GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS COBSQUE 4125 bp ss-RNA VRL 30-MAY-1991
DEFINITION Bovine coronavirus, S peplomer gene, complete cds.
ACCESSION D00662
KEYWORDS peplomer gene.
SOURCE Bovine coronavirus (Quebec strain) genomic RNA.
ORGANISM Bovine coronavirus
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Coronaviridae.
REFERENCE 1 (bases 1 to 4125)
AUTHORS Parker,M.D., Yoo,D., Cox,G.J. and Babiuk,L.A.
TITLE Primary structure of the S peplomer gene of bovine coronavirus and
surface expression in insect cells
JOURNAL J. Gen. Virol. 71, 263-270 (1990)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Boireau,P., Cruciere,C. and Laporte,J.
TITLE Nucleotide sequence of the glycoprotein S gene of bovine enteric
coronavirus and comparison with the S proteins of two mouse
hepatitis virus strains
JOURNAL J. Gen. Virol. 71, 487-492 (1990)
STANDARD full staff_review
REFERENCE 3 (sites)
AUTHORS Abraham,S., Kienzle,T.E., Lapps,W. and Brian D.A.,.
TITLE Deduced sequence of the bovine coronavirus spike protein and
identification of the internal proteolytic cleavage site
JOURNAL Virology 176, 296-300 (1990)
STANDARD full staff_review
COMMENT
[3] sites.
[2] sites.
These data kindly submitted in computer readable form by:
Michael D. Parker
Veterinary Infectious Disease Organization
124 Veterinary Road
Saskatoon, Saskatchewan S7N OWO
Canada
Phone: 306-966-7482
Fax: 306-966-7478
FEATURES Location/Qualifiers
misc_feature 7..13
/note="conserved intergenic sequence"
CDS 14..4105
/note="S peplomer polypeptide precursor"
sig_peptide 17..64
/note="S peplomer signal peptide"
misc_feature 2303..2317
/note="possible tryptic cleavage site separating S1 and
S2"
BASE COUNT 1151 a 657 c 811 g 1506 t
ORIGIN 69 bp upstream of DraI site.
1 ccataatcta aacatgtttt tgatactttt aatttcctta ccaatggctt ttgctgttat
61 aggagattta aagtgtacta cggtttccat taatgatgtt gacaccggtg ctccctctat
121 tagcactgat attgtcgatg ttactaatgg tttaggtact tattatgttt tagatcgtgt
181 gtatttaaat actacgttgt tgcttaatgg ttactaccct acttcaggtt ctacatatcg
241 taatatggca ctgaagggaa ctttactatt gagcagacta tggtttaaac caccttttct
301 ttctgatttt attaatggta tttttgctaa ggtcaaaaat accaaggtta ttaaaaaggg
361 tgtaatgtat agtgagtttc ctgctataac tataggtagt acttttgtaa atacatccta
421 tagtgtggta gtacaaccac atactaccaa tttggataat aaattacaag gtctcttaga
481 gatctctgtt tgccagtata ctatgtgcga gtacccacat acgatttgtc atcctaagct
541 gggtaataaa cgcgtagaac tatggcattg ggatacaggt gttgtttcct gtttatataa
601 gcgtaatttc acatatgatg tgaatgctga ttacttgtat ttccattttt atcaagaagg
661 tggtactttt tatgcatatt ttacagacac tggtgttgtt actaagtttc tgtttaatgt
721 ttatttaggc acggtgcttt cacattatta tgtcctgcct ttgacttgtt ctagtgctat
781 gactttagaa tattgggtta cacctctcac ttctaaacaa tatttactag ctttcaatca
841 agatggtgtt atttttaatg ctgttgattg taagagtgat tttatgagtg agattaagtg
901 taaaacacta tctatagcac catctactgg tgtttatgaa ttaaacggtt acactgttca
961 gccaattgca gatgtttacc gacgtatacc taatcttccc gattgtaata tagaggcttg
1021 gcttaatgat aagtcggtgc cctctccatt aaattgggaa cgtaagacct tttcaaattg
1081 taattttaat atgagcagcc tgatgtcttt tattcaggca gactcattta cttgtaataa
1141 tattgatgct gctaagatat atggtatgtg tttttccagc ataactatag ataagtttgc
1201 tatacccaat ggtaggaagg ttgacctaca attgggcaat ttgggctatt tgcagtcttt
1261 taactataga attgatacta ctgctacaag ttgtcagttg tattataatt tacctgctgc
