GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS ECOISPA 1452 bp ds-DNA BCT 30-MAY-1991
DEFINITION E.coli ispA gene for farnesyl diphosphate synthase (EC 2.5.1.1).
ACCESSION D00694
KEYWORDS farnesyl diphosphate synthase; isoprenoid synthesis; ispA gene.
SOURCE E.coli (strain K12) genomic DNA, clone lambda2H5.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1452)
AUTHORS Fujisaki,S., Hara,H., Nishimura,Y., Horiuchi,K. and Nishino,T.
TITLE Cloning and nucleotide sequence of the ispA gene responsible for
farnesyl diphosphate synthase activity in Escherichia coli
JOURNAL J. Biochem. 108, 995-1000 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Shingo Fujisaki
Faculty of General Education
Gifu University
Yanagido, Gifu-shi
Gifu 501-11
Japan
Phone: 0582-30-1111 x6223
Fax: 0582-30-1105
Two ORFs are transcribed counterclockwise on the chromosome.
FEATURES Location/Qualifiers
misc_signal 178..183
/note="sigma 70 promoter -35 region"
misc_signal 200..205
/note="sigma 70 promoter -10 region"
misc_binding 226..228
/note="pot. SD sequence for ORF1"
CDS 242..484
/note="ORF1"
misc_signal 437..445
/note="sigma 32 promoter -35 region"
misc_signal 459..468
/note="sigma 32 promoter -10 region"
misc_binding 472..474
/note="pot. SD sequence for ORF2"
CDS 484..1383
/note="ORF2 for farnesyl diphosphate synthase"
BASE COUNT 360 a 379 c 388 g 325 t
ORIGIN 10 min on K12 map.
1 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
61 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
121 ggaagtatac cactaacatc gctttgctgt gcacatcacc ttaccattgc gcgttatttg
181 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
241 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
301 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
361 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
421 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
481 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
541 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
601 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttc
661 ggcgttagca caaacacgct ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac
721 tcattaattc atgatgattt accggcaatg gatgatgacg atctgcgtcg cggtttgcca
781 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
841 ctggcgttct cgattttaag cgatgccgat atgccggaag tgtcggaccg cgacagaatt
901 tcgatgattt ctgaactggc gagcgccagt ggtattgccg gaatgtgcgg tggtcaggca
961 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
1021 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
1081 aaaggacgtc gtgctctgcc ggtactcgac aagtatgcag agagcatcgg ccttgccttc
1141 caggttcagg atgacatcct ggatgtggtg ggagatactg caacgttggg aaaacgccag
1201 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
1261 cggaagaaag cccgggatct gatcgacgat gcccgtcagt cgctgaaaca actggctgaa
1321 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
1381 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
1441 gcactggtcg ac
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