GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS ECOHISPUR1 4732 bp ds-DNA BCT 30-MAY-1991
DEFINITION E.coli histidine (hisT, 3' end), dedA, dedB, dedC, dedD, dedE
(complete cds.), and amidophosphoribosyltransferase (purF), segment
1.
ACCESSION M68934 J02800
KEYWORDS amidophosphoribosyltransferase; dedA gene; dedB gene; dedC gene;
dedD gene; dedE gene; hisT gene; histidine; purF gene.
SEGMENT 1 of 2
SOURCE E.coli (strain K12) DNA, clone psi210.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4732)
AUTHORS Nonet,M.L., Marvel,C.C. and Tolan,D.R.
TITLE The hisT-purF region of the Escherichia coli K-12 chromosome:
Identification of additional genes of the hisT and purF operons
JOURNAL J. Biol. Chem. 262, 12209-12217 (1987)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS <1..51
/note="hisT protein (AA at 1) /nomgen='hisT'"
/codon_start=1
CDS 134..793
/note="dedA protein /nomgen='dedA'"
/codon_start=134
CDS 949..1863
/note="dedB protein"
/codon_start=949
misc_feature 1872..1898
/note="pot. rho-independent transcription termination
signal"
CDS 1933..3204
/note="dedC protein"
/codon_start=1933
CDS 3221..3856
/note="dedD protein (gtg start codon)"
/codon_start=3221
misc_feature 3986..4013
/note="pot. rho-independent transcription termination
signal"
CDS 4115..4603
/note="dedE protein"
/codon_start=4115
BASE COUNT 1046 a 1138 c 1385 g 1163 t
ORIGIN 156 bp upstream of ClaI site.
1 cggtatgatc ttccaaaacc gccaatgggc ccgctatttc tggcggacta acgagaataa
61 tgctcggaac atttcaggct gtagccaacg tagttacagc ctgaaagatg acgagtacaa
121 ggcataggca aatatggacc tgatttattt cctcatcgat tttatcctgc acattgacgt
181 gcatctggca gagctggtcg cggagtacgg cgtctgggtt tatgccattt tgttcttaat
241 tttgttctgt gaaaccggtc tggtggtaac gccgttttta ccgggtgatt cattgctgtt
301 tgttgccgga gcgttggcat cactggaaac caacgatctc aacgtccata tgatggtggt
361 gctgatgctg atcgccgcga ttgtgggtga cgcggtcaat tacactatcg ggcggttgtt
421 cggtgaaaag ttgttcagta atcccaattc gaaaattttc cgtcgcagtt atctcgacaa
481 aacccatcag ttttatgaga aacatggcgg caaaacgatt attctcgccc gttttgtgcc
541 gatcgtcaga acgttcgccc cgtttgttgc gggaatgggc cacatgtcgt accgtcattt
601 cgccgcttat aacgtgatcg gcgcactgtt gtgggtactg ctttttacct acgcaggcta
661 tttcttcggt acaatcccga tggttcagga taaccttaag ctgctgatcg tcgggattat
721 tgtggtttct attttgccgg gcgtcatcga aataatccgt cacaaacgcg ctgcggcacg
781 cgccgcaaaa taaaaagtaa ctccgcggtt cgaccacttt tttatccaaa gtttcgggct
841 gttatgtttt aatgtgcaac attcatggtc tgttgggggc aaaaatggca ttatgcgtcc
901 ccaaagataa aactggcatc gaaccaggtt cagacagaaa ggtccctaat gagctggatt
961 gaacgaatta aaagcaacat tactcccacc cgcaaggcga gcattcctga aggggtgtgg
1021 actaagtgtg atagctgcgg tcaggtttta taccgcgctg agctggaacg taatcttgag
1081 gtctgtccga agtgtgacca tcacatgcgt atgacagcgc gtaatcgcct gcatagcctg
1141 ttagatgaag gaagccttgt ggagctgggt agcagcgttg agccgaaaga tgtgctgaag
1201 tttcgtgact ccaagaagta taaagaccgt ctggcatctg cgcagaaaga aaccggcgaa
1261 aaagatgcgc tggtggtgat gaaaggcact ctgtatggaa tgccggttgt cgctgcggca
1321 ttcgagttcg cctttatggg cggttcaatg gggtctgttg tgggtgcacg tttcgtgcgt
1381 gccgttgagc aggcgctgga agataactgc ccgctgatct gcttctccgc ctctggtggc
1441 gcacgtatgc aggaagcact gatgtcgctg atgcagatgg cgaaaacctc tgcggcactg
1501 gcaaaaatgc aggagcgcgg cttgccgtac atctccgtgc tgaccgaccc gacgatgggc
1561 ggtgtttctg caagtttcgc catgctgggc gatctcaaca tcgctgaacc gaaagcgtta
1621 atcgctttgc cggtccgcgt gttatcgaac agaaccgttc gcgaaaaact gccgcctgga
1681 ttccagcgca gtgaattcct gatcgagaaa ggcgcgatcg acatgatcgt ccgtcgtccg
1741 gaaatgcgcc tgaaactggc gagcattctg gcgaagttga tgaatctgcc agcgccgaat
