GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS HUMVK01 1000 bp ds-DNA PRI 30-MAY-1991
DEFINITION Human rearranged V(kappa) gene for signal peptide and variable
region
ACCESSION X00965
KEYWORDS immunoglobulin; kappa-immunoglobulin; signal peptide.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 1000)
AUTHORS Klobeck,H.G., Combriato,G. and Zachau,H.G.
TITLE Immunoglobulin genes of the x light chain type from two human
lymphoid cell lines are closely related
JOURNAL Nucleic Acids Res. 12, 6995-7006 (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1000)
AUTHORS Zachau,H.G.
JOURNAL Unpublished (1987)
STANDARD full automatic
COMMENT *source: library=phage lambda EMBL3; clone=lambda Walker; *source:
cell line=Walker (burkitt lymphoma derived); **map: chromosome 2;
From EMBL entry HSVK01; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 294..303
/note="regulatory element dc"
promoter 336..342
/note="TATA-box"
mRNA 389..443
/note="exon 1"
CDS 389..443
/note="signal peptide part 1 (443 is 1st base in codon)"
/codon_start=389
intron 444..567
/note="intron I"
mRNA 568..902
/note="exon 2"
CDS 568..578
/note="signal peptide part 2 (568 is 2nd base in codon)"
/codon_start=568
CDS 579..902
/note="variable region"
/codon_start=579
CDS 864..902
/note="joining segment Jk5"
/codon_start=864
BASE COUNT 257 a 249 c 217 g 277 t
ORIGIN
1 cccaccctct aagacttcat tagacattcc ctatgaatgg ttatactctc ctgtatactc
61 ccaatgcaac tctaaaatat attattccat atagtcctta ggtttgtatt aaagtttgac
121 tttttccttc aaaatatctc ttgtcacaac agcggctcta gagagaaata cattccctcc
181 aggcaaatct acggcgctgg tctgacctgg gaccctggga cattgcccct gtgctgagtt
241 actaagatga gccagcctgc agctgtgctc agcctgcccc atgccctgct gattgatttg
301 catgtccaga gcacagcccc ctgccctgaa gactttttta tgggctggtc cgaccctgtg
361 caggagtcag tctcagtcag gacacagcat ggacatgagg gtccccgctc agctcctggg
421 gctcctgcta ctctggctcc gaggtaagga tggagaacac taggaattta ctcagccagt
481 gtgctcagta ctgactggaa cttcagggaa gttctctgat aacatgatta atagtaagaa
541 tatttgtttt tatgtttcca atctcaggtg ccagatgtga catccagatg acccagtctc
601 catcctccct gtctgcatct gtaggagaca gagtcaccat cacttgccgg gcaagtcaga
661 gcattagcaa ttatttaaat tggtatcaac agaaaccagg gaaagcccct aagctcctga
721 tctatgctgc atccagtttg caaagtgggg tcacatcaag gttcagtggc agtggatctg
781 ggacagactt cactctcacc atcagcagtc tgcaacctga agattctgca acttactact
841 gtcaacagag ttacagtacc ctgatcacct tcggccaagg gacacgactg gagattaaac
901 gtaactaatt tttcactatt gtcttctgaa atttgggtct gatggccagt attgacttta
961 cagtcttaaa taggagtttg gtaaacattg gtaaatgagg
//GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS HUMVK02 1000 bp ds-DNA PRI 30-MAY-1991
DEFINITION Human rearranged V(kappa) gene (signal peptide and variable region)
ACCESSION X00966
KEYWORDS immunoglobulin; kappa-immunoglobulin.
SOURCE Homo sapiens DNA.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 1000)
AUTHORS Klobeck,H.G., Combriato,G. and Zachau,H.G.
TITLE Immunoglobulin genes of the x light chain type from two human
lymphoid cell lines are closely related
JOURNAL Nucleic Acids Res. 12, 6995-7006 (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1000)
AUTHORS Klobeck,H.G.
JOURNAL Unpublished (1987)
STANDARD full automatic
COMMENT *source: library=phage lambda charon 30; clone=lambda Daudi-1;
*source: cell line=Daudi; **map: chromosome 2;
From EMBL entry HSVK02; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 294..303
/note="regulatory element dc"
promoter 336..342
/note="TATA-box"
mRNA 389..443
/note="exon 1"
CDS 389..443
/note="signal peptide part 1 (443 is 1st base in codon)"
/codon_start=389
intron 444..567
/note="intron I"
mRNA 568..902
/note="exon 2"
CDS 568..578
/note="signal peptide part 2 (568 is 2nd base in codon)
(578 is 1st base in codon)"
/codon_start=568
CDS 579..902
/note="variable region"
/codon_start=579
CDS 864..902
/note="joining segment Jk4"
/codon_start=864
BASE COUNT 252 a 250 c 209 g 289 t
ORIGIN
1 cccaccctct aagacttcat tagacattcc ctatgaatgg ttatactctc ctgtatactc
61 ccaatgcaac tctaaaatat attattccat atagtcctta ggtttgtatt aaagtttgac
121 tttttccttc aaaatatctc ttgtcacaac agcggctcta gagagaaata cattccctcc
181 aggcaaatct acggcgctgg tctgacctgg gaccctggga cattgcccct gtgctgagtt
241 actaagatga gccagcctgc agctgtgctc agcctgcccc atgccctgct gattgatttg
301 catgtccaga gcacagcccc ctgccctgaa gactttttta tgggctggtc cgaccctgtg
361 caggagtcag tctcagtcag gacacagcat ggacatgagg gtccccgctc agctcctggg
421 gctcctgcta ctctggctcc gacgtaagga gggagaacac taggaattta ctcagccagt
481 gtgctcagca ctgactggaa cctcagggaa tttctctgat aacatgatta atagtaagaa
541 tatttgtttt tatatttcca atctcaggtg tcagatgtga catccagatg acccagtctc
601 catcctccct gtctgcatct gtaggagaca gagttaccat cacttgccgg gcaggtcata
661 acattaccaa ctttttaagt tggtatcagc aaaaaccagg caaagcccct acattattga
721 tttatgctgt gtccaatttg caagttgggg tcccatcaag attcagtggc agtggatctg
781 gggcagaatt cactctcacc atcagcagtc tacaacctga agattttgca acctactact
841 gtcaacagaa ttacaatttc tcgttcactt tcggcggagg gaccaaggtg gacaacaaac
901 gtgagtgcaa ctttgctgat ttttttctta tacattttta gaattggagc gcttttgtgt
961 ttgagatatt acttaggtca cttccacaga gcatcaagtt
//