtg gttgagtgtt taaaatgtct atcacctcac
2581 ttcacagtgt gactctttaa atattaaaat aattactact aactgttcat agaatatgct
2641 gattctgatt agattttatc agggaatcta ttaagatatg tttggtgacc aaaatatatg
2701 tgtgactgtg gttctagcac tttgaaactt ttcctttcca tacaaggctt aggccagcct
2761 ctctgtggtg tgtagctgtc ggtccccctc agctgggaaa gagagcactg gcttactatg
2821 cagttttcag gtctcctcag caagccatca agcctcccat ttctgccatc agagatagag
2881 cttggctgtt tatctgagga agatgagcca aaattacatc tcaattagtc atcagtctta
2941 ggagtaaaga cagtgatgac tgcaaggtct tctcagcttc tacaataaag aaaaattcag
3001 aaatgcttcc agttaccaaa gttataatga tatatttagg aaaagctaca aataacattt
3061 acttttaatc ctgatgtcaa atgtctgcat caagatagca ctcctttgtg gcattccagg
3121 tatcttctct tcctctactg cttaactgtt ggtgggctag ctatattgtt caataagaaa
3181 aggacacttt attttaaatt tagacccacc ccttctttgt tttggaaaag gcaatttatg
3241 attaacttgg ataataggta aaaagcaacc ttagataatt gtaatattgt gagaacgtaa
3301 ttgtctagga gtcccacata acgtacccta ttatacctcc aagagatgaa gggtatgtta
3361 gtgaggactt ttgatactaa ccacactttt gcatgcacgc acacacacac acacacacac
3421 acacacacac acacacacac acacacacgg atttcttgga aaatgcttta tgaaattagc
3481 aacattctca actgcacaga tactactggt tcccacagta acacagcatc ttctgtgacc
3541 ctcactatct gcactgattg catccaagct ttatgtctgg tggctcatta ttcactccag
3601 ctgctttgat cctgtttcaa agtcagcttc ggccatcccc tccattccct agtgttcctt
3661 cactaagaca aggcaccact aacgtgtttc taagctccag agtctgtggg gaggatgtga
3721 gtggcagcca gccctgtcac tgtgttcctc cttccacacc cctcaggaca ggtggtgggt
3781 actcggagca tcctccccag gaacctcctc caaggttcag gggtgagcgc gctgcagtca
3841 gcggacatac cactgtgcag cagcagccgg ctttccggaa ttcagcgtgt gacttaaata
3901 ttgccagtga ctttaaggat tccctccgat aatggtcttg tatattatct gagacaaatg
3961 ttttcatctc tcctcagctc agtgctgctt catactgtct ctgtggcttg gttgagtgcg
4021 ctctttgtcc tgggccaggt cttgctctgg ctgttcgctg ctggctgata acagcaagga
4081 ccctgtaagt gtgtgaatga gccgctgact gctgccatca gtaaactcca aagacgtctg
4141 ttctggcttt acaaccctag gtgatggttc tgtctctcag aaccattaca tgatcgtgtg
4201 gcttcaacga tctctgcata ccgctgtccc tgctgtcccg gttctgacgc ccctgtctgc
4261 tgatgtgcta cgcccactac tgcctacact gctttagcgg cagagctgga ctcactgtcg
4321 atccagacgt ctgtcaggga cctgataacc gatgtctgcc ctaggtccct gctggggagt
4381 ccacagctat gaatgtattt acttctaaca tttcccaaaa tgaaatctta aaactataaa
4441 ttgtaagtat tctgaaattg ggaaatactt attttaaatg agatcaggta gtgttgcttt
4501 ttacagcata ataaatatgt gtattg
//