1321 taatgtttct gttagcaggt ttaatccttc tacttggaat aggagatttg gttttacaga
1381 acaatttgtt tttaagcctc aacctgtagg tgtttttact catcatgatg ttgtttatgc
1441 acaacattgt tttaaagctc ccaaaaattt ctgtccgtgt aaattggatg ggtctttgtg
1501 tgtaggtaat ggtcctggta tagatgctgg ttataaaaat agtggtatag gcacttgtcc
1561 tgcaggtact aattatttaa cttgccataa tgctgcccaa tgtgattgtt tgtgcactcc
1621 cgaccccatt acatctaaat ctacagggcc ttacaagtgc ccccaaacta aatacttagt
1681 tggcataggt gagcactgtt cgggtcttgc tattaaaagt gattattgtg gaggtaatcc
1741 ttgtacttgc caaccacaag catttttggg ttggtctgtt gactcttgtt tacaagggga
1801 taggtgtaat atttttgcta attttatttt tcatgatgtt aatagtggta ctacttgttc
1861 tactgattta caaaaatcaa acacagacat aattcttggt gtttgtgtta attatgatct
1921 ttatggtatt acaggccaag gtatttttgt tgaggttaat gcgacttatt ataatagttg
1981 gcagaacctt ttatatgatt ctaatggtaa tctctatggt tttagagact acttaacaaa
2041 cagaactttt atgattcgta gttgctatag cggtcgtgtt tcagcggcct ttcatgctaa
2101 ctcttccgaa ccagcattgc tatttcggaa tattaaatgc aattacgttt ttaataatac
2161 tctttcacga cagctgcaac ctattaacta ttttgatagt tatcttggtt gtgttgtcaa
2221 tgctgataat agtacttcta gtgttgttca aacatgtgat ctcacagtag gtagtggtta
2281 ctgtgtggat tactctacaa aaagacgaag tcgtagagcg attaccactg gttatcggtt
2341 tactaatttt gagccattta ctgttaattc agtaaatgat agtttagaac ctgtaggtgg
2401 tttgtatgaa attcaaatac cttcagagtt tactataggt aatatggagg agtttattca
2461 aacaagctct cctaaagtta ctattgattg ttctgctttt gtctgtggtg attatgcagc
2521 atgtaaatca cagttggttg aatatggtag cttctgtgac aatattaatg ctatactcac
2581 agaagtaaat gaactacttg acactacaca gttgcaagta gctaatagtt taatgaatgg
2641 tgtcactctt agcactaagc ttaaagatgg cgttaatttc aatgtagacg acatcaattt
2701 ttcccctgta ttaggttgtt taggaagcgc ttgtaataaa gtttccagca gatctgctat
2761 agaggattta cttttttcta aagtaaagtt atctgatgtc ggtttcgttg aggcttataa
2821 taattgtact ggaggtgccg aaattaggga cctcatttgt gtgcaaagtt ataatggtat
2881 caaagtgttg cctccactgc tctcagtaaa tcagatcagt ggatacactt tggctgccac
2941 ctctgctagt ctgtttcctc ctttgtcagc agcagtaggt gtaccatttt atttaaatgt
3001 tcagtatcgt attaatggga ttggtgttac catggatgtg ttaagtcaaa atcaaaagct
3061 tattgctaat gcatttaaca atgctcttga tgctattcag gaagggtttg atgctaccaa
3121 ttctgcttta gttaaaattc aagctgttgt taatgcaaat gctgaagctc ttaataactt
3181 attgcaacaa ctctctaata gatttggtgc tataagttct tctttacaag aaattctatc
3241 tagactggat gctcttgaag cgcaagctca gatagacaga cttattaatg ggcgtcttac
3301 cgctcttaat gtttatgttt ctcaacagct tagtgattct acactagtaa aatttagtgc
3361 agcacaagct atggagaagg ttaatgaatg tgtcaaaagc caatcatcta ggataaattt
3421 ttgtggtaat ggtaatcata ttatatcatt agtgcagaat gctccatatg gtttgtattt
3481 tatccacttt agctatgtcc ctactaagta tgtcactgcg aaggttagtc ccggtctgtg
3541 cattgctggt gatagaggta tagcccctaa gagtggttat tttgttaatg taaataatac
3601 ttggatgttc actggtagtg gttattacta ccctgaaccc ataactggaa ataatgttgt
3661 tgttatgagt acctgtgctg ttaactatac taaagcgccg gatgtaatgc tgaacatttc
3721 aacacccaac ctccatgatt ttaaggaaga gttggatcaa tggtttaaaa accaaacatc
3781 agtggcacca gatttgtcac ttgattatat aaatgttaca ttcttggacc tacaagatga
3841 aatgaatagg ttacaggagg caataaaagt tttaaatcag agctacatca atctcaagga
3901 cattggtaca tatgagtatt atgtaaaatg gccttggtat gtatggcttt taattggctt
3961 tgctggtgta gctatgcttg ttttactatt cttcatatgc tgttgtacag gatgtgggac
4021 tagttgtttt aagatatgtg gtggttgttg tgatgattat actggacacc aggagttagt
4081 aattaaaaca tcacatgacg actaagttcg tctttgattt attgg
//