GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS IRPAP 1164 bp ss-mRNA PLN 30-MAY-1991
DEFINITION Irpex lacteus aspartic proteinase.
ACCESSION D00589
KEYWORDS aspartic proteinase.
SOURCE Irpex lacteus (KY2981), cDNA to mRNA, clone pILAP 4108.
ORGANISM Irpex lacteus
Eukaryota; Plantae; Thallobionta; Basidiomycotina;
Hymenomycetidae.
REFERENCE 1 (bases 1 to 1164)
AUTHORS Kobayashi,H., Sekibata,S., Shibuya,H., Yoshida,S., Kusakabe,I. and
Murakami,K.
TITLE Cloning and Sequence Analysis of cDNA for Irpex lacteus Aspartic
Proteinase
JOURNAL Agric. Biol. Chem. 53, 1927-1933 (1989)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Hideyuki Kobayashi
Institute of Applied Biochemistry
University of Tukuba
1-1-1 Tennohdai, Tukuba
Ibaraki 305
Japan
Phone: 0298-53-6646
Fax: 0298-53-4605
N-terminal amino acid sequence of Irpex lacteus aspartic proteinase
was determined.
FEATURES Location/Qualifiers
CDS <1..1025
/note="aspartic proteinase precursor"
mat_peptide 3..1022
/note="aspartic proteinase mature peptide"
misc_feature 96..98
/note="active site of aspartic proteinase"
misc_feature 576..578
/note="glycosylated site"
misc_feature 636..638
/note="active site of aspartic proteinase"
misc_feature 714..716
/note="glycosylated site"
BASE COUNT 230 a 388 c 263 g 283 t
ORIGIN 50 bp upstream of HincII.
1 gtgccgctgg cagcgtcccc gccaccaacc agctcgtaga ctacgttgtt aacgttggcg
61 tcggctcccc cgccaccact tactctctct tggtcgatac cggtagctcg aacacctggc
121 tcggtgctga caagtcttat gtcaagactt ccacctcgtc tgcgaccagc gacaaagtgt
181 ccgtcaccta cggatccggc tcgttctctg gcactgaata cactgacacc gtcactctcg
241 gaagcttgac gatccccaag caatccatcg gtgttgcctc ccgtgatagc ggattcgatg
301 gtgttgatgg tatcctcggt gtcggccctg tcgaccttac tgtcggtacc ctcagccccc
361 acacctctac ctccatcccc actgtcaccg acaacctctt cagccagggc accatcccca
421 ccaacttgct cgcggtctcc ttcgagccca ccacctccga gtcgagcacc aacggcgagt
481 tgactttcgg tgctactgac tccagcaagt acactggttc cattacttac actcccatca
541 cgagcacctc ccccgcgtct gcgtactggg gtattaacca gacgattcgc tacggctctt
601 ccacctccat cctctcctcc accgccggta tcgtcgacac tggcaccacc ctcacgctca
661 ttgcttctga tgcgttcgcc aagtacaaga aggctactgg tgctgttgct gacaacaaca
721 ctggtctctt gcgcttgacc acggctcagt acgccaacct ccagagcttg ttctttacca
781 ttggtggcca gactttcgag ctgaccgcca acgctcagat ctggccccgt aacctgaaca
841 cggctattgg tggcagcgcc agcagtgttt acctcatcgt tggtgacctc ggctccgaca
901 gcggcgaggg tctcgacttc atcaacggtt tgaccttcct cgagcgtttc tactctgtct
961 acgacaccac gaacaagagg cttggtttgg ccaccacctc cttcaccacc gccacctcca
1021 actagacgtt cgctcacgaa ccgtggcaag ctggaaaagg acgatgaatg tacaatgggt
1081 cggatcattg cactgtttat ggaatatgta tcgtctgtaa cggaattgga atttggaatg
1141 gattgttgta ttgttttctg ctat
//