1801 cctgaagcgc cgcgtgaagg cgtagtggta cccccggtac cggatcagga acctgaggcc
1861 tgataactga taagggcagg gccactggct ctgccctttt gctattctca ccgtaacgaa
1921 tcagcggata ccatgattat caaacgcact cctcaagccg cgtcgcctct ggcttcgtgg
1981 ctttcttatc tggaaaacct gcacagtaaa actatcgatc tcggccttga gcgcgtgagc
2041 ctggtcgcgg cgcgtcttgg cgtcctgaaa ccagcgccat ttgtgtttac cgttgcgggt
2101 acgaatggca aaggcaccac ctgccgtacg ctggagtcga ttctgatggc ggcagggtac
2161 aaagtgggcg tctacagttc gcctcatctg gtgcgttata ccgagcgcgt acgtgtgcag
2221 ggccaggaat tgccggaatc ggcccacacc gcctcttttg cggagattga atcggcacgc
2281 ggtgatattt ccctgaccta tttcgagtac ggtacgctgt cggcgttgtg gctgttcaag
2341 caggcacaac ttgacgtggt gattctggaa gtagggctgg gcggtcgtct ggacgcaacc
2401 aatattgtcg acgccgatgt cgcggtagta accagtattg cgctggatca taccgactgg
2461 ctgggtccag atcgcgaaag tattggtcgc gagaaagcag gcatcttccg cagcgaaaaa
2521 ccggcaattg tcggtgagcc ggaaatgcct tctaccattg ctgatgtggc gcaggaaaaa
2581 ggtgcactgt tacaacgtcg gggcgttgag tggaactatt ccgtcaccga tcatgactgg
2641 gcgtttagcg atgctcacgg cacgctggaa aatctgccgt tgccgcttgt cccgcaaccg
2701 aatgccgcaa cagcgctggc ggcactgcgt gccagcgggc tggaagtcag tgaaaatgcc
2761 attcgcgacg ggattgccag cgcaattttg ccgggacgtt tccagattgt gagcgagtcg
2821 ccacgcgtta tttttgatgt cgcgcataat ccacatgcgg cggaatatct caccgggcgt
2881 atgaaagcgc taccgaaaaa cgggcgcgtg ctggcggcgg ttatcggtat gctacatgat
2941 aaagatattg ccggaactct ggcctggttg aaaagcgtgg ttgatgactg gtattgtgcg
3001 ccactggaag ggccgcgcgg tgccacggca gaacaactgc ttgagcattt gggtaacggc
3061 aaatcatttg atagcgttgc gcaggcatgg gatgccgcaa tggcggagcc taaagcggaa
3121 gacaccgtgc tggtgtgtgg ttctttccac acggtcgcac atgtcatgga agtgattgac
3181 gcgaggagaa gcggtggcaa gtaagtttca gaatcggtta gtgggcacga tcgtgctggt
3241 ggcgctgggg gtgattgtac ttccagggct gctggacggg cagaaaaaac attatcagga
3301 tgagttcgcg gctatcccgc tggtgccgaa agcgggcgat cgtgatgagc ctgatatgat
3361 gccagccgcc acccaggcgt taccgacgca gccgccggaa ggcgcagcgg aagaggtgcg
3421 ggcaggtgat gccgcagcac cgtcgctcga tccggccact attgcagcca ataacaccga
3481 gtttgaaccg gaacctgcac cggtcgcccc accgaagccg aaaccggtgg agccgcctaa
3541 accaaaggtt gaagcaccac ctgcgccgaa gccagaaccg aagccggtcg tggaagaaaa
3601 agctgcaccg acgggtaaag cctatgttgt gcaactgggt gcgctgaaaa atgccgataa
3661 agtgaatgag attgtcggta agctgcgcgg tgccggttat cgggtttata cgtcgccatc
3721 cacgccagtg cagggtaaaa ttacccgtat tctggttggg ccggatgcct cgaaagataa
3781 gctgaaaggt tcgctgggtg agttgaagca actttctggc ttaagtggcg tggtaatggg
3841 ctatacgccg aattaatacg gtcttgcctg atgcgacgct ggcgcgtctt atcaggccta
3901 cgcaggggta gaaccgtagg tcggataagg cgtttacgcc gcatccgaca cgcattgccc
3961 gatgccgcaa aggcataaaa agtcgatggc gttgaatatt ttttcagcgc catttttatt
4021 gatgcgcggg aaggaaatcc ctacgcaaac gttttctttt tctgttagaa tgcgccccga
4081 acaggatgac agggcgtaaa atcgtgggac acatatggtc tggattgatt acgccataat
4141 cgcggtgatt gctttttcct ctctggttag cctgatccgc ggctttgttc gtgaagcgtt
4201 atcgctggtg acatggggtt gtgctttctt tgttgccagt cattactaca cttacctgtc
4261 agtctggttt acgggctttg aagacgaact ggttcgaaat gggattgcca tcgcggtact
4321 gtttatcgct accctgatcg ttggtgctat cgtgaacttc gtgataggcc agttggtgga
4381 gaaaacgggg ttgtcaggca ccgatcgggt gctgggcgtc tgtttcggtg cgttgcgcgg
4441 tgtgttgatt gttgctgcca ttctcttctt tctcgactcc tttaccgggg tgtcgaaaag
4501 cgaagactgg agcaaatcac agctgatccc gcaattcagt tttatcatca gatgtttttt
4561 tgattatctg caaagctcgt caagtttctt gcccagagcg taagtgctct gagatgtggc
4621 ttaacgagga aaaagacgta tgtgcggtat tgtcggtatc gccggtgtta tgccggttaa
4681 ccagtcgatt tatgatgcct taacggtgct tcagcatcgc ggtcaggatg cc
//