GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS HUMGCSFA 246 bp ss-mRNA PRI 30-MAY-1991
DEFINITION Human granulocyte-colony stimulating factor mRNA, partial cds.
ACCESSION M20922
KEYWORDS granulocyte-colony stimulating factor.
SOURCE Human LD-1 cell, cDNA to mRNA, clone pJD1.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 246)
AUTHORS Devlin,P.E., Drummond,R.J., Toy,P., Mark,D.F., Watt,K.W. and
Devlin,J.J.
TITLE Alteration of amino-terminal codons of human granulocyte-colony-
stimulating factor increases expression levels and allows efficient
processing by methionine aminopeptidase in Escherichia coli
JOURNAL Gene 65, 13-22 (1988)
STANDARD simple staff_review
FEATURES Location/Qualifiers
misc_feature complement(1..217)
/note="putative VECTOR sequence Bacteriphage lambda
(J02459)"
misc_feature 211..216
/note="ribosome binding site (put.)"
CDS 223..>246
/note="granulocyte-colony stimulating factor precursor"
/codon_start=223
mat_peptide 226..>246
/note="granulocyte-colony stimulating factor"
/codon_start=226
BASE COUNT 70 a 64 c 66 g 46 t
ORIGIN 217 bp upstream of HindIII site.
1 atcagcagga cgcactgacc accatgaagg tgacgctctt aaaaattaag ccctgaagaa
61 gggcagcatt caaagcagaa ggctttgggg tgtgtgatac gaaacgaagc attggccgta
121 agtgcgattc cggattagct gccaatgtgc caatcgcggg gggttttcgt tcaggactac
181 aactgccaca caccaccaaa gctaactgac aggagaaagc ttatgacccc cctgggccct
241 gccagc
//