GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS BACBANI 3106 bp ds-DNA BCT 30-MAY-1991
DEFINITION Bacillus aneurinolyticus, BanI restriction-modification genes.
ACCESSION D00704
KEYWORDS BanI restriction-modification genes; methyltransferase;
restriction endonuclease.
SOURCE Bacillus aneurinolyticus, IAM1077, chromosomal DNA, clone pBanIRM8.
ORGANISM Bacillus aneurinolyticus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 3106)
AUTHORS Maekawa,Y., Yasukawa,H. and Kawakami,B.
TITLE Cloning and nucleotide sequences of the BanI
restriction-modification genes in Bacillus aneurinolyticus
JOURNAL J. Biochem. 107, 645-649 (1990)
STANDARD full staff_entry
COMMENT BanI methlase contains sequences common to cytosine-specific
methylasea.
FEATURES Location/Qualifiers
misc_binding 491..495
/note="ribosome binding sequence of BanI restriction gene"
CDS 502..1566
/note="BanI restriction endonuclease"
misc_binding 1576..1579
/note="ribosome binding sequence of BanI modification
gene"
CDS 1585..2871
/note="BanI modification methylase"
misc_signal 2881..2903
/note="transcritional termination signal"
BASE COUNT 1029 a 523 c 640 g 914 t
ORIGIN 1625 bp upstream of EcoRI site.
1 atcaaatgca gatgttatcg tcgtggaccc gccgcgcaaa ggctgtgatg agcggttgtt
61 ggacaccatt gtagccatga agcccgagcg cgtagtatat gtatcatgta atccggctac
121 cctggcgcgc gacctgcgta ttctggaaga tgggggttac aggacgatag aggtgcagcc
181 gtggacatgt ttccccatac gaatcatgtg gagtgctgtt cactgttggt caggaatgaa
241 aactaatatg gagatatatt ggcgaaaatc aaaaaggagc gggtaatgct actgatcggt
301 agccccgctt ttatttttgt gcgcacaact attcaattaa agtagggtac ggatttaaca
361 acacttacac tggaaatcag aaactatttt ttgtgaatcg acaccccgtg tcctctataa
421 attattgcta gtgtgtcgaa acaagagtaa tgcgacaaaa aatgatataa tatagaaaaa
481 ctttggaggt gaggatataa gatggcacag cttaaataca ataaagatat tgatgaattg
541 gaaagaaacg ctgcaaagtg gtggccggat tttctagcta aaaaggagtc gtcaacgagc
601 attattccta aactggtcga atcgcaagat gcttttatat ctttattaaa tctcagtaag
661 aacaaccctt tcgacatttt ccaactaata gacgcatcta agttcccacc caatctgttt
721 ctaaagcatc ttgttgtatt aaccgacttt ggtggagagc cactaaatcg cttgaatcaa
781 aactttgatt cgctttttcc catgattccc tatggaatcc attacataac aaaagtgtta
841 gggaaatttg aatttttttg gaatgagaaa aaatatgaat atgtttttca agaacttccg
901 gttacatcat taactaattc aaaactcaag attgacggag caagtatttc taagacagtc
961 ccactttctg atttatataa agatgtaatt gtattactca tgttcggtgc taatgctgtt
1021 aattcggaag tatccgaagt tttgatgaaa tgtgaagttg gaaatctcat tggtaagact
1081 gacgagttga agaagtttat taaggagcgt tatatttttg taagcagaat aacaggaggg
1141 gctgaagcaa atacgttagg tcaagtcgct caaacccatg taatagattt tctaagaact
1201 cgattcgggt ctaagggtca cgatataaaa agtaatggac acattgaagg tgtgacccat
1261 aacgatggac aaacactaac cacttttgat gtagtcataa aaaaaggcag caaaagcgtt
1321 gcgattgaga ttagcttcca ggtaactaca aatagtacca tcgagcgtaa agctggacaa
1381 gctaaagcac gatacgatat ggttagtgat actggcaatt atatagctta tattatcgat
1441 ggagcgggga acttccaacg taaaaatgca atcacaacca tttgtaataa tagccattgc
1501 acagtagctt ataccgaaga agaactaaat gtcttgctaa aattcatctt agaaaaactt
1561 gaatagttat ttggggaggc aactatgaag attaaatttg ttgacttgtt tgctggaatt
1621 ggaggaattc gaataggatt tgaaagagcg gccaaacggt ttgaattgga gactgagtgt
1681 gtactttcta gtgagatcga caaaaaggct tgcgaaacat atgcccttaa ttttaaagag
1741 gaacctcaag gtgatataca cgaaattacc agctttccag aattcgattt tcttcttgct
1801 ggatttcctt gtcaaccctt ttcatatgca ggtaaacaac aaggatttgg ggatacaaga
1861 ggaacattat tttttgaggt ggagagggtt cttagggata accgcccaaa ggctttctta
1921 ttagaaaatg ttcggggact ggtaacacac gacaaaggaa gaactttaaa gacgatcatt
1981 tcaaaattag aggaattggg ttacggggtt agctacctat tacttaacag tagtacgttt
2041 ggtgtccccc aaaaccgagt aaggatttat attttaggca tactcggaag taaaccaaag
2101 ttaactctaa cctctaatgt tggagcagcc gattctcaca agtataagaa tgaacaaata
2161 tctttatttg atgaaagcta tgctactgtt aaagacattt tagaagattc tccaagtgaa
2221 aaatatcgct gttctgacga atttatcggt caactttcaa aggttgttgg taataacttt
2281 gaattgctac atggctatcg cttaattgat tatcgtggcg gtaattcgat tcattcttgg
2341 gaacttggca tcaagggtga ttgtacaaaa gaggaaattg aatttctaaa tcagttgatt
2401 gcaaatagaa gaaagaaaat ttatggtacg caccaagacg gaaaagcctt aactttagag
2461 caaattcgga cgttttataa ccacgatcag cttgaggtaa taataaaatc cctactccaa
2521 aaaggatatt taagagaaga agaaaataaa tttaatcccg tttgtgggaa tatgtctttt
2581 gaggtcttta agttccttga cccagacagt atttcaatca cactcacatc tagcgatgct
2641 cataagttgg gagtagttca gaataatgtt cctagaagga taacacctcg ggaatgtgct
2701 agactgcaag gtttcccaga tgatttcata ctacattcca atgacaattt tgcatacaag
2761 cagttgggga attcagtcac tgtcaaagta gtggaaaaag tgatcgagga tttatttcaa
2821 aacaatgtaa atgaattgtt tggacagatg aaacttgcaa atgttgtatg aatataaatt
2881 aaccccacaa ttattgtggg gttgtttatg tcactatttg tttcagtttc aaccaatcaa
2941 tgaatagtca atatctaaac taggtaaaca tgggttattt ttcaaaatat caactaattt
3001 catacgattg tccaacgtac tttccttata aaatcctctt atcccgccca taccataaac
3061 ctgcattagt tcacaaaaac caataagagt ttcaatttta ttgatc
//