GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS COBS 4200 bp ss-RNA VRL 30-MAY-1991
DEFINITION Bovine enteric coronavirus, spike glycoprotein gene.
ACCESSION D00731
KEYWORDS S protein; spike glycoprotein.
SOURCE Bovine enteric coronavirus (strain F15) grown in HRT18 cells, cDNA
to viral RNA, clones P G7, P G7 8, P G7 8 12, P 27 40, and P 33 23.
ORGANISM Bovine coronavirus
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Coronaviridae.
REFERENCE 1 (bases 1 to 4200)
AUTHORS Boireau,P., Cruciere,C. and Laporte,J.
TITLE Nucleotide sequence of glycoprotein S gene of bovine enteric
coronavirus and comparison with the S proteins of two mouse
hepatitis virus strains
JOURNAL J. Gen. Virol. 71, 487-492 (1990)
STANDARD full staff_review
COMMENT The predicted protein has 19 N-glycosylation sites (specific for
the Asn-X-Ser/Thr sequence).
FEATURES Location/Qualifiers
misc_signal 23..32
/note="conserved intergenic sequence"
CDS 31..4122
/note="spike glycoprotein precursor"
sig_peptide 31..81
/note="spike glycoprotein signal peptide (putative)"
mat_peptide 82..4119
/note="spike glycoprotein mature peptide"
misc_feature 2334..2335
/note="tryptic cleavage site (putative)"
misc_feature 3943..4038
/note="C-terminal membrane anchoring domain"
BASE COUNT 1176 a 684 c 819 g 1521 t
ORIGIN 514 bp upstream of AccI site.
1 gctgcatgat gcttagacca taatctaaac atgtttttga tacttttaat ttccttacca
61 atggctcttg ctgttatagg agatttaaag tgtactacgg tttccattaa tgatgttgac
121 accggtgttc cttctattag cactgatact gtcgatgtta ctaatggttt aggtacttat
181 tatgttttag atcgtgtgta tttaaatact acgctgttgc ttaatggtta ctaccctact
241 tcaggttcta catatcgtaa tatggcactg aagggaactt tactattgag cacactatgg
301 tttaaaccac cttttctttc tgattttatt aatggtattt ttgctaaggt caaaaatacc
361 aaggttatta aacatggtgt aatgtatagt gagtttcctg ctataactat aggtagtact
421 tttgtaaata catcctatag tgtggtagta caaccacata ctaccaattt agataataaa
481 ttacaaggtc tcttagagat ctctgtttgc cagtatacta tgtgcgagta cccaaatacg
541 atttgtcatc ctaatttggg taatcggcgc gtagaactat ggcattggga tacaggtgtt
601 gtttcctgtt tatataagcg taacttcaca tatgatgtga atgctgatta tttgtatttc
661 catttttatc aagaaggtgg tactttttat gcatatttta cagatactgg tgttgttact
721 aagtttctgt ttaatgttta tttaggcacg gtgctttcac attattatgt catgcctttg
781 acttgtaata gtgctatgac tttagaatat tgggttacac ctctcacttc taaacaatat
841 ttactcgctt tcaatcaaga tggtgttatt tttaatgctg ttgattgtaa gagtgatttt
901 atgagtgaga ttaagtgtaa aacactatct atagcaccat ctactggtgt ttatgaatta
961 aacggttaca ctgttcagcc aattgcagat gtttaccgac gtatacctaa tcttcccgat
1021 tgtaatatag aggcttggct taatgataag tctgtgccct ctccattaaa ttgggaacgt
1081 aagacctttt caaattgtaa ttttaatatg agcagcctga tgtcttttat ccaggcagac
1141 tcatttactt gtaataatat tgatgctgct aagatatatg gtatgtgttt ttccagcata
1201 actatagata agtttgctat acccaatggt aggaaggttg acctacaatt gggcaatttg
1261 ggctatttgc agtcttttaa ctatagaatt gatactactg ctacaagttg tcagttgtat
1321 tataatttac ctgctgctaa tgtttctctc agcaggttta atccttctat ttggaatagg
1381 agatttggtt ttacagaaca atctgttttt aagcctcaac ctgtaggtgt ttttactgat
1441 catgatgttg tttatgcaca acattgtttt aaagctccca caaatttctg tccgtgtaaa
1501 ttggatgggt ctttgtgtgt aggtaatggt cctggtatag atgctggtta taaaaatagt
