GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS STMSMPI 1142 bp ds-DNA BCT 30-MAY-1991
DEFINITION S.nigrescens smpi gene for metalloprotease inhibitor.
ACCESSION D00671
KEYWORDS metalloprotease inhibitor.
SOURCE S.nigrescens (strain TK-23) genomic DNA.
ORGANISM Streptomyces nigrescens
Prokaryota; Bacteria; Firmicutes; Streptomycetaceae.
REFERENCE 1 (bases 1 to 1142)
AUTHORS Tanaka,K., Aoki,H., Oda,K., Murao,S., Saito,H. and Takahashi,H.
TITLE Nucleotide sequence of the gene for a metalloprotease inhibitor
(SMPI) of Streptomyces nigrescens
JOURNAL Nucleic Acids Res. 18, 6433-6433 (1990)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by:
Hideo Takahashi
Institute of Applied Microbiology
University of Tokyo
1-1-1 Yayoi, Bunkyo-ku
Tokyo 113
Japan
Phone: 03-812-2111 x7825/7826
Fax: 03-813-0539
FEATURES Location/Qualifiers
repeat_region 384..398
/note="direct repeat"
misc_binding 423..426
/note="ribosome binding site"
CDS 433..828
/note="precursor of metalloprotease inhibitor"
sig_peptide 433..530
/note="signal peptide of metalloprotease inhibitor"
mat_peptide 531..825
/note="mature peptide of metalloprotease inhibitor"
repeat_region 1009..1050
/note="inverted repeat"
repeat_region 1083..1108
/note="inverted repeat"
BASE COUNT 157 a 349 c 438 g 198 t
ORIGIN
1 gatcttgtgc tgccgcttct gcacaggggt cgtgcggaat ccctggatgt ttcccgtgtg
61 cgggggaatt gttggaaatt gtttttccgt ggggggtgga gagggaggcg gagaaggggg
121 tagaggcctt ggggtgtaag gggcttcttg tcggcgggtg ggggagggga ttcggtcatc
181 gtcggggtgc gggtggcggt gaggggagga atgtggtgcc tgcgggggag tgggttcgcc
241 acaaagaacg gggtgcgggg cgagcgcaac cggacaaaag tgtggtgtcc gctaatggga
301 tgtcgtgagg cgcaatcggg tccatggggt cggcgccgtc cggaactcgt gggcacagtg
361 agtgcggttc gggatttagg gaattccgta gttccgtacc cacgtcatcc accgaagaat
421 tcggagtaga caatggtccg caagcgtgcg ttaggcctcg ccggctcggc cctcaccctc
481 gtgctcggtg ccgtcggctt caccgctccg gcgcaggccg ccccgagctg tccggcgggg
541 agcctgtgta cctacagcgg gaccgggctg tcgggcgccc gcacggtgat cccggcgtcg
601 gacatggaga aggcggggac cgacggcgtg aagctgcccg cgtcggcccg gtcgttcgcc
661 aacgggacac acttcaccct gcggtacggc cccgcgcgga aggtgacctg cgtccgcttc
721 ccgtgctacc agtacgccac ggtcgggaag gtcgcccccg gcgcccagct ccggtccctc
781 ccgtcccccg gcgccaccgt cacggtgggg caggacctcg gcgactaggg gctgtcttcc
841 aaagtccctg cgagcgccga cccggggcgt cctcggtcag ccgcagctcg tagtggaggg
901 tccgttcctt cttgaggcgg tgggccagcg cgaccagcag gccgtggatc agcgggtact
961 tgcgggtcag cgagcgctcc gcgcggtact cgcgggtctg cgggtccagc agccgcaccc
1021 gcgcccggac ggcgggcccg gtgcggccgc gccaggtcgg acgcgcagat ctccaccctc
1081 cgggacagtt catccgcttg acctgtccag cgctgccgta cgtccggaga tagcgtgtcg
1141 cc
//