GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS HUMTCRGVJ 594 bp ds-DNA PRI 30-MAY-1991
DEFINITION Human rearranged gene for T-cell receptor gamma chain TRGV9-J1
previously designated V(d)-J1 (subgroup II)
ACCESSION X03436
KEYWORDS N-region; T-cell receptor; T-cell receptor gamma; joining region;
rearranged DNA; variable region.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 594)
AUTHORS Lefranc,M.-P., Forster,A. and Rabbitts,T.H.
TITLE Rearrangement of two distinct T-cell gamma-chain variable-region
genes in human DNA
JOURNAL Nature 319, 420-422 (1986)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Bernard,F., Chuchana,P., Lefranc,G. and Lefranc,M.P.
TITLE An STS in the human T cell receptor gamma locus (located at
7p14-15)
JOURNAL Nucleic Acids Res. 18, 6170-6170 (1990)
STANDARD full staff_review
COMMENT *source: cell line=Karpas 1010; clone=lambda K20; splice site at
pos. 164/165 is according EMBO J. 7:719-726 (1988) and has been
confirmed by cDNA sequences
Data kindly reviewed (06-JUL-1988) by Lefranc M.-P.
From EMBL entry HSTCRGVJ; dated 30-OCT-1990.
FEATURES Location/Qualifiers
mRNA <1..48
/note="T-cell receptor gamma chain TRGV9-J1 exon 1"
CDS <522..521
/note="mat. T-cell gamma chain"
/codon_start=522
misc_feature 3..48
/note="variable region V9"
CDS 3..48
/note="T-cell receptor gamma chain precursor (54 is 1st
base in codon)"
/codon_start=3
CDS 3..48
/note="signal peptide (54 is 1st base in codon)"
/codon_start=3
intron 49..158
/note="intron"
mRNA 159..521
/note="T-cell receptor gamma chain TRGV9-J1 exon 2"
misc_feature 159..469
/note="variable region V9"
CDS 159..>158
/note="signal peptide (159 is 2nd base in codon)"
/codon_start=159
CDS 159..521
/note="T-cell receptor gamma chain precursor (159 is 2nd
base in codon)"
/codon_start=159
misc_feature 470..474
/note="N-region"
misc_feature 475..521
/note="joining region J1"
intron 522..>594
/note="intron"
BASE COUNT 172 a 126 c 121 g 175 t
ORIGIN
1 acatgctgtc actgctccac acatcaacgc tggcagtcct tggggctcgt aagtagtttt
61 gctcccccaa tcactttgga ctgatattct ctattgttat atatttttat aaattccaaa
121 ttcttggttt atttactccc tcccattttt ttccccagtg tgtgtatatg gtgcaggtca
181 cctagagcaa cctcaaattt ccagtactaa aacgctgtca aaaacagccc gcctggaatg
241 tgtggtgtct ggaataaaaa tttctgcaac atctgtatat tggtatcgag agagacctgg
301 tgaagtcata cagttcctgg tgtccatttc atatgacggc actgtcagaa aggaatctgg
361 cattccgtca ggcaaatttg aggtggatag gatacctgaa acgtctacat ccactctcac
421 cattcacaat gtagagaaac aggacatagc tacctactac tgtgccttgc gaggttatta
481 taagaaactc tttggcagtg gaacaacact tgttgtcaca ggtaagtatc ggaagaatac
541 aacatttcca aggtaataga gggaaagcag gaaattatta aactggaata atgt
//