PAU@nfl.afrc.ac.uk (09/13/90)
I need a program to assist in designing oligonucleotides for hybridisation probes and PCR primers. The problem is basically this: given a protein alignment (or section thereof) and a codon usage table, what is the most probable DNA sequence of an as yet unidentified gene encoding a protein of the same class. This has to include the probability of the likeliest base at each position, enabling one to slide a window of given width down the probability profile to identify maxima, which can then be used to synthesise probes. Any offers? Martin Drummond, Nitrogen Fixation Lab, Sussex.