1561 ggtataggca cttgtcctgc aggcactaat tatttaactt gccataatgc tgcccaatgt
1621 aattgtttgt gcactccaga ccccattaca tctaaatcta cagggcctta caagtgcccc
1681 caaactaaat acttagttgg cataggtgag cactgttcgg gtcttgctat taaaagtgat
1741 tattgtggag gtaatccttg tacttgccaa ccacaagcat ttttgggttg gtctgttgac
1801 tcttgtttac aaggggatag gtgtaatatt tttgctaatt ttattttgca tgatgttaat
1861 agtggtacta cttgttctac tgatttacaa aaatcaaaca cagacataat tcttggtgtt
1921 tgtgttaatt atgatcttta tggtattact ggccaaggta tttttgttga ggctaatgcg
1981 acttattata atagttggca gaacctttta tatgattcta atggtaatct ctatggtttt
2041 agagactact taacaaacag aacttttatg attcgtagtt gctatagcgg tcgtgtttca
2101 gcggcctttc atgctaactc ttccgaacca gcactgctat ttcggaatat taaatgcaat
2161 tacgttttta ataatactct ttcacgacag ctgcaaccta ttaactattt tgatagctat
2221 cttggttgtg ttgtcaatgc tgataatagt actgctagtg ctgttcaaac atgtgatctc
2281 acagtaggta gtggttactg tgtggattac tctacaaaaa gacgaagcgt aagagcgatt
2341 accactggtt atcggtttac taattttgag ccatttactg ttaattcagt aaatgatagt
2401 ttagaacctg taggtggttt gtatgaaatt caaatacctt cagaatttac tataggtaat
2461 atggaggagt ttattcaaac aagctctcct aaagttacta ttgattgttc tgcttttgtc
2521 tgtggtgatt gtgcagcatg taaatcacag ttggttgaat atggtagttt ctgtgacaat
2581 attaatgcta tactcacaga agtaaatgaa ctacttgaca ctacacagtt gcaagtagct
2641 aatagtttaa tgaatggtgt cactcttagc actaagctta aagatggcgt taatttcaat
2701 gtagacgaca tcaatttttc ccctgtatta ggttgtttag gaagcgaatg taataaagtt
2761 tccagtagat ctgctataga ggatttactt ttttctaaag taaagttatc tgatgtcggt
2821 ttcgttgagg cttataataa ttgtactggg ggtgccgaaa tcagggacct catttgcgtg
2881 caaagttata atggtatcaa agtgttgcct ccactgctct cagaaaatca gatcagtgga
2941 tacactttgg ctgctacctc tgctagtctg tttcctcctt ggtcagcagc agcaggtgta
3001 ccattttatt taaatgttca gtatcgtatt aatgggattg gtgttaccat ggatgtgcta
3061 agtcaaaatc aaaagcttat tgctaatgca tttaacaatg ctcttgatgc tattcaggaa
3121 gggtttgatg ctaccaattc tgctttagtt aaaattcaag ctgttgttaa tgcaaatgct
3181 gaagctctta ataacttatt gcaacaactc tctaatagat ttggtgctat aagttcttct
3241 ttacaagaaa ttctatccag acttgatgct cttgaagcgc aacgtcagat agacagactt
3301 attaatgggc gtttcaccgc tcttaatgct tatgtttctc aacagcttag tgattctaca
3361 ctagtaaaat ttagtgcagc acaagctatg gagaaggtta atgaatgtgt caaaagccaa
3421 tcatctagga taaatttttg tggtaatggt aatcatatta tatcattagt gcagaatgct
3481 ccatatggtt tgtattttat ccattttagc tatgtcccta ctaagtatgt caccgcgaag
3541 gttagtcccg gtctgtgcat tgctggtgat agaggtatag cccctaagag tggttatttt
3601 gttaatgtaa ataacacttg gatgttcact ggtagtggtt attactaccc tgaacctata
3661 actggaaata atgttgttgt tatgagtacc tgtgctgtta attacactaa agcgccggat
3721 gtaatgctga acatttcaac acccaacctc cctgatttta aggaagagtt ggatcaatgg
3781 tttaaaaacc aaacatcagt ggcaccagat ttgtcacttg attatataaa tgttacattc
3841 ttggacctac aagatgaaat gaataggtta caggaggcaa taaaactttt aaatcagagc
3901 tacatcaatc tcaaggacat tggtacatat gagtattatg taaaatggcc ttggtatgta
3961 tggcttttaa ttggctttgc tggtgtagct atgcttgttt tactattctt catatgctgt
4021 tgtacaggat gtgggactag ttgttttaag aaatgtggtg gttgttgtga tgattatact
4081 ggacaccagg agttagtaat taaaacatca catgacgact aagttcgtct ttgatttatt
4141 ggctcctgac gatatattac atcccttcaa tcatgttaag ttaattatta taagcccatt
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