GenBank-Updates@genbank.bio.net (05/30/91)
LOCUS ECOHISPUR2 1440 bp ds-DNA BCT 30-MAY-1991
DEFINITION E.coli amidophosphoribosyltransferase (purF) gene (segment 2), dedF
gene(complete cds), and argT gene (5' end).
ACCESSION M68935 J02800
KEYWORDS amidophosphoribosyltransferase; dedA gene; dedB gene; dedC gene;
dedD gene; dedE gene; dedF gene; hisT gene; histidine; purF gene.
SEGMENT 2 of 2
SOURCE E.coli (strain K12) DNA, clone psi210.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1440)
AUTHORS Nonet,M.L., Marvel,C.C. and Tolan,D.R.
TITLE The hisT-purF region of the Escherichia coli K-12 chromosome:
Identification of additional genes of the hisT and purF operons
JOURNAL J. Biol. Chem. 262, 12209-12217 (1987)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS order(M68934:4640..4732,1..72)
/note="purF protein (EC 2.4.2.14) /nomgen='purF'
incomplete coding sequenc-1.35 kb gap"
/codon_start=4640
CDS 167..736
/note="dedF protein"
/codon_start=167
CDS 1002..>1440
/note="argT protein /nomgen='argT'"
/codon_start=1002
misc_feature 1345..1363
/note="pot. rho-independent transcription termination
signal"
BASE COUNT 349 a 370 c 349 g 372 t
ORIGIN 156 bp upstream of ClaI site.
1 cgtaatgatg acgccaaagc agtgcaacgt cagagcgaag tggaaaatct cgaaatgcat
61 aacgaaggat gatgccttcg ctgagggtgc cggtctggca ccctgacttg caactcccgc
121 cgaaatcctg caaagtctgc ctgcaagtct gacagggcaa ctatttatga aacgactcat
181 tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg cgcttattac aggttctgcg
241 cgatgtcaca gatatcgaaa cgcatctggt gatgagccag gcagcgcgcc agaccttatc
301 cctcgaaacg gatttttctc tgcgcgaagt gcaggcatta gccgatgtca gccacgatgc
361 gcgcgatatt gccgccagca tctcttccgg ttctttccag acgctgggga tggtgatttt
421 accctgttca atcaaaaccc tttccggcat tgtccatagc tatactgatg gcttactgac
481 ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg gtgctctgcg tgcgtgaaac
541 accattgcac ttaggccatc tgcgtttaat gactcaggcg ggcagaaatc ggtgcggtga
601 ttatgcctcc gttccggcgt tttatcatcg cccgcaatcc cttgatgatg tgataaatca
661 gacggttaat cgtgttcttg accagtttgc gataaccctt cctgaagatc tctttgcccg
721 ctggcagggc gcataactcc gctgttgccc tgtttcaggg caattttgca accgcgatca
781 aatcctcgac attttgtttc tgccattcaa tcgaaacgct gcgattcaac cgctatacct
841 gctatcttca acttcaggac aataatgcaa cgtcttatta acatatttaa cgttgaatgt
901 tactgttgtc gtcaagatgg cataagacct gcatgaaaga gcctgcaaac acacaacaca
961 atacacaaca taaaaaagcc attttcactt gagggttatg tatgaagaag tcgattctcg
1021 ctctgtcttt gttagtcggt ctctccacag cggcttccag ctatgcgccg ctaccggaga
1081 cggtacgtat cggaaccgat accacctacg caccgttctc atcgaaagat gctaaaggtg
1141 attttgttgg ctttgatatc gatctcggta acgagatgtg caaacggatg caggtgaaat
1201 gtacctgggt tgccagtgac tttgacgcgc tgatcccctc actgaaagca aaaaaatcga
1261 ccgctattat ttcgtcgctt tccgttaccg ataaacgtca gcaggagatt gccttctccg
1321 acaagctgta cgccgcagat tctcgtttga ttgcggccaa aggttcaccg attcagccaa
1381 cgctggattc actgaaaggt aaacatgttg gtgtgctgca gggatcaacc caggaagctt
//